ABSTRACT
Background: Cancer/Testis Antigens [CTAs] are a subgroup of tumor-associated antigens which are expressed normally in germ line cells and trophoblast, and aberrantly in a variety of malignancies. One of the most important CTAs is Developmental Pluripotency Associated-2[DPPA2] with unknown biological function. Considering the importance of DPPA2 in developmental events and cancer, preparing a suitable platform to analyze DPPA2 roles in the cells seems to be necessary
Methods: In this study, the coding sequence of DPPA2 gene was amplified and cloned into the retroviral expression vector to produce recombinant retrovirus. The viral particles were transducted to Esophageal Squamous Cell Carcinoma [ESCC] cell line [KYSE-30 cells] and the stable transducted cells were confirmed for ectopic expression of DPPA2 gene by real-time PCR
Results: According to the critical characteristics of retroviral expression system such as stable and long time expression of interested gene and also being safe due to deletion of retroviral pathogenic genes, this system was used to induce expression of DPPA2 gene and a valuable platform to analyze its biological function was prepared. Transduction results clearly showed efficient overexpression of the gene in target cells in protein level due to high level of GFP expression
Conclusion: Such strategies can be used to produce high levels of desired protein in target cells as a therapeutic target. The produced recombinant cells may present a valuable platform to analyze the effect of DPPA2 ectopic expression in target cells. Moreover, the introduction of its potential capacity into the mouse model to evaluate the tumorigenesis of these cancer cells in vivo leads to an understanding of the biological importance of DPPA2 in tumorigenesis. In addition, our purified protein can be used in a mouse model to produce specific antibody developing a reliable detection of DPPA2 existence in any biological fluid through ELISA system
ABSTRACT
Congenital Fibrosis of the Extra Ocular Muscles1 [CFEOM1] is an autosomal dominant condition, caused by mutation in the KIF21A and TUBB3. It is characterized by congenital non-progressive restrictive ophthalmoplegia and ptosis. Mutational analysis of the known genes in such rare diseases by Sanger sequencing not only prevents wasting the time and expenses but also speeds diagnosis process, genetic counseling, and the possibility of prenatal diagnosis. Here, for the first time, association of pathogenic variant c.2860C>T in KIF21A gene in an Iranian family with positive history of CFEOM1A was reported
ABSTRACT
Background and purpose: Minocycline has got the anti-inflammatory and neuroprotective effects. Considering the interaction between cell death and seizure, and on the other hand, Kindling which increases expression NMDA receptors in brain, the aim of this study is to investigate the effect of minocycline on gene expression of NMDA receptor in hippocampus and piriform brain areas on amygdale kindling acquisition in rats.
Materials and Methods: In this experimental study, three animal groups of 24 Wistar rats received kindling stimulations [twice daily within 6 hours intervals] after being stereotaxic operated and taking one week recovery period. In first Group [n=8] animals did not received daily kindling stimulations. Animals of the second and the third Groups [n=8] respectively had been injected by saline [1ml/kg] and minocycline [25 mg/kg], 60 minutes before receiving kindling stimulations. Two hours after last stimulation animal's brains were removed and the changes of NR2A gene subunit of NMDA receptor in the hippocampus and piriform cortex were measured and compared relative to the control group. Datawere analyzed using ANOVA and Tukey post hoc tests at significant level of P<0/05.
Results: Intraperitoneal administration of minocycline before kindling stimulations prevented an increase in the mRNA of NR2A subunit of NMDA receptor in hippocampus and piriform cortex of amygdala kindling.
Conclusion: The results of this study have shown that minocycline administration before electrical stimulation has anticonvulsant effects which are applied through a decrease in expression of NMDA receptors.
ABSTRACT
Background: Allergic rhinitis is a chronic inflammatory disease of the upper airways, this inflammatory response in the nasal mucosa includes an immediate IgE-mediated response. IL-18, a member of the IL-1 family, is known for influencing the balance of IgE level. In present study this problem is investigated that whether immunoglobulin [Ig] E levels in serum are associated with -607A/C SNPs of IL18 promoter.
Materials and Methods: Genotyping for -607A/C SNPs of IL18 promoter was performed using 133 patients with AR and 62 healthy control volunteers from Chaharmahal va Bakhtiari province then serum levels of total IgE were determined by ELISA method. Statistical analysis were carried out using T test and ANOVA test by SPSS version 19.
Results: level of total IgE in Serum were significantly greater in allergic rhinitis patients than controls [P=0.017]. In addition, the total serum IgE level of the individuals with heterozygous genotype of IL18 [-607AC] were significantly higher regarding other investigated polymorphisms [P=0.045].
Conclusion: According to the results of this study, it seems that the IL18 gene polymorphism -607A/C is associated with IgE levels and susceptibility of allergic rhinitis.