Human leptospirosis in the Caribbean, 1997-2005: characteristics and serotyping of clinical samples from 14 countries / Leptospirosis humana en el Caribe entre 1997 y 2005: características y serotipificación de muestras clínicas de 14 países

OBJECTIVE: To determine the frequency of human leptospirosis in the sera of suspected clinical cases sent by 14 Caribbean countries for diagnosis to a regional laboratory in 1997-2005. METHODS: All serum samples were initially tested using the immunoglobulin M (IgM) enzyme-linked immunosorbent assay (ELISA) for leptospirosis. Demographic data (such as age and sex), month of the year and clinical manifestations that had been observed by the attending physician were related to seropositivity. The microscopic agglutination test (MAT) was used to serotype sera using a panel of 23 international serovars. RESULTS: Of 3 455 samples tested, 452 (13.1 percent) were seropositive for IgM antibodies to leptospirosis by the ELISA, with frequencies significantly (P < 0.05; χ2) different across countries and years. Among seropositive patients, the frequency of detection of leptospirosis (23.1 percent) was significantly higher in the age groups 1-20 years and 31-40 years combined compared with other age groups; and in male patients (72.1 percent) compared with female patients (19.7 percent) (P < 0.05; χ2). Chills, jaundice, vomiting, weakness, diarrhea, and kidney failure/problems were significantly (P < 0.05; χ2) exhibited at a higher frequency in seropositive, rather than seronegative patients. Using the MAT on 100 sera tested, 98 (98 percent) were seropositive, of which the serogroup Icterohaemorrhagiae was most prevalent with the detection of serovars Copenhageni (70 percent), Icterohaemorrhagiae (67 percent), and Mankarso (29 percent). CONCLUSIONS: Since only 13.1 percent of the suspected cases of leptospirosis were seropositive for IgM ELISA antibodies, other clinical conditions may have been responsible for the clinical manifestations observed, or the patient may have had chronic leptospirosis (IgG). In the Caribbean, serovars of the serogroup Icterohaemorrhagiae were responsible for most infections in the cases tested.

OBJETIVO: Determinar la frecuencia de leptospirosis humana en el suero de presuntos casos clínicos enviados por 14 países del Caribe a un laboratorio regional para la confirmación del diagnóstico entre 1997 y 2005. MÉTODOS: Todas las muestras de suero se analizaron inicialmente mediante el ensayo inmunoenzimático de adsorción (ELISA) para detectar inmunoglobulina M (IgM) contra Leptospira. Se relacionó la seropositividad con datos demográficos (como la edad y el sexo), el mes del año y las manifestaciones clínicas observadas por el médico a cargo. Se usó la prueba de aglutinación microscópica para serotipificar los sueros con un grupo de 23 serovariedades internacionales. RESULTADOS: De las 3 455 muestras analizadas por ELISA, 452 (13,1 por ciento) fueron seropositivas para anticuerpos IgM contra Leptospira, con frecuencias significativamente diferentes (P < 0,05; χ2) según el país y el año. En los pacientes seropositivos, la frecuencia de detección de leptospirosis (23,1 por ciento) fue significativamente mayor en los grupos etarios de 1 a 20 años y de 31 a 40 años combinados, en comparación con otros grupos de edad; y mayor en los varones (72,1 por ciento) en comparación con las mujeres (19,7 por ciento) (P < 0,05; χ2). Los escalofríos, la ictericia, los vómitos, la debilidad, la diarrea y la insuficiencia o los trastornos renales fueron significativamente más frecuentes (P < 0,05; χ2) en los pacientes seropositivos que en los seronegativos. De los 100 sueros que se analizaron con la prueba de aglutinación microscópica, 98 (98 por ciento) fueron seropositivos, y entre estos el serogrupo Icterohaemorrhagiae fue el más frecuente, con detección de las serovariedades Copenhageni (70 por ciento), Icterohaemorrhagiae (67 por ciento) y Mankarso (29 por ciento). CONCLUSIONES: Ya que solo 13,1 por ciento de los presuntos casos de leptospirosis fueron seropositivos por ELISA para anticuerpos IgM, las manifestaciones clínicas observadas pueden haberse debido a otras enfermedades, o el paciente puede haber tenido leptospirosis crónica (con anticuerpos IgG). En los casos analizados en el Caribe, las serovariedades del serogrupo Icterohaemorrhagiae causaron la mayoría de las infecciones.

Achado de bactérias selecionadas em crianças de Trinidad com doença amigdaliana crônica / Selected bacterial recovery in Trinidadian children with chronic tonsillar disease

Faringoamigdalite na população pediátrica é largamente tratada com antibióticos. OBJETIVO: Estudar a microflora presente na superfície e no núcleo de amígdalas após adenoamigdalectomia eletiva em crianças. MÉTODO: Amígdalas de 102 crianças de Trinidad foram prospectivamente estudadas por meio de culturas e identificações bacteriológicas feitas a partir de amostras das superfícies e núcleos de suas amígdalas entre 2005-2006. RESULTADOS: A partir de 360 amígdalas, foram isolados Streptococcus spp. (51,3 por cento), Staphylococcus spp. (42,3 por cento) e Gram-Negativos (6,4 por cento). A identificação de estafilococos e estreptococos tanto na superfície quanto no núcleo foi semelhante (p>0,05). Encontramos mais (p<0,001) Streptococcus spp. nas superfícies (82,2 por cento) do que nos núcleos (63,3 por cento); a prevalência de estreptococos alfa-hemolíticos foi maior (p<0,001) do que aquela de estreptococos beta-hemolíticos nas superfícies (74,4 por cento vs. 18,6 por cento) do que nos núcleos (58,9 por cento vs. 13,7 por cento). Não houve concordância entre superfícies e núcleos com relação a estreptococos (p<0,0004) e estreptococos alfa-hemolíticos (p<0,007). Estreptococos beta-hemolíticos foram mais identificados (p<0,05) em crianças dentre 6-16 anos do que naquelas entre 1-5 anos de idade (31 por cento e 23,8 por cento vs 12,5 por cento e 8 por cento). A prevalência de S. pyogenes na superfície e no núcleo foi de (84,6 por cento vs 70 por cento) e (50,0 por cento vs 25,0 por cento) em crianças de maior faixa etária e crianças mais novas, respectivamente. Klebsiella spp. (6,6 por cento, 2,2 por cento), Proteus (4,4 por cento, 4,4 por cento) e Pseudomonas (4,4 por cento, 1,1 por cento) cresceram nas superfícies e núcleos, respectivamente. CONCLUSÃO: As superfícies amigdalianas tinham mais estreptococos e estreptococos hemolíticos do que seus núcleos. Crianças mais velhas tiveram mais estreptococos beta-hemolíticos, e são altamente colonizadoras...

Pharyngotonsillitis in children is widely treated with antibiotics. AIM: To examine tonsil surface and core microflora following elective adenotonsillectomy in children. METHODS: Tonsils of 102 Trinidadian children were prospectively examined for surface and core bacteriological culture and identification between 2005-2006. RESULTS: Tonsils (360) yielded 800 isolates of Streptococcus spp. (51.3 percent), Staphylococcus spp. (42.3 percent) and Gram-negative genera (6.4 percent). Surface and core recovery of staphylococci and streptococci were similar (p>0.05). More (p<0.001) surfaces (82.2 percent) than cores (63.3 percent) grew Streptococcus spp.; α-haemolytic Streptococcus prevalence was higher (p<0.001) than ß-haemolytic Streptococcus on surfaces (74.4 percent vs. 18.6 percent) than cores (58.9 percent vs. 13.7 percent). Surfaces and cores were not concordant for streptococci (p<0.0004) and α-haemolytic Streptococcus (p<0.007). Surface and core ß-haemolytic Streptococcus yield was higher (p<0.05) in 6-16 than 1-5 year olds (31 percent and 23.8 percent vs 12.5 percent and 8 percent). S. pyogenes surface and core prevalence was (84.6 percent vs 70 percent) and (50.0 percent vs 25.0 percent) in older and younger children respectively. Klebsiella spp. (6.6 percent, 2.2 percent), Proteus (4.4 percent, 4.4 percent) and Pseudomonas (4.4 percent, 1.1 percent) grew on surfaces and cores respectively. CONCLUSION: Tonsil surfaces yield higher surface than core carriage for streptococci overall and for α haemolytic streptococci. Older children grow more β-haemolytic streptococci and are high colonizers of S. pyogenes. Studies probing the mechanisms of streptococcal adhesions in Trinidadian children are suggested.

Seroprevalence and risk factors of Toxoplasma gondii infection among pregnant women in Trinidad and Tobago

OBJECTIVE: To determine the seroprevalence of toxoplasmosis and the frequency of risk factors for the disease in women attending antenatal clinics in hospitals and local health centers in Trinidad and Tobago. METHODS: From November 2002-September 2003, 232 pregnant women at the antenatal clinics of two large hospitals were sampled during their first trimesters. From October 2003-February 2005, 218 pregnant women at five health care centers were followed through three trimesters, wherever possible, and blood samples collected. Sera were screened for Toxoplasma gondii IgG and IgM immunoglobulins using an enzyme immunoassay. Data on demographics, as well as practices considered to be risk factors for toxoplasmosis, were obtained. RESULTS: Of the 232 women sampled at the two hospital clinics, 83 (35.8 percent) and 8 (3.4 percent) were seropositive for immunoglobulins of T. gondii IgG and IgM, respectively. Of the 218 women at the health centers, 76 (34.9 percent) had evidence of past infection (IgG), while 26 (11.9 percent) were seropositive for IgM immunoglobulin, suggesting new infections during pregnancy. Only having "3 or more" children was significantly associated with infection by T. gondii. CONCLUSION: In Trinidad and Tobago, the seroprevalence of past infection by T. gondii in pregnant women is relatively low (39.3 percent) for patients in both hospital and health center clinics. Consequently, there is an elevated risk of primary infection during pregnancy and the potential for congenital infection.

OBJETIVO: Determinar la seroprevalencia de toxoplasmosis y la frecuencia de los factores de riesgo de la enfermedad en mujeres que asisten a consultas prenatales en hospitales y centros de salud en Trinidad y Tobago. MÉTODOS: Entre noviembre de 2002 y septiembre de 2003 se tomaron muestras de 232 mujeres que asistieron a consultas prenatales en dos grandes hospitales durante su primer trimestre de embarazo. Entre octubre de 2003 y febrero de 2005 se realizó el seguimiento durante tres trimestres, siempre que fue posible, y se tomaron muestras de sangre de 218 embarazadas atendidas en cinco centros de salud. Se analizaron las muestras de suero para detectar anticuerpos de las clases IgG e IgM contra Toxoplasma gondii mediante ensayos inmunoenzimáticos. Se tomó nota de los datos demográficos y las prácticas consideradas factores de riesgo para la toxoplasmosis. RESULTADOS: De las 232 mujeres estudiadas en los dos hospitales, 83 (35,8 por ciento) y 8 (3,4 por ciento) resultaron seropositivas para anticuerpos IgG e IgM contra T. gondii, respectivamente. De las 218 mujeres estudiadas en los centros de salud, 76 (34,9 por ciento) presentaban evidencias de infección pasada (anticuerpos IgG), mientras 26 (11,9 por ciento) eran positivas a anticuerpos IgM, lo que indicaba una infección reciente durante el embarazo. El único factor asociado significativamente con la infección por T. gondii fue tener tres hijos o más. CONCLUSIONES: En Trinidad y Tobago, la seroprevalencia de infección pasada por T. gondii encontrada en mujeres embarazadas que se atendían en los hospitales y los centros de salud fue relativamente baja (39,3 por ciento). Por consiguiente, existe un elevado riesgo de infección primaria durante el embarazo con la posibilidad de infección congénita.

A cross-sectional study of isolates from sputum samples from bacterial pneumonia patients in Trinidad

We determined the frequency of Streptococcus pneumoniae, Haemophilus influenzae, Staphylococcus aureus, and Gram-negative enteric bacteria (GNEB) in pneumonia patients, determined the antibiograms of these pathogens, and investigated the relationship between pneumonia and selected risk factors. Sputum and demographic data were collected from 124 pneumonia patients. Sputum was cultured for S. aureus, GNEB, H. influenzae and S. pneumoniae. The disc diffusion method was used to determine resistance to eight antimicrobial agents. Among the 124 sputum samples, eight (6.5 percent) were positive for S. aureus, 15 (12.1 percent) for GNEB, two (1.6 percent) for S. pneumoniae and one (0.8 percent) for H. influenzae. Hospitals, gender, ethnicity, co-morbidities and symptoms did not significantly (p > 0.05; chi2) affect the prevalence of these bacteria. GNEB infection was most prevalent (47 percent) in patients over 70 years old. Gentamicin and levofloxacin were the most effective against these bacteria.

Microbial contamination of disinfectants and antiseptics in four major hospitals in Trinidad

OBJECTIVE: To assess the microbial contamination of disinfectants and antiseptics in major hospitals on the Caribbean island of Trinidad. METHODS: For this cross-sectional study, disinfectants and antiseptics were sampled from the pharmacy departments, the pediatric/neonatal wards, and the surgical wards of four hospitals. The samples were cultured for aerobic bacteria on nutrient agar using the surface plating method. The antibiotic sensitivity of bacterial isolates was determined by the disk diffusion method, using 14 antimicrobial agents. We studied a total of 180 disinfectant/antiseptic samples: 60 of chlorhexidine gluconate (Hibitane), 60 of chlorhexidine gluconate and cetrimonium bromide (Savlon), and 60 of methylated spirit. RESULTS: Of the 180 samples studied, 11 of them (6.1 percent) were contaminated by aerobic bacteria. All bacteria isolated were Pseudomonas spp. Of the 11 contaminated samples, 6 of them (54.5 percent) occurred at the pharmacy level while 5 (45.5 percent) were from diluted pre-use or in-use samples in the pediatric/neonatal wards or the surgical wards. Chlorhexidine gluconate and cetrimonium bromide accounted for 9 of the 11 contaminated disinfectants/antiseptics (81.8 percent), and chlorhexidine gluconate accounted for the remaining 2 (18.2 percent). Only two of the four hospitals had contaminated disinfectant/antiseptic samples. All 24 isolates of Pseudomonas spp. tested were resistant to one or more of the 14 antimicrobial agents tested, with the prevalence of resistance to ciprofloxacin, norfloxacin, tobramycin, and gentamicin being 58.3 percent, 50.0 percent, 45.8 percent, and 41.7 percent, respectively. CONCLUSIONS: Our results show that contaminated disinfectants/antiseptics pose a health risk to patients, particularly in the pediatric and surgical wards. The high prevalence of resistance to antimicrobial agents exhibited by the Pseudomonas spp. that were isolated is of special therapeutic concern

Molecular analysis of salmonella anteritidis isolates from the Caribbean by pulsed-field gel electrophoresis / Análisis molecular de salmonella enteritidis aisladas en el Caribe mediante electroforesis en gel con pulsos eléctricos

Using pulsed-field gel electrophoresis (PFGE), between 1987 and 1996 we analyzed Salmonella enteritidis isolates from gastroenteritis cases in four Caribbean countries: Barbados, Saint Kitts and Nevis, Saint Lucia, and Trinidad and Tobago. We also determined the resistance of the isolates to 12 antimicrobial agents. Of the 129 isolates of S. enteritidis available for testing, DNA digested by XbaI revealed 13 distinctive PFGE patterns. The most prevalent XbaI PFGE patterns were group 1 (88 of 129 isolates, 68.2%) and group 2 (26 of 129, 20.2%). The patterns found among S. enteritidis isolates correlated with the geographical origin of the isolates. Of the 28 isolates from Barbados, 20 of them (71.4%) belonged to XbaI PFGE group 2, and of the 93 isolates from Trinidad and Tobago, 78 of them (83.9%) belonged to group 1. SpeI digestion of S. enteritidis genome was not as discriminatory as XbaI. Overall, of the 129 isolates, 67 of them (51.9%) exhibited resistance to one or more of the 12 antimicrobial agents that we tested. The prevalence of resistance was 53.8% for the S. enteritidis isolates tested from Trinidad and Tobago, 50.0% for those from Barbados, 28.6% for those from Saint Lucia, and 100.0% for one isolate from the island of Saint Kitts. Resistance was highest to triple sulfur (59 of 129 isolates, 45.7%), followed by furadantoin (10 of 129, 7.8%), ampicillin (7 of 129, 5.4%), and carbamycin (5 of 129, 3.9%)

Mediante electroforesis en gel con pulsos eléctricos (EGPE), se analizaron las Salmonella enteritidis aisladas entre 1987 y 1996 en casos de gastroenteritis de cuatro países caribeños: Barbados, Saint Kitts y Nevis, Santa Lucía y Trinidad y Tabago. También se determinó la resistencia de los aislados a 12 antibióticos. La digestión del ADN con la endonucleasa de restricción XbaI reveló 13 patrones distintos de EGPE entre los 129 aislados de S. enteritidis analizados; los más prevalentes fueron el grupo 1 (88 de 129; 68,2%) y el grupo 2 (26 de 129; 20,2%). Estos patrones se correlacionaron con el origen geográfico de los aislados. Así, de los 28 aislados de Barbados, 20 (71,4%) pertenecían al grupo 2, y de los 93 aislados de Trinidad y Tabago, 78 (83,9%) pertenecían al grupo 1. La digestión del genoma de S. enteritidis con la endonucleasa de restricción SpeI no fue tan discriminativa como la digestión con XbaI. En general, 67 de los 129 aislados (51,9%) mostraron resistencia a uno o más de los 12 antibióticos probados. La prevalencia de resistencia fue de 51% en los aislados de Trinidad y Tabago, de 50% en los de Barbados, de 28,6% en los de Santa Lucía y de 100% en el único aislado de la isla de Saint Kitts. La mayor resistencia correspondió a la triple sulfamida (sulfamerazina, sulfadiazina y sulfametazina: 59 de 129; 45,7%), seguida de la nitrofurantoína (10 de 129; 7,8%), la ampicilina (7 de 129; 5,4%) y la carbamicina (5 de 129; 3,9%).

Microbial quality of water in rural communities of Trinidad / Calidad microbiológica del agua en comunidades rurales de Trinidad

A cross-sectional study was conducted in four rural communities of northeastern Trinidad to determine the microbial quality of water supply to households and that quality's relationship to source and storage device. Of the 167 household water samples tested, total coliforms were detected in 132 of the samples (79.0 por cent), fecal coliforms in 102 (61.1 por cent), and E. coli in 111 (66.5 por cent). There were significant differences among the towns in the proportion of the samples contaminated with coliforms (P < 0.001) and E. coli (P < 0.001). Of 253 strains of E. coli studied, 4 (1.6 por cent) were mucoid, 9 (3.6 por cent) were hemolytic, and 37 (14.6 por cent) were nonsorbitol fermenters. Of 69 isolates of E. coli tested, 10 (14.5 por cent) were verocytotoxigenic. Twenty-eight (14.0 por cent) of 200 E. coli isolates tested belonged to enteropathogenic serogroups. Standpipe, the most common water source, was utilized by 57 (34.1 por cent) of the 167 households. Treated water (pipeborne in homes, tandpipes, or truckborne) was supplied to 119 households (71.3 por cent), while 48 households (28.7 por cent) used water from untreated sources (rain, river/stream, or well) as their primary water supply. The type of household storage device was associated with coliform contamination. Water stored in drums, barrels, or buckets was more likely to harbor fecal coliforms (74.2 por cent of samples) htan was water stored in tanks (53.3 por cent of samples), even after controlling for water source (P = 0.04). Compared with water from other sources, water piped into homes was significantly less likely to be contaminated with total coliforms (56.9 por cent) versus 88.8 por cent, P<0.001) and fecal coliforms (41.2 por cent versus 69.8 por cent, P<0.01), even when the type of storage device was taken into account. However, fecal contamination was not associated with whether the water came from a treated or untreated source. We concluded that the drinking water in rural communities in Trinidad was grossly unfit for human consumption, due both to contamination of vaious water sources and during household water storage

Se realizó un estudio transversal en cuatro comunidades rurales del nordeste de la isla de Trinidad para determinar la calidad microbiológica de los suministros domésticos de agua y la relación entre dicha calidad y el origen del agua y los recipientes utilizados para su almacenamiento. Entre las 167 muestras de agua doméstica analizadas, se detectaron coliformes totales en 132 (79,0%), coliformes fecales en 102 (61,1%) y Escherichia coli en 111 (66,5%). Hubo diferencias significativas entre los pueblos con respecto a la proporción de muestras contaminadas con coliformes (P < 0,001) y E. coli (P < 0,001). De las 253 cepas de E. coli estudiadas, 4 (1,6%) eran mucoides, 9 (3,6%) hemolíticas y 37 (14,6%) no fermentadoras de sorbitol. De 69 aislados de E. coli en los que se analizó esta característica, 10 (14,5%) fueron citotóxicos para las células Vero. Veintiocho de 200 (14,0%) aislados de E. coli analizados pertenecían a serogrupos enteropatógenos. El origen más frecuente del agua fueron las fuentes (57 de los 167 domicilios; 34,1%). Ciento diecinueve domicilios (71,3%) recibían agua tratada (agua canalizada hasta el domicilio, de fuente o de camión cisterna), mientras que los otros 48 (28,7%) utilizaban agua no tratada (de lluvia, ríos/arroyos o pozos) como principal suministro de agua. El tipo de recipiente de almacenamiento en el domicilio se asoció con la contaminación por coliformes. El agua almacenada en bidones, barriles o cubos tenía mayores probabilidades de albergar coliformes fecales (74,2% de las muestras) que el agua almacenada en tanques (53,3% de las muestras), incluso después de controlar el efecto del origen del agua (P = 0,04). En comparación con el agua de otras fuentes, el agua canalizada hasta las casas tenía una probabilidad significativamente menor de estar contaminada por coliformes totales (56,9% frente a 88,8%; P < 0,001) y coliformes fecales (41,2% frente a 69,8%, P < 0,01), incluso después de tener en cuenta el tipo de recipiente de almacenamiento. Sin embargo, la contaminación fecal no se asoció con el origen tratado o no tratado del agua. Concluimos que el agua para beber de las comunidades rurales de Trinidad era en general no apta para el consumo humano debido a su contaminación tanto en el origen como durante el almacenamiento en el domicilio