Human leukocyte antigen frequencies in a selected group of Lebanese Greek Orthodox

HLA classes I and II profiles were determined among 45 unrelated Lebanese greek orthodox by the complement dependent lymphomicrocytotoxicity assay. HLA epitope frequencies and alleles in linkage disequilibrium were determined; the obtained results were then compared to those reported for other groups. Moreover, possible HLA disease associations were examined; medical history in relation to diabetes, rheumatoid arthritis, and ankylosing spondilitis was taken for each of the 45 individuals. The results indicated that :1] there were similarities and differences in HLA frequencies and alleles in linkage disequilibrium in greek orthodox as compared to those in other groups. It is worth mentioning the higher frequencies of B35, DR11, and DQ3 and the existence of linkage disequilibrium between DR11 and DR52 and DR4 and DR53 in greek orthodox. 2] preliminary results indicate that there were no significant HLA disease associations between each of DR4 and rheumatoid arthritis, DR4 and insulin dependent diabetes mellitus [IDDM], and B27 and ankylosing spondilitis in the group studied. Such associations have been reported in north American Caucasians

SDS-page of mononuucl cell protein lysates of HLA - identical

The loci responsible for the most vigorous graft-rejection reactions are contained within the HLA complex in humans. However, even when a donor and recipient have identical HLA antigens, differences in minor histocompatibility loci outside the major histocompatibility complex [MHC] can also contribute to allograft rejection- This study dealt with the detection of differences other than HLA, between individuals, that may account for rejection of grafts. Mononuclear cell protein lysates were prepared following two different methods. Lysates from a pair of HLA-identical twins and from 10 HLA non-identical individuals were run on 12.5% reducing Sodium dodecyl sulphate SDS Polyacrylamide Gels. All Polyacrylamide Gels were silver stained. Mononuclear cell protein profiles were determined on the basis of the number of bands, their molecular weights and band intensity. HLA identical Individuals had the same mononuclear cell protein profiles. Slight differences in protein profiles were noted, however, between HLA non identical individuals. Observed differences were mainly confined to band intensities, which may indicate that some proteins may be over expressed in certain individuals. This over- expression may have important implications in graft rejection