ABSTRACT
Objective: To study the effects of Allium sativum extract [ASE] on blood lipoproteins and blood indices in Wistar albino rat model
Study design: Experimental study
Place and Duration of Study: This study was conducted at the Animal House, Isra University Hyderabad from September 2014 to June 2015
Materials and Methods: 80 albino rats were divided into 4 groups; Group 1- Controls [Placebo 0.9% isotonic saline], Group 2- ASE 100 mg/kg, Group 3- ASE 200 mg/kg and Group 4- ASE 300 mg/kg were given orally for 30 days. Blood sample was collected by cardiac puncture. Statistical analysis was performed on SPSS 22.0 by one way ANOVA and post Hoc Duncan test at 95% confidence interval
Results: Triglycerides, total cholesterol, LDLc and HDLc showed statistically significant differences among groups [p =0.0001]. High dose fed ASE showed significant reductions in TAG, TC and LDLc and a rise in HDLc. Also the blood indices showed improvement in ASE treated rats [p=0.001]
Conclusion: The Allium sativum extract reduces triglycerides, total cholesterol and low density lipoproteins and increases high density lipoprotein [HDLc]. Blood indices were also improved in high dose rats
ABSTRACT
Recognition of various gall stones in the patients attending surgical OPD by biochemical analysis of gall stones. Cross sectional study. ISRA Hospital, Hyderabad and Institute of Biochemistry Sindh University Jamshoro. Duration of study was 18 months. Cases were patients with gall stones who were diagnosed on ultrasonography. Gall stone analysis was done from Institute of Biochemistry Sindh University Jamshoro. A total of sixty nine subjects were included in the study. In the present study the mean Age was 35.81+ 8.12 years. Frequencies in different age groups are 15-24 [11.60%], 25-34 [24.64%], 35-44 [53.63%], 45-54 [8.69%], 55-64 [01.45%]. Age distribution is shown in Graph III-1. In the present study 49[71.0%] were females and 20[29%] were males with the female to male ration of 2.45:1. gender distribution is shown in Graph III-2. In the present frequencies of different types of gall stones was, cholesterol stones 38[55.07%], pigmented stones 20[28.99%] and mixed type of stones 11[15.94%]. Frequencies of different types of gall stones is shown in Graph III-3. In our study the frequency of cholesterol gall stone is more as compared to pigmented and mixed gall stones. A total of 55.07% of cholesterol gallstones were found in our study with the female predominance
ABSTRACT
Analysis and recognition of coagulopathies in patients with carcinoma of prostate by carrying out platelet count and D-dimers level. Case-control study. This study was conducted at ISRA Hospital, Hyderabad, Liaquat University of Medical and Health Sciences Hospital Hyderabad and NIMRA Jamshoro for a period of six months from from June 2011 to November 2011. Cases were patients with prostatic carcinoma who were diagnosed on biopsy examination. Controls were normal healthy randomly selected age matched adult males from Hyderabad without prostatic carcinoma. A total of one hundred subjects were included in the study. In Group I [Cases], the mean platelet count was 197.52 x 103 and in Group II [Controls], the mean platelet count was 286.06 x 103. In Group I [Cases], the mean D-dimers level was 0.692 mg/L and in Group II [Controls], the mean D-dimers level was 0.146 mg/L. Coagulopathies are frequently associated with prostate cancer and should be known to urologists and oncologists because they may compromise short-term prognosis and influence therapeutic strategies. Our results suggest that platelet count and D-dimer levels are altered in patients with prostate cancer
ABSTRACT
To observe the histological changes in gallbladder mucosa due to Cholelithiasis. Observational study. This study was carried out at Department of Surgery, Anatomy and Pathology of Isra University Hospital and Institute of Biochemistry Sindh University Jamshoro. Sixty nine cases of Cholelithiasis undergoing surgery were included in this study. After operation the gall bladders were obtained and fixed in 10% formalin. Gall bladder stone were obtained and their analysis was done in the Institute of Biochemistry Sindh University Jamshoro. Gross examination of gall bladders was done and for microscopy after hematoxylin and eosin staining, sections were obtained from the fundus, body and neck of the gallbladder. Additional sections were taken from grossly abnormal looking areas. Data was analyzed by using SPSS version 16.0. Chi square test was used to determine statistical significance between categorical groups [p<0.5]. It was observed that size of gall bladder were normal in 41 [59.4%], increased in 20 [29.0%] and shrunken in 08 [11.6%] cases. The mucosal changes seen in Cholelithiasis include, Cholestrolosis in 18 [26.08%], ulceration 07 [10.1%], atrophy 12 [17.4%] and lymphocytic infiltration in 67 [97%] cases. Cholelithiasis produced a series of histopathological changes in the gallbladder mucosa. These changes have no relation with type of gall stone