Correlation between timing of wounds and the level of soluble intercellular adhesion molecule type 1 [sICAM-1] in traumatized individuals

Cytokines are important in the control of wound healing. Many cytokines could be useful for the determination of wound age. The most important of these cytokines are the growth factors and adhesion molecules. Thirty traumatized individuals of both sexes were subjected to this study and were divided, according to the degree of injury severity score [ISS], into three groups; mild, moderate and severe trauma groups; each group consisted of ten patients. Each group is consisted of ten patients. Each group is further subdivided, according to sampling time, into three subgroups: within the 1[st] 24 hours of admission, on the third day, and on the fifth day of admission. The control group consisted of six persons. The present study was conducted to correlate between the level of the soluble intercellular adhesion molecule type-1 [sICAM-1] and the timing of wounds in traumatized individuals. The level of sICAM-1 was measured in plasma using the ELISA technique and was estimated in relation to the time passed since infliction of injury on the 1[st] day of admission, on the 3[rd] day and on the 5[th] day. A significant increase was noticed in all subgroups when compared with the control group. A characteristic pattern of sICAM-1 variation with the timing of wounds was at its maximum level on the 3[rd] day post-trauma and then decreases towards its normal level. A number of factors were also compared with the sICAM-1 level and these included the nature of injury, the associated severe head injuries, and the combing bone affection with the soft tissue injuries. All the previously mentioned factors in the present work did not affect the characteristics pattern of sICAM-1 variation with the timing of wounds. Furthermore, sICAM-1 level was found to be significantly correlated with the degree of injury severity. It is recommended to use sICAM-1in the estimation of the timing of wounds in traumatized individuals

matigated role of selective Cox.2 inhibitors against the early effects of accumulated doses of gamma-irradiation in mice

Clinical and experimental studies of the early and late effects of radiation on cells have led to optimization of radiotherapy schedules and to more precise modes of radiation delivery, radiation induced-injury on normal tissue can present either during or after the completion of radiotherapy. Early effects of small accumulated doses of gamma-irradiation in mice were studied at the present work. Fifty four mice were classified as the following negative control group I, distilled water subgroup Iia, etodolac subgroup IIb, subgroup IIIa was irradiated by accumulated dose of 0.5 Gy, subgroup IIIb received etodolac half an our pre-0.5 Gy irradiation, subgroup IVa was irradiated y accumulated dose of 1Gy, subgroup Ivb received etodolac pre-1 Gy irradiation, suroup Va was irradicated by accumulated dose of 2 Gy and the last subgroup Vb received etodolac pre-2 Gy irradiation. Analysis of bone marrow and jejunal cell cycle kinetic was carried out using flowcytometery and histochemical studies. The results revealed hat there was significant dose-dependant alternation of cell cycle kinetic of bone marrow and jejunal samples in the form of increase in GI and CVGI and decrease in percent SPE, percent G2 and PI. Mitotic delay, reduction in DNA content and chromatin condensation underneath the nuclear membrane of the cells in both cypts and villi of the jejunal sections could be also observed. Etodolac adminisration to the mice half an hour pre-irradiation could offer a great radioprotective effect. Research opportunities may help future clinical application of selective cyclooxygenase-2 inhibitors as chemopreventive drugs