effect of folic acid on the development of the dorsal surface of the tongue and evaluation of the role of epidermal growth factor and transforming growth factor-alpha

Nowaday women are planning to become pregnant are ad viced to increase their folic acid intake which is a water soluble vitamin B-complex group. Folic acid is essential for. cell division, tissue growth and it reduces the incidence of congenital abnormalities of embryo. The target of this study was to evaluate the histological effect of folic acid on the development of the dorsal surface of the tongue in pups of albino rat mothers fed a diet containing the optimum level of folic acid during pregnancy and lactation. The histological results were evaluated by light microscope and scanning electron microscope, also the roles of EGF and TGF alpha were evaluated. Conclusion: Administration of folic acid has a beneficial effect on the histological structure and development of the dorsal surface of the tongue

histological and ultrastructural study, on the effect of food flavours, [example monosodium glutamate] on oral epithelium in albino rats

This work was carried out to study the histological effect of monosodium glutamate [MSG] [a food flavor] on the oral epithelium in rats. The study included 15 adult albino male rats. They were divided into 2 groups: Group I of 5 rats as a control group and Group II of 10 rats as a study group. The study group was given a daily oral dose of 4 gm monosodium glulamate [MSG]/ Kg body weight for a period of one week followed by 2 gm/Kg body weight every 48 hours for 10 weeks. Then the animals were sacrificed and parts from the palatal giniva of both groups were prepared to be examined by light and electron microscopes. The histological results of the study group revealed disturbances in the thickness of the epithelium, together with hyaline degenerative changes in some cases. Histochemical examination revealed weak reaction of cells to [EGF-R and Cytokeratin pan], compared to their controls. Ultrastructural examination showed: altered ceil organelles, mitochondria decreased in number, distribution and number of tonofilaments were affected and condensed close to the nucleus. The nucleus was segregated clos faces in most cases surrounded by indistinct nuclear membrane. Chromatin was increased at the prephery in some cases. It was concluded that the use of MSG as a food flavor may lead to abnormal changes on oral epithelium. Therefore it is recommended to limit the uses of MSG as a food flavor. Abbreviations: EGF-R: epidermal growth factor. MSG: Monosodium glutamate

Histological and histochemical evaluation of the reaction of demineralized-freeze dried bone and med for porous polyethylene implant materials to bone during healing of osseous defects

The present study was performed to evaluate the histological reaction of two graft materials both are widely used clinically Demineralized freeze-drired bone [DFDBA] and Medpor on bone. They are different in chemical and physical composition. 15 rabbits [males] were used in this study. Osseous defects were created in the buccal side of their mandibles. Then the animals were divided into 3groups: group I [control], group II [study] that included osseous defects filled with DFDBA, group III [study] which included osseous defects filled with Medpor. The experimental animals were sacrificed in intervals 2,6,12 weeks. Histological and histochemical results revealed: successful bone healing in all groups. DFDBA group showed a number of inflammatory cell at the area of osseous defect after 2 weeks. Followed by formation of woven bone between the bone graft material then progressive bone formation, maturation and regeneration were observed. The mature bone was greater in amount than the two orther groups, fused completely to the host bone. The DFDBA was completely resorbed by the end of the experiment. The Med por group showed few inflammatory cells in 2 weeks then new bone formation and fibrous tissue in growth between its pores were observed together with areas of fusion to the host bone. By the end of the follow up period dense mature bone trabeculae attached to the Med por particles were forming a dense mass fused completely to the host bone. The control group showed normal bone healing less in amount than the DFDB group and less indense than the Med por group. Alkaline phosphatase enzyme activity was increased with the period of follow up marked on the surface of the host bone facing the graft materials and on the bony trabeculae between the materials particles of the both DFDB and Med por