ABSTRACT
Objective: determine the efficacy and safety of the non-cultured melanocyte-keratinocyte transplantation procedure in the treatment of stable resistant generalized vitiligo. Design: A simpler and modified method based on that of Olsson and Juhlin [1998] was performed this method uses a shaved biopsy skin sample one tenth the size of the recipient area. The skin sample is incubated, and the cells are mechanically separated using trypsin-EDTA solution and then centrifuged to prepare a suspension. Cell suspension is then applied to the derma braded depigmented skin area, and a collagen dressing is applied to keep it in place
Patients: twenty one patients with stable generalized vitiligo of different sites and sizes were treated with NMKT and followed up for 6 months
Intervention: Autologous, non-cultured melanocyte keratinocyte cell transplantation
Main Outcome Measure: initial pigmentation was observed. Regimentation was graded as excellent with 75 to 100% pigmentation, good with 50% to 74% fair with 25 to 49%, and poor with <25% pigmentation, assessment by modified vitiligo area scoring index, color match ,patient satisfaction and adverse events were assessed
Results: 9.66% showed excellent response, 11.50 % showed good response, 40.38% showed fair response and 38.46 % with poor response. Average percent change in VASI was 24.56% +-33.71. The color matching was excellent to good in 65.4% and poor in 34.6% of lesions, 3.8% of patients only were very satisfied and complications were minimal. Limitations: Limitations include small sample size, lack of control group and short follow-up period
Conclusions: NMKT is an easy economic technique, which may be used in resistant areas of stable vitiligo. The smaller the size of the lesion and the longer the stability duration the higher the percentage of regimentation response obtained. Results tend to be better over the trunk and proximal limbs than elbows, knees and distal extremities. Complications are minimal the most common is post inflammatory hyperpigmentation of the donor area
ABSTRACT
This study assessed the quality of life [QOL] and factors impaired among 165 HCV patients admitted to Zagazig University Hospitals. Data were collected via a questionnaire included the patients' socio demographic characteristics, medical history about HCV infection. A standard short form 36 questionnaire [SF36] was used to assess their quality of life. The results showed that all QOL domains were reduced, the most affected domain was the role limitation attributed to physical problems [88.5%], while the least affected one was the mental health domain [26.7%]. The study revealed that the patients' age negatively affected 3 domains [fatigue and tiredness, mental health and perception of pain]. The workers and male patients were affected in 3 domains [physical functioning, role limitation attributed to emotional problems and perception of pain] than others. QOL of highly educated patients were more impaired in physical functioning and role limitation attributed to physical and emotional problems. The patients with insufficient income recorded lower score in mental health domain only. The majority [78.8%] of the patients had bad QOL