New multicomponent spectrophotometric method for estimation of five hemoglobin derivatives in normal and traffic policemen

Several methods for the determination of five hemoglobin derivatives [oxy - carboxy - sulf - met - deoxy] hemoglobin based on multicomponent spectrophotometric analysis had been described. Most of these methods are applicable for the determination of the physiological concentration of both deoxy-Hb and oxy0Hb, and the oxygen saturation of blood samples. However, these methods suffered from some sources that were not easily overcome e.g. turbidity, interference due to pigments and negligible of other hemoglobin derivatives. The new technique has been applied in the simultaneous determination of SHb. Met-Hb, HbCO and the remaining functional Hb in the oxy-Hb form, with a conventional spectrophotometer. In this method, the measurements for the latter prepared extremely diluted solutions, with a light-path length 1cm. The 16 millimolar absorptivities of HbO[2], HbCO, Met-Hb and SHb, determined previously, at four wavelengths [500, 569, 577 and 620nm] that represent Met-Hb, HbCO, HbO[2], SHb respectively, were substituted into four linear equations of the type described by the theory of multicomponent spectrophotometric analysis with the four unknown concentrations of Hh pigments. This linear system of equations was solved by mathematical manipulation using the Gaussian elimination method for matrix calculation giving the unknown Hb derivatives concentrations

Using of angelica officinalis roots powder as a chelating agent of lead in rats

Lead poisoning still a dominant problem in all countries world wide. Treatment of lead poisoning is called chelation therapy. The aim of this work was to verify the chelation potency of angelica officinalis herb water extraction as it mentioned before in the Traditional Arabic Method. Results obtained by application of this extraction compared with those obtained from application of the most common chelator in the world [DMSA]. Results showed a strong chelation potency of the extraction against lead in blood, bone, liver and brain. Natural chelator was better that derived chemically hence no free radicals formed through chelation process, that verified by measuring the SOD and glutathione peroxidase activity. Groups received treatment without continuous exposure to lead gave better results than those received the treatment with continuous exposure to lead, i.e. it is very essential to avoid the toxicity test before starting the treatment courses

Human leukocytes antigens in insulin dependent diabetes mellitus [IDDM] type I

This study consists of 72 diabetic patients of type I and 52 diabetic patients of type II besides 35 normal control of matched age and sex. There are some alleles more frequent in IDDM group than in control. In patients, they are DQA1 *0301/2,*0102, *0101- DQBl*0201,*0302,*0602-3,*0301- DRB1*0101,*0301,*04,1101,. While in normal control,they are DQAI*0101,*0102,*103,*0201,*0501 DQBI*0301,*0602-3,*0201,*0601 ORB1*1101,*1501/2/3. Three times high positive percent were observed regarding rubella IgM of IDDM as compared to NIDDM while it was zero percent with respect to control

Superoxide dismutase and glutathione peroxidase activities in lead exposed workers

Superoxide dismutase [SOD] and glutathione peroxidase activities beside methemoglobin content and Met-Hb reductase system were investigated in workers of different blood lead concentration. The obtained data revealed high content of Met-Hb concentration with a different degrees of disorder in Met-Hb reductase. The auto-oxidation of HbO2 in lead exposed subjects resulted in the generation of superoxide radicals. This indicated high activities of SOD and GSH-Px with positive correlation of blood lead concentration

Bone marrow transplantation as a prophylactic of radiation injury

Bone marrow transplantation was used as prophylactic treatment of radiation injury at dose level of [6 Gy]. The resulting amelioration was studied on hemoglobin [Hb] after time intervals of transplantation 13 and 20 days. Hb content tended to increase gradually, and even became above normal after 21 days of transplantation. Not only the improvement occurred on quantitative concentration of Hb qualitative structure and function

Fate of incorporated 1,4-C 14 succinic acid in corn seedlings grown under saturated atmosphere of Co2 and normal conditions

Radioactivity distributions corn seedlings incubated in saturated atmosphere of CO2 -for 3 hours, and after transferring to normal condition for intervals 1,3 and 5 hours, after 2 C[14]alanine incorporation, was investigated. An increase in amino acids and sugars radioactivities was observed, with significant decrease in organic acids. Saturated atmosphere of Co2 leads to disorder in the function of kreb's cycle, which appeared as radioactivity accumulation in succinic acid and decrease the radioactivity of citric, malic, aconitic, and fumaric acids. Saturated atmosphere of Co2 affect in succinic acid dehydrogenase activity, which consequently lead to blok alanine oxidation to pynvie acid, and then through acids of kreb's cycle. Among amino acids, the radioactivity accumulation was detected in glutamic, alpha-amino butyric acid [10 folds], as compared to control. After 5 hours of transferring the seedlings to normal condition, the radioactivity of aspartic, asparagines, glutamic acids, and glycine + serine was returned to control level