Mechanism of Pinelliae Rhizoma-Citri Exocarpium Rubrum in Treatment of Metabolic Syndrome Based on Network Pharmacology / 中国实验方剂学杂志

Objective:By the method of network pharmacology， the mechanism of Pinelliae Rhizoma and Citri Exocarpium Rubrum in the treatment of metabolic syndrome was explored. Method:Effective components of Pinelliae Rhizoma and Citri Exocarpium Rubrum were retrieved by TCMSP database，and then selected by ADME parameters. TCMID，BATMAN-TCM，SymMap，TCM-MESH database were used to supplement effective components of TCMSP. TCMSP target prediction model was used to predict potential targets of drugs. DRUGBANK，DisGeNET，CTD，GeneCards，OMIM，PharmGkb，KEGG，DiGSeE databases were retrieved to obtain the targets of metabolic syndrome，and the chips were downloaded and analyzed through GEO database No.GSE98895 to screen out the differential genes of normal people and patients with metabolic syndrome，and supplement the target databases of metabolic syndrome. The intersections of Pinelliae Rhizoma-Citri Exocarpium Rubrum and metabolic syndrome disease targets were obtained by Rstudio 3.6.2. The above intersection targets were imported into the Metascape database for module analysis and overall GO（Biological Process），KEGG and Reactome pathway analysis. The core targets were selected from the intersection targets by using the cytohubba plug-in，the core genes were input into the database of BioGPS，Genecards to analyze the tissue distribution and subcellular distribution，and the core targets were assigned by using the database of DisGeNET. Result:A total of 34 active components and 120 targets of Pinelliae Rhizoma and Citri Exocarpium Rubrum were screened out，and 115 targets were obtained after the intersection of Rstudio 3.6.2. The results of Metascape module analysis and whole analysis were mostly the same，mainly involving the biological processes， such as ligand receptor interaction，calcium ion，cGMP-PKG，cholinergic synapse，thyroid hormone，insulin. The cytohubba plug-in was used to screen out 17 targets，involving 17 key genes， such as VEGFA and NOS3. The tissue and subcellular distribution of the core targets mainly included lymphoblasts，CD33+_myeloid cells，amygdala，pineal and cytoplasmic matrix，mitochondria. The main proteins were signal molecules，kinases and nucleic acids. Conclusion:Pinelliae Rhizoma and Citri Exocarpium Rubrum could treat metabolic syndrome through complex biological processes and pathways，such as blood circulation，ligand receptor interaction of nerve activity，cGMP-PKG，interleukin-related action，calcium ion. This indicates that traditional Chinese medicine（TCM） treated diseases through multi-component，multi-target，multi biological processes，multi-channel and other ways（which is also proved by the distribution of core genes in the tissue，subcellular and protein ascription information），indicating the superiority of the holism concept of TCM. Erchetang and its similar prescriptions are suitable for treating metabolic syndrome，which also indicates that the principle of "treating different diseases with the same therapy" of TCM is not only reflected at the theoretical level； and network pharmacology needs to be further proved in the combination with experimental verification.

Effect of the small needle knife through the Zusanli(ST 36) on behavior and hippocampal expression of NLRP3 and IL-1β in myalgia comorbid depressed rats / 中国骨伤

OBJECTIVE@#To observe the effect of the small needle knife through the Zusanli(ST 36) on behavior and hippocampal expression of NLRP3 and IL-1β in myalgia comorbid depressed rats.@*METHODS@#The rat models of myalgia comorbid depression were prepared by intraperitoneal injection of acute reserpine. Twenty-four SD male rats were randomly divided into control group, model group, small needle knife group and amitriptyline group, 6 rats in each group. The open field behavior and mechanical pain threshold of each group were detected. The thermal pain threshold was detected by intelligent hot plate test. The expression of NLRP3 and IL-1β in hippocampus of rats was detected by Western blotting.@*RESULTS@#Compared with the model group, the mechanical pain threshold of the foot was significantly improved in the small needle knife group (0.05). The expressions of NLRP3 and IL-1β in the hippocampus of the model group were significantly increased(0.05).@*CONCLUSIONS@#Small needle knife can improve the pathological state of myalgia comorbid depression caused by reserpine in rats. The mechanism may be related to the inhibition of NLRP3 inflammasome and IL-1β expression in central hippocampus.

Analysis of prognostic factors in early cervical adenocarcinoma / 中国实用妇科与产科杂志

OBJECTIVE: To investigate the prognostic factors of early stage adenocarcinoma of uterine cervix.METHODS: Totally 176 cases of early stage adenocarcinoma of uterine cervix who were admitted to Liaoning Cancer Hospital from January 2003 to May 2013 were analyzed retrospectively.RESULTS: The median age of 176 cases was 45(20-73),The 5-year overall survival rate was 81%. The 5-year tumor-free survival rate was 74.0%. The median survival time was 85 months.In univariate analysis,prognostic factors of patients with early cervical adenocarcinoma were clinical stage and lymph node metastasis.The five-year survival rate of ⅠB1,ⅠB2 and ⅡA1 was 86.0%,74.0% and 66.0%,and there were statistical differences(P<0.05).Compared with patients of ⅠB stage,the risk of death inⅡA1 patients was significantly increased(HR=2.92,95%CI 1.37-6.21).Totally 151 cases were pathologically proved with negative lymph node metastasis and 25 cases positive. The 5-year survival rates of them were 87.0% and 32.0% respectively and there were statistical differences between them(P<0.05).Multivariate analysis showed that only lymph node metastasis was an independent factor for survival(HR=5.86,95%CI 2.85-12.05).CONCLUSION: The 5-year survival rate of early-stage adenocarcinoma of uterine cervix is high;lymphnode metastasis is an important prognostic factor of early stage adenocarcinoma of uterine cervix.

Experimental study on malignant transformation of human bronchial epithelial cells induced by glycidyl methacrylate and analysis on its methylation / 生物医学与环境科学（英文）

<p><b>OBJECTIVE</b>To establish the model of human bronchial epithelial cells (16HBE) malignant transformation induced by glycidyl methacrylate (GMA) and define the different methylation genes at different stages.</p><p><b>METHODS</b>DNA was extracted at different 16HBE malignant phases and changes of genes DNA methylation at different stages were detected using Methylation chip of 'NimbleGen HG18 CpG Promoter Microarray Methylation'. Methylation-specific PCR (MSP) was used to observe the methylation status of some genes, and then compared with the control groups.</p><p><b>RESULTS</b>The result showed that GMA induced 16HBE morphorlogical transformation at the dose of 8 µg/mL, and cell exposed to GMA had 1374 genes in protophase, 825 genes in metaphase, 1149 genes in anaphase, respectively; 30 genes are all methylation in the 3 stages; 318 genes in protophase but not in metaphase and anaphase; 272 genes in metaphase but not in protophase and anaphase; 683 genes in anaphase but not in metaphase and protophase; 73 genes in protophase and metaphase but not in anaphase; 67 genes in protophase and anaphase but not in metaphase; 59 genes in metaphase and anaphase but not in protophase.</p><p><b>CONCLUSION</b>The pattern of DNA methylation could change in the process of 16HBE induced by GMA.</p>

Method for determining brodifacoum in workplace air by high-performance liquid chromatography / 中华劳动卫生职业病杂志

<p><b>OBJECTIVE</b>To establish a method for determining brodifacoum in workplace air by high-performance liquid chromatography (HPLC).</p><p><b>METHODS</b>Brodifacoum in workplace air was collected with a polytetrafluoroethylene filter and desorbed by mixed solution of methanol and dichloromethane (20:80, V:V), and was then separated using an ODS column and determined by an ultraviolet detector; retention time was used for identification, and peak area was used for quantification.</p><p><b>RESULTS</b>The concentration of brodifacoum showed a linear relationship with peak area within 0.2∼10.0 µg/ml; the elution efficiency was 91.6%∼95.1%; the detection limit was 0.08 µg/ml (injection volume: 20 µl eluate); the minimum detectable concentration was 0.000 67 mg/m(3) (calculated by 240 L air sample).</p><p><b>CONCLUSION</b>This HPLC method is convenient and simple for air collection and sample preparation and meets the methodological requirements. Therefore, this method can be used for the determination of brodifacoum in workplace air.</p>

Determination of brodifacoum in rat plasma by HPLC / 中华劳动卫生职业病杂志

<p><b>OBJECTIVE</b>A determination method of brodifacoum in rat plasma with bromadiolone as an internal standard was developed.</p><p><b>METHODS</b>A volume of 10 microl internal standard (bromadiolone) was added into rat plasma, and then extracted by 0.5 ml of acetonitrile by shaking for 2 min. The residue was dissolved with 200 microl of mobile phase after centrifugation for 10 min, and evaporation to dryness by Nitrogen blowing. A C18 column and PDA detector were used for separating and detecting. The wavelength was 254 nm, the flow rate was 1.0 ml/min, and the injection volume was 20 microl.</p><p><b>RESULTS</b>The liner range was 1.0-20 microg/ml, and the correlation coefficient was 0.9992. The detection limit was 0.3 microg/ml in plasma (S/N=3). The intra-assay and inter-assay coefficients of variation were 1.89%-2.45% and 2.51%-3.61% respectively. The recoveries in plasma at levels of low, middle and high concentrations were (80.8 +/- 3.1)%, (81.8 +/- 2.7)% and (87.9 +/- 3.6)% (n=6), respectively. The accuracies were 84.1%-91.5% and 86.7%-93.2%, respectively.</p><p><b>CONCLUSION</b>This method is simple, fast and accurate for the determination of brodifacoum in rat plasma.</p>

Methylation status of P16 gene during malignant transformation of human bronchial epithelial cells induced by glycidyl methacrylate / 中华劳动卫生职业病杂志

<p><b>OBJECTIVE</b>To analyze the methylation status of P16 gene at the different stages of malignant transformation of human bronchial epithelial cells (16HBE) induced by glycidyl methacrylate (GMA) and to explore the DNA methylation mechanisms.</p><p><b>METHODS</b>The cells exposed to GMA were harvested at the end of exposure (early stage), the 10th generation (protophase) and the 30th generation (anaphase), respectively. The methylation status of P16 promotor was detected by Methylation-specific PCR (MSP). The transformed 16HBE cells were compared with the normal 16HBE cells and the cells exposed to DMSO for methylation status.</p><p><b>RESULTS</b>At the early stage and protophase stage, the non-methylation status in P16 gene promotor of the normal 16HBE cells and the cells exposed to DMSO appeared, the methylation status in P16 gene promotor of the 16HBE cells exposed to GMA was detected to some extension. At the anaphase stage, the methylation status in P16 gene promotor of the 16HBE cells exposed to GMA or DMSO was detected to some extension.</p><p><b>CONCLUSION</b>Methylation status of P16 gene promoter was specific at the early stage and protophase stage of malignant transforming in 16HBE cells induced by GMA, which can serve as an early sensitive biological indicator for malignant transforming in 16HBE cells induced by GMA.</p>