In vitro susceptibility to fosfomycin in clinical and environmental extended-spectrum beta-lactamase producing and/or ciprofloxacin-non-susceptible Escherichia coli isolates

ABSTRACT Extended-spectrum beta-lactamase producing and ciprofloxacin-non-susceptible Escherichia coli are clinical and environmental issues. We evaluated the susceptibility profile of fosfomycin in non-susceptible E. coli isolated from urine and the environment. We measured the activity of fosfomycin against 319 and 36 E. coli strains from urine and environmental isolates, respectively, collected from rivers. Fosfomycin resistance profiles were investigated using the minimal inhibitory concentration (MIC), according to the Clinical and Laboratory Standards Institute (CLSI) and the European Committee for Antimicrobial Susceptibility Testing (EUCAST) guidelines. Antibiotic susceptibility testing revealed that 5% and 6.6% of urine samples were non-susceptible to fosfomycin according to CLSI and EUCAST guidelines, respectively. The fosfomycin MIC50/90 was 0.5/4 mg/L. Of the 36 E. coli isolates from river water, 11.1% and 13,8% were non-susceptible to fosfomycin according to CLSI and EUCAST, respectively (range ≤0.25 ≥512 mg/L). All the isolates with MIC ≥512 mg/L for fosfomycin showed the fosA3 gene. Fosfomycin resistance was more frequent in the environment than in clinical samples.

Resources for health literacy among caregivers of prematurely born children: a scoping review / Recursos para el alfabetismo en salud de cuidadores de niños nacidos prematuramente: revisión de alcance / Recursos para o letramento em saúde de cuidadores de crianças nascidas prematuras: revisão de escopo

ABSTRACT Objectives: to map the available evidence on resources used to promote health literacy among caregivers of prematurely born children during outpatient follow-up. Methods: the Joanna Briggs Institute's scope review protocol was utilized. The search encompassed six databases, incorporating studies from 2012 to 2022. Results: the three included publications revealed that the resources employed are: mobile applications, phone calls, individual counseling, videos, educational pamphlets, and group discussions. Implementing an education protocol during the transition home enhances scientifically grounded health promotion rates. Conclusions: there is limited literature addressing the health literacy of these caregivers. The nursing team plays a crucial role in health education and in developing resources applicable to these families.

RESUMEN Objetivos: mapear las evidencias disponibles sobre los recursos utilizados para la promoción del alfabetismo en salud de cuidadores de niños nacidos prematuramente en el seguimiento ambulatorio. Métodos: se utilizó el protocolo de revisión de alcance del Joanna Briggs Institute. La búsqueda se realizó en seis bases de datos, incluyendo estudios entre 2012 y 2022. Resultados: las tres publicaciones incluidas evidenciaron que los recursos utilizados son: aplicaciones para teléfonos móviles, llamadas telefónicas, asesoramiento individual, videos y folletos educativos, y discusiones en grupos. Implementar un protocolo educativo en la transición al hogar aumenta los índices de promoción de la salud respaldada científicamente. Conclusiones: hay poca información en la literatura sobre el alfabetismo en salud de estos cuidadores. El equipo de enfermería juega un papel fundamental en la educación en salud y en la creación de recursos que pueden aplicarse a estas familias.

RESUMO Objetivos: mapear as evidências disponíveis sobre os recursos utilizados para a promoção do letramento em saúde de cuidadores de crianças nascidas prematuras no seguimento ambulatorial. Métodos: utilizou-se o protocolo de revisão de escopo do Joanna Briggs Institute. A busca foi realizada em seis bases de dados, incluindo estudos entre 2012 e 2022. Resultados: as três publicações incluídas evidenciaram que os recursos utilizados são: aplicativos para celulares, ligações telefônicas, aconselhamento individual, vídeos e folhetos educativos e discussões em grupos. Efetivar um protocolo de educação na transição para casa aumenta os índices de promoção da saúde cientificamente embasada. Conclusões: pouco se aborda na literatura sobre o letramento em saúde desses cuidadores. A equipe de enfermagem desempenha um papel fundamental na educação em saúde e na construção de recursos que podem ser aplicados a essas famílias.

Color and surface roughness alterations of bulk-fill resin composites submitted to simulated toothbrushing with whitening dentifrices

Aim: To evaluate the surface roughness and color stability of bulk-fill resin composites after simulated toothbrushing with whitening dentifrices. The radioactive/relative dentin abrasion (RDA) and radioactive/relative enamel abrasion (REA) of dentifrices were also assessed. Methods: Specimens (n=10) of Tetric N Ceram Bulk Fill (TNCB), Filtek One Bulk Fill (FOB) resin composites, and Z100(Control) were prepared using a cylindrical Teflon matrix. Surface roughness (Ra, µm) was assessed by a roughness meter and the color evaluations (ΔEab , ΔE00 , WID ) were performed using a digital spectrophotometer based on the CIELAB system. Three measurements were performed per sample, before and after simulated toothbrushing with 3D Oral-B White Perfection (3DW) and Black is White (BW) dentifrices. The abrasivity (REA and RDA values) of the used dentifrices was also determined by the Hefferren abrasivity test. Results: The Ra values increased significantly in all resin composites after 3DW and BW toothbrushing. The acceptable threshold color varied among resin composites, and TNCB and Z100 presented the highest ΔEab and ΔE00 for BW dentifrice. The 3DW dentifrice was significantly more abrasive than BW dentifrice on enamel and dentin. Conclusions: simulated toothbrushing with tested whitening dentifrices increased the surface roughness at acceptable levels. The Tetric N Ceram Bulk-fill and Z100 composite showed the highest color alteration in BW. 3D White Perfection dentifrice was more abrasive on dentin and enamel than Black is White.

Outcomes and factors associated with tolerance in infants with non-IgE-mediated cow's milk allergy with gastrointestinal manifestations

Abstract Objectives To evaluate outcomes of oral food challenge (OFC) test to assess tolerance in infants with non-IgE-mediated cow's milk allergy (CMA) with gastrointestinal manifestations and explore clinical data predictive of these outcomes. Methods Single-center retrospective study including infants (age < 12 months) who were referred for CMA between 2000 and 2018 and underwent OFC on follow-up. A univariate logistic regression test was performed to evaluate variables associated with the outcomes of the follow-up OFC test. Results Eighty-two patients were included, 50% were male. Eighteen patients had a positive OFC test (22%). Most patients had presented with hematochezia (77%). The median age of symptom onset was 30 days. Two-thirds of the patients were on appropriate infant formula (extensively hydrolyzed or amino acid-based formula), exclusively or in association with breastfeeding. The median time on an elimination diet before the OFC test was 8 months (Q1 6 - Q3 11 months). All cases with positive follow-up OFC tests (n= 18) had been exposed to cow's milk-based formula before the first clinical manifestation of CMA. Five out of eight cases with Food Protein-Induced Enterocolitis Syndrome (FPIES) had positive OFC tests. Exposure to cow's milk-based formula before diagnosis, a history of other food allergies, hematochezia and diarrhea were predictors of a positive OFC test. Conclusions In infants with non-IgE-mediated CMPA with gastrointestinal manifestations, the use of cow's milk-based formula, a history of other food allergies, and hematochezia and diarrhea upon initial presentation were associated factors for the later achievement of tolerance.

Relationship between permanent upper anterior teeth and anthropometric facial measures in different populations: a narrative review

Aim: The main purpose of this study was to conduct a narrative review investigating the possible relationship between permanent maxillary anterior teeth and anthropometric facial parameters in different populations. Methods: Searches were performed in the PubMed, BVS (Biblioteca Virtual em Saúde) and SciELO databases to identify relevant scientific articles using the following search terms: "maxillary anterior teeth", "facial measurements" and "anthropometry", in such a way that 218 publications were found. After application of inclusion and exclusion criteria, 13 publications remained for full-text reading. Results: All studies involved male and female samples and it was notorious that male measurements were unanimously higher than those obtained in the opposite sex. The age of the analyzed participants ranged from 17 to 60 years; however, a mean age of 18 to 25 years was the most investigated in literature. In addition, facial measurements including the bizygomatic width, interpupillary distance, intercanthal distance, interalar width and intercommissural width have been proposed to help determine the correct size of anterior teeth. Conclusion: It was concluded that despite the limited number of studies, some factors that influence dental and facial dimensions, such as sex and age, can be identified. However, there is no standardization of the facial or dental parameters used in the studies, a fact that makes it difficult to establish a universal ratio for clinical dental practice

Psicometria brasileira da escala do medo da morte de Collett-Lester / Brazilian psychometrics of the Collett-Lester fear of death scale / Psicometría brasileña de la escala de miedo a la muerte de Collett-Lester

Resumo Estudo metodológico para avaliar a confiabilidade da escala de medo da morte de Collett-Lester e relacioná-la com a escala de bem-estar espiritual em amostra de 279 pessoas. A estrutura multifatorial da escala foi confirmada por quatro dimensões e 24 itens. A consistência interna obteve α de Cronbach: 0,951. A estabilidade foi confirmada pela correlação de Pearson (r=0,87 e p =0,000). A correlação escala de medo da morte de Collett-Lester ocorreu com três dos quatro fatores da escala de bem-estar espiritual, sendo positiva e variando de magnitudes fracas a moderadas. A escala de medo da morte de Collett-Lester se mostrou confiável à realidade brasileira.

Abstract Methodological study conducted with 279 individuals to evaluate the reliability of the Collett-Lester fear of death scale and relate it to the spiritual well-being scale. Its multifactorial structure was confirmed by four dimensions and 24 items. Internal consistency obtained Cronbach's α of 0.951. Pearson's correlation (r=0.87 and p =0.000) confirmed stability. Collett-Lester fear of death scale showed positive correlation with three of the four spiritual well-being scale items, ranging from weak to moderate magnitudes. The Collett-Lester fear of death scale proved to be reliable for the Brazilian reality.

Resumen Estudio metodológico que evalúa la confiabilidad de la escala de miedo a la muerte de Collett-Lester y la relaciona con la escala de bienestar espiritual en una muestra de 279 personas. La estructura multifactorial de la escala se confirmó en cuatro elementos y 24 ítems. La consistencia interna obtuvo el α de Cronbach de 0,951. La estabilidad se confirmó mediante la correlación de Pearson (r=0,87 y p =0,000). La correlación de la escala de miedo a la muerte de Collett-Lester ocurrió con tres de los cuatro factores de la escala de bienestar espiritual, siendo positiva y variando de magnitudes bajas a moderadas. La escala de miedo a la muerte de Collett-Lester es confiable al contexto brasileño.

As motivações da prática de atividade física durante a graduação de alunos de psicologia / The motivations for the practice of physical activity during the graduation of the psychology students

RESUMO O trabalho em questão, tem como objetivo central apresentar uma pesquisa voltada às principais motivações relacionadas à prática de atividades físicas durante o período de graduação no curso de Psicologia na Universidade Federal de Mato Grosso do Sul (UFMS). Desse modo, o instrumento utilizado será um questionário realizado e formulado pelos alunos pesquisadores, assim, pretende-se analisar o principal interesse precursor da prática, sendo as opções: diversão, hobby, descontração, competência, autoestima, saúde física ou influência social, além do levantamento da frequência da atividade realizada. Além disso, como fator secundário, procura-se perceber as razões daqueles que não realizam nenhum tipo de atividade, tendo como questões de escolha: motivos financeiros, falta de tempo, escolha própria e falta de motivação por parte da universidade. O questionário irá aborda ainda se os entrevistados pretendem iniciar alguma prática futuramente. Com base nos resultados, será possível deduzir a importância da prática de atividades físicas e principalmente seus benefícios, que se enquadram na melhora inclusive do desempenho acadêmico, e associar tais questões com os motivos dos não- praticantes também.

ABSTRACT The main objective of this work is to present a research focused on the main motivations related to the practice of physical activities during the graduation period in the Psychology course at the Federal University of Mato Grosso do Sul (UFMS). In this way, the instrument used will be a questionnaire carried out and formulated by the research students, thus, it is intended to analyze through this, the main precursor interest of the practice, being the options: fun, hobby, relaxation, competence, self-esteem, physical health or by social influence, and the frequency of the activity carried out. In addition, as a secondary factor, an attempt is made to understand the reasons of those who do not carry out any type of activity, having as choice issues: financial reasons, lack of time, own choice and lack of motivation on the part of the university, and as an additional, ask if they intend to start some practice in the future. Based on the results, it is possible to deduce the importance of the practice of physical activities and mainly its benefits, which fit in the improvement sometimes, even, of the academic performance, and to associate such questions with the motives of the non-practitioners as well.

Bond strength of bulk-fill resin composites: the effect of cavity preparation and aging

Aim: Evaluating the resin-dentin bond strength of Class II conventional and bulk-fill composite restorations, using different cavity sizes before and after aging. Methods: Seventy-five human molars were distributed into groups according to the buccolingual width of the cavities, conservative (n=25) and extended (n=50). They were divided according to the restorative material: conventional (Z100/control group) or bulk-fill resin composites (Filtek Bulk Fill/FBF; Tetric N Ceram Bulk Fill/TNCBF; Filtek Bulk Fill Flow/FBFF; Surefill SDR flow/SDR). The restored teeth were sectioned on sticks (n=50 per restorative materials + width cavities group), half were stored in Water/Ethanol 75% for 30 days and the other half were submitted to the immediate microtensile bond strength (µTBS) test. Data were analyzed applying the Three-Way Analysis of Variance (ANOVA), Bonferroni test, test t, and Weibull analyses (p<0.05). Results: SDR and FBF presented lower µTBSvalues for extended preparation when compared to the conservative preparation, before aging. After aging, only for the FBFF, a decrease in the µTBSvalues was observed. Comparing the µTBSvalues, before and after aging, the SDR demonstrated lower µTBSvalues after aging when the conservative cavity was used. A decrease in the µTBSvalues was observed for the Z100, the FBF and, the FBFF, after aging, when the extended cavity was used. Conclusion: The effect of cavity preparation and aging on the resin-dentin of Class II is material dependent. Most of the bulk-fill resin composites evaluated presented a similar performance to the conventional resin composites for all the conditions of this study

Evaluation of the Effectiveness of Cutting Scalpel Blades After Use and leaning by Different Methods / Avaliação da Efetividade do Corte de Lâminas de Bisturi Após Uso e Limpeza Por Diferentes Métodos

When performing incisions during oral and maxillofacial surgical procedures, the surgeon requires a blade that ensures precise cuts which reduce unnecessary tissue injuries. After searching the literature, we realized that there is a lack of studies that assesses scalpel blades for dental use. Herein, this study aims to assess the cutting power of new scalpel blades after cleaning with different methods. Sixty sterile scalpel blades were divided into seven groups: I) new blade; II) the second insertion; III) blade cleaned with gauze; IV) blade cleaned with gauze and saline; V) blade cleaned with saline; VI) blade cleaned with cotton, and VII) blade cleaned with cotton and saline. A universal testing machine (Osvaldo Filizola, São Paulo, Brazil) was used for the insertion and measurement of the shear strength. The results revealed that cleaning the blade with gauze and saline was the method that had the greatest loss of the cutting capability. The reinsertion of the scalpel blade without any type of cleaning or the cleaning of the blade with cotton and saline were the methods that obtained the best result, which did not compromise the mechanical properties of the tested material. This study concludes that cleaning methods affect the cutting power of scalpel blade

Na execução dos procedimentos cirúrgicos bucais e maxilofaciais o operador, ao realizar incisões, necessita de uma lâmina que garanta um corte preciso, diminuindo lesões desnecessárias aos tecidos. Ao fazer uma busca na literatura, constatou-se carência de estudos que avaliassem lâminas de bisturi de uso odontológico. Diante desse cenário, o objetivo do presente estudo foi avaliar o poder de corte de lâminas de bisturi novas e após limpeza com diferentes métodos. Sessenta lâminas de bisturi estéreis foram divididas em sete grupos: I) lâmina nova; II) segunda inserção; III) lâmina limpa com gaze; IV) lâmina limpa com gaze e soro; V) lâmina limpa com soro; VI) lâmina limpa com algodão e VII) lâmina limpa com algodão e soro. Para inserção e aferição da resistência ao corte utilizou-se uma máquina de ensaio universal (Osvaldo Filizola, São Paulo, Brasil). Os resultados revelaram que a limpeza da lâmina com gaze e soro fisiológico foi o método que teve maior perda de corte. Já a reinserção da lâmina de bisturi sem quaisquer tipos de limpeza ou a limpeza da lâmina com algodão e soro foram as que obtiveram melhor resultado, não comprometendo as propriedades mecânicas do material testado. Conclui-se, com a realização desse estudo, que os métodos de limpeza afetam o poder de corte das lâminas de bisturi.

Alterações na clínica odontológica decorrentes da Pandemia: a mínima intervenção como a primeira opção em Odontopediatria / Changes in the dental clinic resulting from the Pandemic: minimal intervention as the first option in Pediatric Dentistry

A pandemia pelo novo coronavírus impactou a prática clínica odontológica, trazendo a necessidade de consultas mais rápidas, por vezes remotas, e com diminuição na produção de aerossóis, com o intuito de diminuir a disseminação do vírus Sars-CoV-2. Desta maneira, as técnicas abrangidas pela filosofia da Mínima Intervenção (MI) ganharam destaque pois, além dos benefícios para o momento pandêmico atual, são técnicas pautadas em evidências científicas e que podem ser oferecidas com segurança e de maneira integral à saúde bucal dos pacientes. Neste comentário, nós retomamos como o odontopediatra pode agir de maneira minimamente invasiva desde o diagnóstico ao tratamento mais complexo, além de educar o paciente e envolver a família na mudança comportamental, compreendendo seu papel na conquista da saúde bucal. A odontologia de Mínima Intervenção oferece ao paciente, em qualquer idade ou etapa de desenvolvimento, uma abordagem na qual sua família compreende os riscos e benefícios do plano de tratamento e participa das decisões para a construção de uma nova realidade em saúde.

The pandemic caused by the new coronavirus has impacted clinical dental practice, bringing the need for faster consultations, sometimes remote, and with a decrease in the production of aerosols, in order to reduce the spread of the Sars-CoV-2 virus. In this way, the techniques covered by the philosophy of Minimum Intervention (MI) gained prominence because, in addition to the benefits for the current pandemic moment, they are techniques based on scientific evidence and that can be offered safely and in an integral way to the oral health of patients. In this commentary, we revisit how pediatric dentists can act in a minimally invasive way from diagnosis to more complex treatment, in addition to educating the patient and involving the family in behavioral change, understanding their role in achieving oral health. Minimal Intervention Dentistry offers patients, at any age or stage of development, an approach in which their family understands the risks and benefits of the treatment plan and participates in decisions to build a new health reality.

Doppler Ultrasound of the Umbilical Artery: Clinical Application / Ultrassonografia Doppler da artéria umbilical: Aplicação clínica

Abstract Objective To provide a survey of relevant literature on umbilical artery Doppler ultrasound use in clinical practice, technical considerations and limitations, and future perspectives. Methods Literature searches were conducted in PubMed and Medline, restricted to articles written in English. Additionally, the references of all analyzed studies were searched to obtain necessary information. Results The use of this technique as a routine surveillance method is only recommended for high-risk pregnancies with impaired placentation. Meta-analyses of randomized trials have established that obstetric management guided by umbilical artery Doppler findings can improve perinatal mortality and morbidity. The values of the indices of Umbilical artery Doppler decrease with advancing gestational age; however, a lack of consensus on reference ranges prevails. Conclusion Important clinical decisions are based on the information obtained with umbilical artery Doppler ultrasound. Future efforts in research are imperative to overcome the current limitations of the technique.

Resumo Objetivo Compilar informação relevante proveniente da literatura atual sobre a ultrassonografia Doppler das artérias umbilicais (AUs) na prática clínica, considerações e limitações técnicas e perspectivas futuras. Métodos A pesquisa bibliográfica foi realizada nos bancos de dados PubMed e Medline e restringiu-se a artigos escritos na língua inglesa. Recorreu-se também à bibliografia dos artigos selecionados, quando necessário, para obter informação relevante. Resultados A utilização desta técnica como método de vigilância de rotina está apenas recomendada emgravidezes de alto risco comdisfunção placentar.Metanálises de estudos randomizados mostraram que o seguimento obstétrico baseado nos achados do Doppler da artéria umbilical pode melhorar a mortalidade e a morbilidade perinatal. É consensual que os valores dos índices Doppler da AU decrescem com o avanço da idade gestacional. No entanto, há ainda muita incerteza quanto aos valores de referência. Conclusão As informações obtidas através da AU Doppler US são a base para muitas decisões clínicas importantes. Trabalhos de investigação nesta área são essenciais para tentar colmatar atuais limitações da técnica.

Avaliação in vitro e in silico da atividade do S-(-)-Álcool Perílico sobre linhagens celulares do Carcinoma Epidermóide de Língua / In vitro and in silico evaluation of the activity of S-(-)-Peryllic Alcohol on Tongue Squamous Carcinoma cell lines

O carcinoma epidermóide oral (CEO) é a neoplasia maligna mais frequente da cavidade oral e constitui um problema de saúde pública devido a sua alta taxa de incidência e mortalidade devido em muitos casos ao fracasso terapêutico e a resistência tumoral. Assim sendo, destaca-se a busca por novas moléculas biologicamente ativas, como as encontradas nos produtos de origem natural. Este trabalho tem como objetivo avaliar a atividade antineoplásica do S-(-)-álcool perílico (POH) em culturas de células de CEO de língua e predizer sua afinidade através de modelo computacional sobre proteínas que regulam o ciclo celular. Para isso, foram utilizadas duas linhagens celulares de CEO de língua, HSC-3 e SCC-25. Os seguintes grupos foram analisados: G0 (controle; células cultivadas na ausência de POH), G1 (células tratadas com cisplatina a 40 µM), G2 (células tratadas com POH a 0,5 mM), G3 (células tratadas com POH a 1,0 mM), G4 (células tratadas com POH a 1,5 mM) e G5 (células tratadas com POH a 3,0 mM). Diferenças entre estes grupos foram investigadas através dos seguintes ensaios: viabilidade celular (Alamar Blue e Live/Dead assay) e atividade migratória (Wound healing). Foi também realizada a predição de afinidade entre o POH e as moléculas de controle do ciclo celular utilizando a docagem molecular com emprego do software Molegro Virtual Docker, v. 6.0.1. Os dados foram tratados estatisticamente pelo GraphPad Prism 6.0 (GraphPad Software, EUA), análises paramétricas utilizando teste Anova, pós-teste de Tukey e teste estatístico não-paramétricos de Kruskal-Wallis, seguido pelo teste t de estudent foram adotados para determinação de diferenças entre os grupos experimentais. O índice de significância considerado neste trabalho foi de 5%. Para ambas as técnicas de avaliação da viabilidade celular (Alamar Blue e Live/dead assay) analisadas neste trabalho, o POH foi capaz de reduzir a viabilidade celular de linhagens do CEO de língua de maneira dosedependente e tempo-dependente (p<0,05). As concentrações de 1,5 mM e 3 mM do POH obtiveram resultados melhores ou semelhantes aos encontrados na cisplatina 40 µM, para as duas linhagens, na avaliação da viabilidade celular (p<0,05). Os valores de IC50 do POH foram de 1,5 mM para a célula SCC-25 em todos os intervalos de tempo (24 h, 48 h e 72 h), uma vez que, para a linhagem HSC-3, foram de 3 mM para os tempos de 24 h e 48 h e de 1,5 mM para o intervalo de 72 h. O POH foi capaz de inibir a migração das duas linhagens celulares de CEO de maneira dependente da concentração (p≤0,05), comparados ao grupo controle. A habilidade da molécula POH se ligar a proteínas responsáveis pela ativação do ciclo celular foi avaliada usando docking models. Dentre elas, a proteína GTPase Kras mostrou a melhor energia de ligação (-86.70 kcal/mol), apresentando ligações de hidrogênio com os resíduos THR58 (A) e ASP57 (A) e ligações estéricas com os resíduos TRY32 (A) e ALA18 (A). As evidências deste estudo corroboram a ideia de que o POH possui atividade sobre o CEO, sugerindo que essa molécula possa ser uma forte candidata para o desenvolvimento de medicamentos direcionados ao tratamento desta patologia (AU).

Oral squamous cell carcinoma (OSCC) is the most frequent malignant neoplasm of the oral cavity and constitutes a public health problem due to its high incidence and mortality rate caused in many cases by therapeutic failure and tumor resistance. Therefore, the search for new biologically active molecules stands out, such as those found in products of natural origin. This work aims to evaluate the antineoplastic activity of S-(-)-perillyl alcohol (POH) in cell cultures of tongue CEO and to predict its affinity through a computer model on proteins that regulate the cell cycle. For this purpose, two cell lines of tongue CEO were used, HSC-3 and SCC-25. The following groups were analyzed: G0 (control; cells cultured in the absence of POH), G1 (cells treated with 40 µM cisplatin), G2 (cells treated with 0.5 mM POH), G3 (cells treated with 1 .0 mM), G4 (cells treated with 1.5 mM POH) and G5 (cells treated with 3.0 mM POH). Differences between these groups were investigated through the following assays: cell viability (Alamar Blue and Live/Dead assay) and migratory activity (Wound healing). Affinity prediction between POH and cell cycle control molecules were also performed using molecular docking using Molegro Virtual Docker, v. 6.0.1. The data was statistically treated by GraphPad Prism 6.0 (GraphPad Software, USA), parametric analysis using Anova test, Tukey post-test and Kruskal-Wallis non-parametric statistical test, followed by t student test were adopted for determination of differences between the experimental groups. The significance index considered in this work was 5%. For both cell viability assessment techniques (Alamar Blue and Live/dead assay) analyzed in this work, POH was able to reduce the cell viability of tongue CEO lines in a dose-dependent and time-dependent manner (p<0 .05). The concentrations of 1.5 mM and 3 mM of POH obtained better or similar results to those found in 40 µM cisplatin, for the two strains, in the evaluation of cell viability (p<0.05). The IC50 values of POH were 1.5 mM for the SCC-25 cell at all time intervals (24 h, 48 h and 72 h), since for the HSC-3 line they were 3 mM for 24 h and 48 h times and 1.5 mM for the 72 h interval. POH was able to inhibit the migration of the two DSC cell lines in a concentration-dependent manner (p≤0.05), compared to the control group. The ability of the POH molecule to bind to proteins responsible for cell cycle activation was evaluated using docking models. Among them, the protein GTPase Kras showed the best binding energy (-86.70 kcal/mol), featuring hydrogen bonds with residues THR58 (A) and ASP57 (A) and steric bonds with residues TRY32 (A) and ALA18 ( THE). The evidence from this study supports the idea that POH has antineoplastic activity on the CEO, suggesting that this molecule may be a strong candidate for the development of drugs aimed at the treatment of this pathology (AU).

Multiple complex odontomas of the mandible: a rare case report and literature review / Múltiplos odontomas complexos em mandíbula: relato de um caso raro e revisão da literatura / Múltiples odontomas complejos de la mandíbula: reporte de un caso raro y revisión de la literatura

Abstract Odontomas are the most common tumor-like (hamartoma) odontogenic lesions in the gnathic bones. There are two main types: complex odontoma which is composed mainly of amorphous calcification, and compound odontoma which is characterized by irregular tooth-like structures. Odontomas are generally asymptomatic and in most cases involve only one quadrant of the maxilla or mandible. The treatment of choice is surgical removal. The aim of the present study is to report a rare case of multiple complex odontomas affecting the mandible and to review the literature on the clinical and pathological features of these tumors.

Resumo Odontomas são as lesões odontogênicas mais comuns nos ossos gnaticos mais comuns. Existem dois tipos principais: odontoma complexo que é composto principalmente de calcificação amorfa, e odontoma composto que é caracterizado por estruturas irregulares semelhantes à dentes. Odontomas são geralmente assintomáticos e, na maioria dos casos, envolvem apenas um quadrante da maxila ou mandíbula. O tratamento escolhido é a remoção cirúrgica.

Resumen Los odontomas son las lesiones odontogénicas más comunes en los huesos gnáticos más comunes. Hay dos tipos principales: odontoma complejo, que se compone principalmente de calcificación amorfa, y odontoma compuesto, que se caracteriza por estructuras irregulares similares a dientes. Los odontomas suelen ser asintomáticos y, en la mayoría de los casos, afectan solo un cuadrante del maxilar o la mandíbula. El tratamiento elegido es la extirpación quirúrgica.

Spiders in Brazil: from arachnidism to potential therapeutic use of their venom part 1 of 2

Spider envenomation, generically known as arachnidism, is described in many places around the world. In terms of medical importance, the following genera of animals stand out as the main origin of the morbid condition: Atrax (Sidney funnel web spider), Steatoda (false black widow), Latrodectus (black widow), Loxosceles (brown spider) and Phoneutria (armed spider), the last three causing accidents involving spiders in Brazil. This article, part 1 of 2, aims to present the main aspects of arachnidism in the country, with an emphasis on the biology and geographical distribution of spiders, biochemistry of the venom, pathogenesis and epidemiology of arachnidism, as well as prevention of the morbid condition.

Pindborg tumor associated with a supernumerary tooth: a case report

The calcifying epithelial odontogenic tumor is a rare benign neoplasm that accounts for approximately 1% of all odontogenic tumors. Most of the cases occur in the posterior mandible, and a few involve the maxilla. Despite their relatively indolent biological behavior, tumors in the maxilla tend to grow fast. We report the case of a 33-year-old female patient exhibiting swelling in the right maxilla. An isodense area associated with an impacted supernumerary tooth was found on imaging examination. The histopathologic diagnosis was a calcifying epithelial odontogenic tumor. The treatment of choice was surgical removal of the lesion and associated dental elements. The patient has been followed up for 11 months and shows no signs of recurrence. Besides describing this case, we reviewed the literature on the association of calcifying epithelial odontogenic tumors with supernumerary teeth and found two case reports addressing this subject.

Relationship between power and knowledge in choosing a cesarean section: women's perspectives / Relaciones de poder y saber al escoger por el parto cesárea: perspectiva de las puérperas / Relações de poder e saber na escolha pela cesárea: perspectiva de puérperas

ABSTRACT Objectives: to analyze the reasons and motives that initiates the decision to choose a cesarean section, by describing the plots, power relationships, struggles, and systems of truths regarding this method of childbirth. Methods: a qualitative study, inserted into a poststructuralist perspective, and conducted in a city in southern Rio Grande do Sul state. The settings were a teaching hospital and home residences. Thirteen postpartum women who had a cesarean section participated. The analysis consisted of questions based on the information produced and articulated with Foucauldian theories. Results: two categories were developed: "I wanted a vaginal delivery, but it was a cesarean section", and, "Are you sure you want a vaginal delivery: paths and detours in the choice of cesarean section". Final Considerations: the study allowed us to identify problems in the choice for a cesarean section, which is associated with the circulation of "truths" that occur via discourses on society.

RESUMEN Objetivos: analizar las razones y motivos que llevaron a escoger el parto cesáreo, utilizando la descripción de tramas, relaciones de poder, luchas y régimen de verdades sobre esa forma de parir. Métodos: investigación cualitativa, incluida en la vertiente postestructuralista y realizada en un municipio del sur de Rio Grande do Sul. Tuvo como escenarios, un hospital docente y los domicilios. Participaron 13 puérperas sometidas a parto cesárea. El análisis, fue construido por preguntas elaboradas frente a las informaciones producidas y articuladas con las teorías Foucaultianas. Resultados: fueron elaboradas dos categorías: ¿yo quería parto vaginal, pero fue cesaría y tú tienes seguridad que quieres parto normal?: trayectoria y obstáculos para decidir por el parto cesárea. Consideraciones Finales: el estudio permitió problematizar que escoger la cesaría está condicionada a un círculo de "verdades", que ocurren por medio de discursos en la sociedad.

RESUMO Objetivos: analisar as razões e motivos que mobilizam a escolha pela cesariana por meio da descrição das tramas, relações de poder, lutas e regimes de verdades sobre essa forma de parir. Métodos: pesquisa qualitativa, inserida na vertente pós-estruturalista e realizada em um município do sul do Rio Grande do Sul. Teve como cenários um hospital de ensino e os domicílios. Participaram 13 puérperas que realizaram cesariana. A análise se constituiu de questões elaboradas diante das informações produzidas e articuladas com as teorizações foucaultianas. Resultados: foram elaboradas duas categorias: Eu queria parto vaginal, mas foi cesárea, e Tu tens certeza que queres parto normal?: caminhos e descaminhos na escolha pela cesariana. Considerações Finais: o estudo permitiu problematizar que a escolha pela cesariana está veiculada à circulação de "verdades", que acontecem por meio de discursos na sociedade.

Effectiveness of Chewing Gum Containing CPP-ACP for Remineralisation of Demineralised Enamel around Orthodontic Brackets: An in Situ Study

Abstract Objective: To evaluate the effectiveness of chewing gums containing CPP-ACP in remineralisation of demineralised enamel around orthodontic brackets. Material and Methods: Enamel blocks from 120 bovine incisors were used. The blocks were flattened and polished, followed by the development of incipient subsurface caries. The blocks have been subjected to a baseline microhardness analysis. Orthodontic brackets were bonded to the exposed area of the blocks, reserving an area for hardness analysis. An in situ crossover (three-way crossover phases of 21 days with an interval of one week between them), controlled, randomised and blind experimental model was used, with the participation of 12 volunteers divided into groups: G1 - negative control group, without chewing gum; G2 - conventional sugar-free chewing gum, without CPP-ACP (placebo); and G3 - sugar-free chewing gum with CPP-ACP. The following parameters were analysed: superficial linear (Ra), volumetric roughness (Sa), and superficial structural loss, which was indicated by Gap measurement (difference between the healthy and treated area). Statistical tests used were the Friedman, Wilcoxon and Shapiro-Wilk, Kruskal Wallis' nonparametric tests and ANOVA. Results: There was a significant reduction in enamel microhardness after demineralisation in all the groups (p<0.05). This deleterious effect was attenuated in the three groups after the final evaluation, although there were no statistical differences (p<0.05); however, the final values did not return to the baseline values (p<0.05). Conclusion: Chewing gum containing CPP-ACP did not promote in situ remineralisation of demineralised enamel around orthodontic brackets.

Comparison of two different biomaterials in the bone regeneration (15, 30 and 60 days) of critical defects in rats

ABSTRACT Purpose To evaluate and compare two types of different scaffolds in critical bone defects in rats. Methods Seventy male Wistar rats (280 ± 20 grams) divided into three groups: control group (CG), untreated animals; biomaterial group 1 (BG1), animals that received the scaffold implanted hydroxyapatite (HA)/poly(lactic-co-glycolic) acid (PLGA); and biomaterial group 2 (BG2), animals that received the scaffolds HA/PLGA/Bleed. The critical bone defect was induced in the medial region of the skull calotte with the aid of an 8-mm-diameter trephine drill. The biomaterial was implanted in the form of 1.5 mm thick scaffolds, and samples were collected after 15, 30 and 60 days. Non-parametric Mann-Whitney test was used, with the significance level of 5% (p ≤ 0.05). Results Histology revealed morphological and structural differences of the neoformed tissue between the experimental groups. Collagen-1 (Col-1) findings are consistent with the histological ones, in which BG2 presented the highest amount of fibers in its tissue matrix in all evaluated periods. In contrast, the results of receptor activator of nuclear factor kappa-Β ligand (Rank-L) immunoexpression were higher in BG2 in the periods of 30 and 60 days, indicating an increase of the degradation of the biomaterial and the remodeling activity of the bone. Conclusions The properties of the HA/PLGA/Bleed scaffold were superior when compared to the scaffold composed only by HA/PLGA.

Crescimento bacteriano nas mãos dos profissionais de saúde: implicações na prevenção de infecções hospitalares / Bacterial growth on the hands of health care workers: implications for preventing nosocomial infections

RESUMO Objetivo analisar o crescimento bacteriano em amostras coletadas das mãos de profissionais de saúde após higiene com água e sabão. Métodos estudo transversal analítico, realizado com profissionais de saúde por meio da coleta de amostras (print) das polpas digitais da mão dominante, em placas contendo meio de cultura cromogênico, para análise microbiológica em relação à presença das colônias. Utilizaram-se o Teste de Fisher e Qui-quadrado. Resultados foram coletadas 73 amostras e 67 (91,8%) apresentaram crescimento bacteriano. O Staphylococcus aureus metilcilina resistente foi detectado em 19 (26,0%). Destaca-se associação significativa entre colônias com perfil de multirresistência e o tempo de atuação (p=0,030) e profissão (p=0,041). Conclusão houve crescimento bacteriano nas amostras após higiene das mãos, com maior crescimento de bactérias multirresistentes entre profissionais de enfermagem e àqueles com maior tempo de atuação. Estes resultados podem contribuir para detectar as lacunas acerca das medidas adotadas para a prevenção de infeções.

ABSTRACT Objective to analyze bacterial growth in samples collected from the hands of health professionals after hygiene with soap and water. Methods cross-sectional analytical study, carried out with healthcare professionals by collecting samples (print) from the digital pulp of the dominant hand on plates containing chromogenic culture medium for microbiological analysis regarding the presence of colonies. Fisher's and Chi-square tests were used. Results 73 samples were collected and 67 (91.8%) showed bacterial growth. Methicillin-resistant Staphylococcus aureus was detected in 19 (26.0%). A significant association between colonies with multidrug-resistance profile and the time of performance (p=0.030) and profession (p=0.041) was highlighted. Conclusion there was bacterial growth in samples after hand hygiene, with higher growth of multidrug-resistant bacteria among nursing professionals and those with longer time of work. These results may contribute to detect the gaps about the measures adopted for infection prevention.