ABSTRACT
Background: Nonsyndromic cleft lip and/or palate [NSCL/P] is the most common orofacial birth defect, often attributed to ethnic and environmental differences. Up to now, linkage analyses and genome-wide association studies have identified several genomic susceptibility regions for NSCL/P. The WNT genes including WNT3 are strong candidates for NSCL/P, since they are involved in regulating mid-face development and upper lip fusion. This study tested association of the WNT3 polymorphisms, rs- 3809857 G/T and rs9890413 G/A, with the risk of NSCL/P in a population of Iranian infants
Methods: The allelic and genotypic frequencies for each participant were determined in 113 unrelated Iranian subjects with NSCL/P and 220 control subjects using PCR and restriction fragment length polymorphism [RFLP] methods. A p-value of
Results: The WNT3 rs3809857 GT genotype was significantly lower [p=0.039, OR=0.55, 95% CI=0.30-0.97] in the NSCL/P [21.2%] than the control group [30.42%]. For the WNT3 rs9890413 G/A polymorphism, neither genotype nor allele frequencies were significantly different between the case and control groups
Conclusion: Our results indicated that the WNT3 rs3809857 GT genotype may have a protective effect against NSCL/P in Iranian population
ABSTRACT
Background: Orofacial cleft is the most common congenital defect of the maxillofacial region. Its non-syndromic type is multi-factorial, and several genes are involved in its occurrence. This study aimed to assess the interaction effect of Rsal and BamHI polymorphisms of Transforming Growth Factor-alpha [TGFa] gene and Bone Morphogenetic Protein-2 [BMP2] and BMP4 variants on the occurrence of Non-Syndromic Cleft Lip and Palate [NSCLP] in the Iranian population
Methods: This case-control study was conducted on 120 children with NSCLP and 215 healthy children. Genotyping of the TGFA/BamHI [rs11466297], TGFA/RsaI [rs3732248], BMP4 [rs17563] and BMP2 [rs235768] was performed by Polymerase Chain Reaction [PCR] and Restriction Fragment Length Polymorphism [RFLP] methods. Logistic regression was applied to determine the effective factors and the interaction effect of different variants on the occurrence of NSCLP
Results: Gender of patients had no significant association with the occurrence of NSCLP [p=0.335]. Multiple logistic regression showed that the interaction effect of the aforementioned polymorphisms on the occurrence of NSCLP was not statistically significant [p=1.000]
Conclusion: Although the individual effect of each of the BMP4, BMP2, RsaI and BamHI variants on the occurrence of NSCLP in the Iranian population has been previously confirmed, their interaction does not play a role in this respect
ABSTRACT
Introduction: Myofascial pain syndrome [MPS] treatment is challenging with a high recurrence rate and still lacks a clear treatment frame. Therefore research on new, more efficient and long lasting effect treatment modalities is necessary. This study looked at the effects of intravenous laser therapy [IVL] and percutaneous low level laser [PLLL] in the management of shoulder MPS
Methods: In this randomized controlled trial, 30 patients fulfilling inclusion criteria were randomly equally allocated to 3 groups, control, IVL and PLLL. Control group received 12 sessions of placebo low level laser, IVL group received 12 sessions of IVL therapy, and PLLL group received 12 sessions of PLLL therapy. All patients were trained for better body posture, body mechanics, gentle massage of trigger points, stretching exercises of affected muscle [trapezius], and received 10 mg of oral nortriptyline regimen every night for 3 months. Outcomes included pain severity, functional disability, and quality of life. Patients were assessed using Numeric Rating Scale [MRS], Pain Disability Index [PDI], and Short Form Health Survey [SF-12]. Data collected were analyzed using analysis of variance [ANOVA], Mann-Whitney and f tests
Results: The mean of PDI and maximum pain intensity during day and night significantly reduced in both PLLL and IVL groups compared to control group. Although pain severity and PDI reduction was more pronounced in IVL group compared to PLLL group, the differences were not statistically significant. Also, quality of life statistically significantly improved in both IVL and PLLL groups compared to control group was more, and although higher in IVL group, the difference was not statistically significant when compared to PLLL group. No side effects were observed in the intervention groups
Conclusion: Intravenous laser and PLLL therapy had a positive effect on pain severity and PDI reduction, and quality of life in this study. Also no adverse event was recorded. Thus, intravenous lasers and PLLL therapy seem to be effective complementary modalities in managing patients with shoulder MPS
Subject(s)
Humans , Male , Female , Adult , Middle Aged , Laser Therapy , Low-Level Light TherapyABSTRACT
Cleft lip with or without cleft palate [CL/P] is one of the most common congenital anomalies and the etiology of orofacial clefts is multifactorial. Transforming growth factor alpha [TGFA] is expressed at the medial edge epithelium of fusing palatal shelves during craniofacial development. In this study, the association of two important TGFA gene polymorphisms, BamHI [rs11466297] and RsaI [rs3732248], with CL/P was evaluated in an Iranian population. The frequencies of BamHI and RsaI variations were determined in 105 unrelated Iranian subjects with nonsyndromic CL/P and 218 control subjects using PCR and RFLP methods, and the results were compared with healthy controls. A p-value of <0.05 was considered statistically significant. The BamHI AC genotype was significantly higher [p=0.016] in the patients [12.4%] than the control group [5.0%]. The BamHI C allele was significantly higher [p=0.001; OR=3.4, 95% CI: 1.6-7.4] in the cases [8.0%] compared with the control group [2.5%]. Our study showed that there was an association between the TGFA BamHI variation and nonsyndromic CL/P in Iranian population
ABSTRACT
Intravenous laser blood irradiation was first introduced into therapy by the Soviet scientists EN.Meschalkin and VS.Sergiewski in 1981. Originally this method was developed for the treatment of cardiovascular diseases. Improvement of rheologic properties of the blood as well as improvement of microcirculation and reduction of the area of infarction has been proved. Further, reduction of dysrhythmia and sudden cardiac death was achieved. At first, only the Helium-Neon laser [632.8 nm] was used in this therapy. For that, a power of 1-3mW and a period of exposure of 20-60 minutes were applied. The treatments were carried out once or twice a day up to ten appointments in all1. In the years after, many, and for the most part Russian studies showed that helium-neon laser had various effects on many organs and on the hematologic and immunologic system. The studies were published mainly in Russian which were little known in the West because of decades of political separation, and were regarded with disapproval. Besides clinical research and application for patients, the cell biological basis was developed by the Estonian cell biologist Tiina Karu at the same time. An abstract is to be found in her work "The Science of Low-Power Laser-Therapy"