ABSTRACT
Objective: Poly [2-methacryloyloxyethyl phosphoryl choline [MPC]-co-n-buthyl methacrylate [BMA]-co-p-nitrophenyl-oxycrabonyl poly ethylene glycol-methacrylate [MEONP]] [PMBN], a biocompatible terpolymer, is a unique polymer with applications that range from drug delivery systems [DDS] to scaffolds and biomedical devices. In this research, we have prepared a monomer of p-nitrophenyl-oxycarbonyl poly [ethylene glycol] methacrylate [MEONP] to synthesize this polymer. Next, we designed and prepared a smart, water soluble, amphiphilic PMBN polymer composed of MPC, BMA, and MEONP
Materials and Methods: In this experimental study, we dissolved MPC [4 mmol, 40% mole fraction], BMA [5 mmol, 50% mole fraction], and MEONP [1 mmol, 10% mole fraction] in 20 ml of dry ethanol in two necked flasks equipped with inlet-outlet gas. The structural characteristics of the synthesized monomer and polymer were determined by Fourier transform infrared spectroscopy [FT-IR], proton nuclear magnetic resonance [H-NMR], dynamic light scattering [DLS], gel permeation chromatography [GPC], scanning electron microscope [SEM], and transmission electron microscope [TEM] analyses for the first time. We treated the polymer with two different cell lines to determine its biocompatibility
Results: FT-IR and H-NMR analyses confirmed the synthesis of the polymer. The size of polymer was approximately 40 nm with a molecular weight [MW] of 52 kDa, which would be excellent for a nano carrier. Microscopic analyses showed that the polymer was rod-shaped. This polymer had no toxicity for individual cells
Conclusion: We report here, for the first time, the full properties of the PMBN polymer. The approximately 40 nm size with an acceptable zeta potential range of -8.47, PDI of 0.1, and rod-shaped structure indicated adequate parameters of a nanopolymer for nano bio-applications. We used this polymer to design a new smart nano carrier to treat leukemia stem cells based on a target DDS as a type of bio-application
ABSTRACT
Due to the especial properties such as high growth rates, easy extraction as well as high yields, using microorganisms in comparison to other sources is more chosen for pigment production. Pigments are used in food industries as natural colorants and preservatives, they also have pharmaceutical applications. In this study, fungus Monascus purpureus PTCC 5303 have been used to produce red, orange and yellow pigments. At first significant variables were screened based on plackett-Burman design and then the optimized value of two effectivefactors such as yeast extract and K[2] HPO[4] concentrations wasoptimized byresponse surface method. Optimal levels of factors were found to be 2/75 g/L yeast extract and1/5 g/LK[2] PO[4] respectively. Antimicrobial activity of pigments was evaluated on Gram-positive foodborne bacteria under optimal conditionswhich resultsshowed inhibitory effects. Moreover Pigments production at optimal conditions in a bioreactor was evaluatedand the rate of production of red, orange and yellow pigments, 2.05, 1.55 and 0.78 [ODU/ml] were observed respectively