ABSTRACT
In this study a novel High Performance Liquid Chromatography for the assay of nystatin in oral and vaginal tablets were optimized and validated using Box-Behnken experimental design. The method was performed in the isocratic mode on a RP-18 column [30 [degree]C] using a mobile phase consisting of ammonium acetate 0.05 M buffer/ Methanol mixture [30:70] and a flow-rate of 1.0 mL/min. The specificity, linearity, precision, accuracy, LOD and LOQ of the method were validated. The method was linear over the range of 5-500 microg/mL with an acceptable correlation coefficient [r[2] = 0.9996]. The method's limit of detection [LOD] and quantification [LOQ] were 0.01 and 0.025 microg/mL respectively. The results indicate that this validated method can be used as an alternative method for assay of nystatin
Subject(s)
Chromatography, High Pressure Liquid , Pharmaceutical PreparationsABSTRACT
Water used in the pharmaceutical industry should have special physicochemical and microbiological characteristics. One of the quality items of pharmaceutical water is level of microbial content. For this reason selection of effective disinfection method is the main item for design of pharmaceutical water systems. Water systems can be maintained at temperature below 10 [degree sign]C and more than 70 [degree sign]C but maintaining of water at ambient temperature could increase of microbial content. In this condition use of disinfectant is useful for reducing of microbial content. Some of them such as chloride, ozone and hydrogen peroxide due to good results have more application. In this study, effect of three disinfectants chloride, ozone and hydrogen peroxide with 1 ppm concentration, consider on five microorganisms. Pseudomonas aeruginosa, Staphylococcus aureus, candida albicans, Aspergillus niger and E.coli. Counting and decreasing of microorganisms showed the efficiency of disinfectants. Finally ozone was selected as the best disinfectant due to high efficiency