ABSTRACT
The aerial parts of three plants belonging to Family Asteraceae: Inula crithmoides, Iphiona scarab and Jasonia montana were investigated. Phytochemicals isolated from Inula crithmoides were identified as: isopimpinellin [I], scoparone [II], psoralene [III], kaempferol-3-O-alpha-L-rhamnopyranoside [IV], kaempferol -3, 7- di-O-alpha-L-rhamnopyranoside [V], kaempferol-3-O-beta- D-glucopyranoside-7-O-alpha-L-rhamnopyranoside [VI] and isorhamnetin-3-O-beta-D-glucopyranoside-7-O-alpha-L-rhamnopyranoside [VII]. From Iphiona scarba umbelliferone [VIII], xanthotoxol [IX], marmesin [X], alpha-amyrin [XI], stigmasterol [XII] and beta-sitosterol [XIII] were isolated. Moreover, Jasonia montana was found to contain 7-hydroxy-3, 5, 6, 3',. 4'-pentamethoxy flavone [XIV], 5, 7-dihydroxy- 3, 6, 3', 4' -tetramethoxy flavone [XV] and 5, 7-dihydroxy-3, 3', 4' -trimethoxy flavone [XVI], in addition to ferulic acid, caffeic acid, chrorogenic acid, alpha-amyrin, stigmasterol and beta-sitosterol. Inula crithmoides aqueous methanolic extract showed significant inhibitory effect on mouse lymphoma [Cell line L1210] in vitro study in concentration dependant manner starting from 12.5 up to 250 microg/ml [20 - 100% inhibition], respectively. In vivo study, the results indicated that Inula crithmoides methanolic extract had significant antioxidant effect against mycotoxin [Ochratoxin A]-induced oxidative stress in rat. Iphiona scabra and Jasonia montana ethanolic extracts showed potent acute anti-inflammatory, anticoagulant and hypotenssive activities comparing to the standard drugs [indomethacine]