ABSTRACT
Statement of the Problem: Squamous cell carcinoma [SCC] is the most frequent oral cancer whose 5-year survival rate is 80% for early-detected lesions and nearly 30-50% for advanced lesions. Early detection of oral cancers and precancerous lesions can improve the patient's survival and decrease the morbidity
Purpose: This study aimed to evaluate and compare the Ki-67 and MCM3 expression in cytologic smear of oral SCC [OSCC]
Materials and Method: We examined 48 oral brush biopsies including 28 OSCC and 20 healthy non-smoking samples. Immunocytochemistry staining was performed for Ki-67 and MCM3 by using an EnVision-labeled peroxidase system, and labeling index [LI] was calculated
Results: Out of 28 OSCC cases, 27[96.4%] cases contained MCM3 positive cells and 22[78%] cases contained Ki-67 positive cells. All normal mucosa were Ki-67 and MCM3 negative. MCM3 and Ki-67 LI were significantly higher in OSCC than normal mucosa [p< 0.001]. MCM3 LI was significantly higher than Ki-67 LI in OSCC group [p< 0.001]
Conclusion: Immunocytologic evaluation of Ki-67 and MCM3 can be used for early detection of OSCC. Furthermore, MCM3 may be a more sensitive cytologic bi-omarker than Ki-67 in SCC patients
Subject(s)
Humans , Carcinoma, Squamous Cell/genetics , Ki-67 Antigen , Minichromosome Maintenance Complex Component 3 , Precancerous Conditions , Gene Expression , ImmunohistochemistryABSTRACT
The aim of this study was to ascertain the prevalence of human papillomavirus [HPV] 16/18 in oral squamous cell carcinoma [OSCC] and normal oral mucosa in Iranian individuals with OSCC and their association with various risk factors. The investigation followed a hospital-based case-control design. Cases were individuals with diagnosed OSCC, age- and sex-matched with controls. Sixty-six paraffin embedded tissue samples [39 OSCC and 27normal oral mucosa] were studied. DNA was purified to amplify HPV-DNA using HPV type specific primers-mediated polymerase chain reaction [PCR] method. Mean age was 64.2 +/-14 years in cases [range 25-81 years], and 63.5 +/-13.5 years in controls [range 22-78 years]. Forty-three percent [17/39] of OSCC but none of normal oral mucosa were positive for HPV 16/18 DNA [p = 0.001]. The most frequent type in patient group was HPV-16 type. The prevalence rate was high older in age [>50 years] and gender [male] and tongue tissue but no significant association was found [p=0.7, p=0.5 and p=0.42 respectively]. We found a significant association of HPV16/18 with oral squamous cell carcinoma. Also, HPV16/18 is a co-factor in oral carcinogenesis. In addition, also it is suggested that HPV is as a risk factor independent of alcohol and tobacco can be effective in creating OSCC
ABSTRACT
Statement of the Problem: The expression of minichromosome maintenance-3 [MCM3] proteins and their diagnostic value in oral mucosal dysplasia and squamous cell carcinoma [SCC] is not well known.
Purpose: This study was conducted to evaluate the usefulness of minichromosome maintenance 3 [MCM3] as a biomarker for diagnosis of oral premalignant lesions and SCC.
Materials and Method: In this study, 31 oral SCC, 20 dysplastic epithelium and 20 controls were selected and immunohistochemical staining was done for MCM3. ANOVA, Tukey HSD, Mann-Whitney and Kruskal-Wallis tests were used to compare the groups and the correlation between different grades
Results: There was increasing trend of MCM3 from control to dysplastic epithelium and from dysplastic epithelium to SCC both in suprabasal layers and in total epithelial layers. MCM3 expression was elevated with increasing the grade of dysplasia, but there was no statistically significant difference [p= 0.93]. The expression was also increased in high grades of SCC compared to lower grades
Conclusion: MCM3 can be used as a useful biomarker in the diagnosis of premalignant lesions and oral SCC
ABSTRACT
Statement of the Problem: Matrix metalloproteinases [MMPs] have been implicated in the pathogenesis of certain diseases and cancers via tissue destruction and can be secreted into the blood stream. MMP9 expression in the salivary gland tissue was evaluated but their serum level in the salivary gland tumors was not studied
Purpose: The aim of our study was to determine the concentration of serum MMP-9 in healthy participants and in patients with salivary gland tumor
Materials and Method: Using an ELISA kit, the circulating levels of MMP-9 in sera from 58 patients with salivary gland tumor [31 pleomorphic adenoma, 17 adenoid cystic carcinoma and 10 mucoepidermoid carcinoma] and 30 healthy controls was assessed
Results: The serum MMP9 level in patients with salivary gland tumors [380.0 +/- 301.3 pg/ml] also patients with benign tumors [354.3 +/- 218.7 pg/ml] [354.3 +/- 218.7 pg/ml] were significantly lower than that in the healthy group [727.4 +/- 624.6 pg/ml] [Respectively p= 0.02 and p= 0.01]. Mean serum MMP9 concentration in malignant tumors was [402.3 +/- 441.8pg/ml] higher than benign tumors [354.3 +/- 218.7 pg/ml] but the difference was not significant [p= 0.9]
Conclusion: Our results showed that serum level of MMP9 decreased in patients with salivary gland tumors which suggest that MMP9 may not have a potential role in development and pathogenesis of salivary gland tumor
ABSTRACT
Oral lichen planus is a chronic inflammatory disease with a poorly understood etiology. The role of angiogenesis in the development of different chronic inflammatory diseases is of great concern. Vascular endothelial growth factor [VEGF] is an important regulator of angiogenesis. We aimed to evaluate the serum level of VEGF in patients with oral lichen planus compared with normal individuals and consider its clinical significance. In this case-control study, 36 serum samples from patients diagnosed with oral lichen planus admitted to the Oral Medicine Department of the School of Dentistry at Shiraz University of Medical Sciences [14 men, 22 women, mean [ +/- SD] age: 38.8 [ +/- 6.07] years] and 23 serum samples from healthy individuals [9 men, 14 women, mean [ +/- SD] age: 38.7 [ +/- 4.9] years] were collected. VEGF concentration was measured using the ELISA method. The Mann-Whitney test was used for statistical analysis. The serum VEGF level was significantly higher in patients with oral lichen planus compared with the healthy controls [112.97 [ +/- 63.2] vs. 66.21 [ +/- 56.2] ngr/ml, P < 0.001]. A similar difference was also observed between the two types of oral lichen planus, being more pronounced in the erosive form [P < 0.001]. Serum VEGF can be used as a useful and suitable marker to scrutinize the disease activity
ABSTRACT
Galectin-3, a member of the family of beta-galactoside-binding animal lectins, has been implicated in tumor invasion and metastasis. Using an ELISA kit, we assessed the circulating levels of galectin-3 in sera from 40 oral squamous cell carcinoma [OSCC] patients and from 43 healthy controls. Serum galectin-3 levels in OSCC patients were significantly higher [5.1 +/- 2.5 ng/ml] when compared with healthy controls [2.6 +/- 3.07 ng/ml, P<0.0005]. There was no apparent correlation between serum galectin-3 concentration and clinico-pathological features such as stage, tumor size, nodal status, distant metastasis and histological grade. This result suggests that, in addition to other tests, measurement of serum galectin-3 concentrations can be utilized as an adjuvant test for establishing a diagnosis of OSCC