ABSTRACT
A serious health threat affecting the T2DM group is evident more cases T2DM are diagnosed. In this research, we choose to research into all of this possible mechanism of 3T3-L1 Cell lines and Molecular Docking studies Schrodinger software identified Vitamin D, Omega-3, and 6 PUFAs (EPA DHA & AA) Compounds of hydrophilic and hydrophobic pocket throughout molecular modeling besides T2DM. A group of three analog VDRs is being developed for discovery treatment with T2DM. Its use as it was agreed to run a molecular cell culture and docking study. Recognize the binding method involving the compound in T2DM through ADME prediction. The molecular dynamics simulation was enhanced by confirmation of the strength of the possible composite binding. Based on the computational results, the Omega-3 and 6 PUFAs compound encourages energy interaction. The composite contains an in vitro anti-diabetic activity; the compounds have clearly shown that they are active on T2DM. Our studies provide vital information on the findings of the bimolecular T2DM inhibitors.
ABSTRACT
The solubility of calcium phosphate crystallization in vitroby an deproteinized concoction of Basella rubraplant was investigated by weight reduction assay. Soluble oxalate of the plant arepartially removed prior to extraction of plant material. The weight variation and dissolution results reveals that the concoction showed considerable increased activity at the highest concentration used. The results indicate that the calcium phosphate crystal inhibition activity exhibited by the plant material may be due to their calcium phosphate solubilising capacity of phytoconstituents present in them. Regular consumption of an concoction of our plant would be helpful in calculi prophylaxis
ABSTRACT
The methanol extracts of Mahonia leschenaultii Takeda [Berberidaceae] root and root bark were tested for antibacterial potential against Escherichia coli [NCIM 2068], Pseudomonas aeruginosa [NCIM 2053], Staphylococcus aureus [NCIM 2492] and Staphylococcus epidermidis [NCIM 2493], on nutrient agar medium and nutrient broth using ampicillin trihydrate as standard drug. For antifungal study strains used were Trichophyton lignorum [NCIM 1195] and Candida crusei [NCIM 3129], on Sabourauds dextrose agar [SDA] and Sabourauds dextrose broth [SDB] by cup plate method using amphotericin B as standard drug. The results showed that all extracts exhibited significant activity against all the selected strains of bacteria and relatively more against Staphylococcus epidermidis. The antifungal activity was less significant when compared with antibacterial activity
Subject(s)
Anti-Infective Agents , Plant Structures , Plant Roots/chemistry , Plant Bark/chemistry , PhytotherapyABSTRACT
The quantitative ash values and extractive values were determined for the roots of Bryonopsis laciniosa Naud [family - Cucurbitaceae]. The roots were extracted successively with various organic solvents and the subsequent fluorescence analysis was carried out. The extracts were subjected to qualitative phytochemical analysis and presence of steroids and saponins was found. One steroidal compound was isolated from the ethanolic extract by column chromatography. The gross behavioural and toxicity studies of the ethanolic extract revealed marked analgesia at the tested dose levels of 1000, 2000 and 3000 mg/kg body weight, but no mortality was observed at the end of 72 hours. The ethanolic extract was screened for anti-inflammatory and analgesic activities in two different dose levels of 200 and 400 mg/kg by carrageenan-induced paw oedema method in rats and writhing test in mice respectively. The extract showed significant anti-inflammatory and analgesic activities at both the dose levels tested. However, the degree of activity observed with the extract in the dose of 400 mg/kg revealed a higher value, which confirms the dose dependent nature of the extract. Present findings suggest that the roots of Bryonopsis laciniosa Naud are endowed with potential anti-inflammatory and analgesic activities
Subject(s)
Animals, Laboratory , Plant Roots , Plants, Medicinal , Fluorescence , Saponins , Plant Extracts , Chromatography , Rats , Mice , Carrageenan , Anti-Inflammatory Agents , Analgesics , Random AllocationABSTRACT
The stem bark, stem wood, root bark and root wood of Mahonia leschenaultii Takeda [Family -Berberidaceae] were extracted successively with various organic solvents and the subsequent fluorescence analysis was carried out. The quantitative ash values and extractive values were determined. Berberine was isolated and characterized. The quantitative estimation of berberine in the various extracts was carried out by High performance thin layer chromatography method. The antibacterial and antifungal activity of methanolic extracts were carried out by agar diffusion and serial dilution method. The methanolic extracts showed prominent activity against Staphylococcus aureus in comparison to other strains
ABSTRACT
The stem bark, stem wood, root bark and root wood of Berbetis tinctoria Lesch. were extracted successively with various organic solvents and the subsequent fluorescence analysis was carried out. The quantitative ash values and extractive values were determined. Berberine was isolated and characterized. The quantitative estimation of berberine in the various extracts was carried out by HPTLC technique. The antibacterial and antifungal activities of methanolic extracts were carried out by cylinder-plate and serial dilution method. The methanolic extracts showed prominent activity against Staphylococcus aureus in comparison to control [Ampicillin trihydrate]