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@#Objective To develop and verify a double antibody sandwich ELISA method for quantitative detection of TrypLE.Methods The optimal concentration of capture antibody and detection antibody were determined by orthogonal experiments to develop TrypLE double antibody sandwich ELISA quantitative detection method,which was verified for linear range,specificity,limit of detection(LOD),limit of quantitation(LOQ),accuracy and reproducibility.A variety of biological products were detected by the developed method to verify the applicability.Results The TrypLE double antibody sandwich ELISA quantitative detection method was established by using 3 μg/mL capture antibody and 15 000 times dilution of detection antibody,with a linear range of 0.41 ～ 40.00 ng/mL,a LOD of 0.258 ng/mL,a LOQ of 0.5 ng/mL.The measurement deviation was less than 5% and the CV of reproducibility verification was less than 5% when detecting standards and samples.The recovery rates of different types of samples were within 80% ～ 120%.Conclusion The established TrypLE double antibody sandwich ELISA quantitative detection method accurately,effectively and quickly detected residual amount of TrypLE in various types of biological products with good specificity,accuracy and reproducibility.
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OBJECTIVES@#To investigate the diversity of peripheral blood T cell receptor (TCR) β chain complementarity-determining region 3 (CDR3) based on immune repertoire sequencing in neonates with sepsis and the possible pathogenesis of neonatal sepsis.@*METHODS@#A total of 12 neonates with sepsis were enrolled as the case group, and 9 healthy full-term infants, matched for gestational age, birth weight, and age, were enrolled as the control group. Omega nucleic acid purification kit (SQ blood DNA Kit II) was used to extract DNA from peripheral blood samples, TCR β chain CDR3 was amplified by multiplex PCR, and then high-throughput sequencing was performed for the products to analyze the diversity of TCR β chain CDR3 and the difference in expression.@*RESULTS@#The length and type of TCR β chain CDR3 were similar between the case and control groups, and Gaussian distribution was observed in both groups. With D50 and Shannon-Wiener index as the evaluation indices for diversity, the case group had a significantly lower diversity of TCR β chain CDR3 than the control group (@*CONCLUSIONS@#There is a significant change in the diversity of TCR β chain CDR3 in the peripheral blood of neonates with sepsis, suggesting that it might be associated with the immune pathogenesis of neonatal sepsis.
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Humans , Complementarity Determining Regions/genetics , High-Throughput Nucleotide Sequencing , Multiplex Polymerase Chain Reaction , Neonatal Sepsis , Receptors, Antigen, T-Cell, alpha-beta/geneticsABSTRACT
Objective@#To investigate the factors associated with the patient's adherence to screening in the five years before the diagnosis of hepatocellular carcinoma (HCC) related to chronic hepatitis B ( CHB ), so as to provide reference for improving the screening rate.@*Methods@#From June 2016 to April 2018, the patients with newly diagnosed HCC and a history of CHB for more than five years in Southwest Hospital in Chongqing were interviewed. The information about socio-demographic characteristics, health status, medical care and HCC screening in the past five years were collected. A multivariate logistic regression model was used to analyze the factors associated with adherence to screening. @*Results@#Among 420 participants, 140 ( 33.33% ) adhered to HCC screening, 124 ( 29.53% ) had irregular/incomplete screening, while 156 ( 37.14% ) never had screening. The proportion of early-stage HCC at diagnosis was significantly higher in patients who adhered to screening ( 77.14% ) than that in patients who had irregular/incomplete screening (35.48%) or no screening ( 12.82% ) and the differences were statistically significant ( P<0.05 ). The multivariate analysis demonstrated that five factors were significantly associated with patient's adherence to screening, including education level of high school and above ( OR=2.346, 95%CI: 1.370-4.017), family history of HCC ( OR=2.795, 95%CI: 1.457-5.362 ), history of chronic diseases ( OR=3.860, 95%CI: 2.052-7.262), acceptance of antiviral therapy ( OR=17.816, 95%CI: 9.702-32.716 ) and specialized clinic visits ( OR=8.332, 95%CI: 1.588-43.710 ).@*Conclusions@#Adherence to screening is conducive to the early detection of HCC, but the screening rate is low in the patients with CHB. Education level, history of HCC, health status and medical status are significantly related to screening adherence.
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Purpose@#This study sought to understand Taiwanese women’s decisional experiences regarding prenatal screening procedures and diagnostics. @*Methods@#A hermeneutic phenomenological design guided semistructured interviews with 33 women who were 36 weeks pregnant. Data were collected between February and October 2016. Verbatim transcripts were analyzed following hermeneutic circle to cocreate an understanding of Taiwanese women’s decision-making in prenatal screening and diagnostics. @*Results@#Women’s existential experiences were derived from their decision-making process on prenatal screening procedures and diagnostics for chromosomal aneuploidy. These decisional experiences were captured by four theme clusters and eight themes, which were inductively derived from 16 meaning units: (1) accessing health information; (2) considering what was best for my baby; (3) considering family finance; and (4) feeling anxiety posttest. @*Conclusion@#Participants made informed choices on several prenatal screening procedures, ostensibly, based on their personal values and considerations. During the decision-making process, often-cited benefits of genetic screenings were emphasized, but test limitations were often unheeded. A fundamental need for supportive information in decision making was further identified with recommended strategies. Hence, a revision of traditional genetic counseling approaches is recommended. As genomics technologies are increasingly available during antenatal services, women should be sufficiently educated about them to support decision making.
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Purpose@#This study sought to understand Taiwanese women’s decisional experiences regarding prenatal screening procedures and diagnostics. @*Methods@#A hermeneutic phenomenological design guided semistructured interviews with 33 women who were 36 weeks pregnant. Data were collected between February and October 2016. Verbatim transcripts were analyzed following hermeneutic circle to cocreate an understanding of Taiwanese women’s decision-making in prenatal screening and diagnostics. @*Results@#Women’s existential experiences were derived from their decision-making process on prenatal screening procedures and diagnostics for chromosomal aneuploidy. These decisional experiences were captured by four theme clusters and eight themes, which were inductively derived from 16 meaning units: (1) accessing health information; (2) considering what was best for my baby; (3) considering family finance; and (4) feeling anxiety posttest. @*Conclusion@#Participants made informed choices on several prenatal screening procedures, ostensibly, based on their personal values and considerations. During the decision-making process, often-cited benefits of genetic screenings were emphasized, but test limitations were often unheeded. A fundamental need for supportive information in decision making was further identified with recommended strategies. Hence, a revision of traditional genetic counseling approaches is recommended. As genomics technologies are increasingly available during antenatal services, women should be sufficiently educated about them to support decision making.
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· AIM:To compare the clinical curative effect of triamcinolone acetonide and Ranibizumab on diffuse diabetic macular edema (DME).· METHODS:We collected 84 cases of patients with diffuse DME treated in our hospital from February 2016 to May 2017.According to 1:1 matching method,they were divided into Ⅰ,Ⅱ groups.They were all treated with laser photocoagulation.Preoperative auxiliary application of triamcinolone acetonide was given to Group Ⅰ,while Group Ⅱ received preoperative application of ranibizumab.After treatment,the efficacy of the two groups were analyzed and compared.· RESULTS:The total clinical efficiency of Group Ⅱ at 3mo after treatment was 93％,higher than that of Group Ⅰ (77％;x2 =4.981,P =0.025).Compared with before treatment,BCVA and CMT of the two groups at each time after treatment were significantly improved (P＜ 0.05).BCVA of Group Ⅱ at 1 and 3mo after treatment was better than that of Group Ⅰ (P＜0.05);CMT of Group Ⅱ at 1,3 and 6mo after treatment improved more than that of Group Ⅰ,with significant difference (P ＜ 0.05);occurrence rate of adverse reactions Group Ⅰ and Group Ⅱ were 17％ and 13％ with no significant statistical difference (X2 =0.243,P=0.621).There were no serious adverse reactions such as retinal detachment,endophthalmitis or cataract in the two groups.· CONCLUSION:Compared with triamcinolone acetonide,the effect of ranibizumab on diffuse diabetic macular edema is better,and has high clinical value.
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OBJECTIVE: To investigate and analyze the use of TCM national essential medicines in our hospital, so as to provide reference for clinical rational use. METHODS: The variety number, consumption sum and constituent ratio, defined daily dose system(DDDs), defined daily cost(DDC)and drug use distribution in departments of TCM national essential medicines in our hospital were statistic analyzed during 2013-2015. RESULTS: The variety number of TCM national essential medicines were relatively stable in our hospital, but the consumption sum and proportion showed a downward trend year by year. The consumption sum and DDDs of essential medicines changed less during 3 years, and the drug with large sales amount were relatively fixed, which showed that the clinical selection of essential medicines were relatively centralized, and the category and DDDs continuity of essential medicines was fairly good. The concentration ratio of TCM essential drug use were lower in clinical departments, and the consumption sum ratio were lower in TCM department. CONCLUSION: We must pay more attention to the rational use of essential medicines, and formulate relevant policies and measures to encourage clinical use essential medicines preferentially.
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<p><b>OBJECTIVE</b>To investigate the skill and evaluate the accuracy for application of guide combined with probing the internal wall of pedicle screw trajectory for subaxial cervical pedicle screw placement.</p><p><b>METHODS</b>Subaxial cervical pedicle screw was inserted in 11 patients by the guide combined with probing the internal wall of pedicle screw trajectory from January 2014 to October 2016, including 7 males and 4 females with an average age of 48.1 years(ranged 32 to 63 years). There were 4 cases with cervical spondylotic myelopathy, 4 with fracture and dislocation of cervical vertebrae, 1 with cervical cord injury without fracture and dislocation, and 2 with atlantoaxialfracture and dislocation. The target pedicle's diameter, optimal entry point, sagittal angle and cross-sectional angle were measured by CT before operation. During operation, the pedicle screw inserted angle was controlled by a guide with a self-designed protractor and probed the internal wall of pedicle screw trajectory as medial safety margin of insertion screw. The accuracy of cervical pedicle screw was evaluated by CT with classification of four grades and assessed whether there was injury of spine cord or vertebral artery postoperatively.</p><p><b>RESULTS</b>Seventy-one cervical pedicle screws were placed among 11 patients, and no one had been found with clinical manifestations of injury of spine cord (or nerve root) or vertebral artery after operation. According to postoperative CT scan for evaluating the grade of screw position, 52 screws were in grade 0, 13 in grade 1, 4 in grade 2, 2 in grade 3, and 91% (65/71) located in good position. In total, 6 screws were incorreted in placement, and 4 cases of them broke medial wall and 2 cases broke lateral wall.</p><p><b>CONCLUSIONS</b>The method of probing the internal wall of pedicle screw trajectory for subaxial cervical pedicle screw placement is safe and reliable, but the studying curve is long. Probing the internal wall of pedicle screw trajectory and controlling the insertion angle by guide with a protractor are key points of this technology.</p>
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OBJECTIVE: To analyze the features of high-risk drugs prescriptions for elderly outpatients in our hospital, so as to provide references for establishing the prescription comment method of high-risk drugs used by elderly outpatients and promote the rational drug use. METHODS: The number and amount of money of high-risk drugs prescriptions for elderly outpatients were counted, and the amount of high-risk drugs were sorted, so as to find out the features of high-risk drugs use in elderly outpatients and determine the prescription comment catalogue. According to "The hospital prescription comment management specification (Trial)", and combined with the use of high-risk drugs and physical characteristics of elderly patients, the prescription comment technical manual of high-risk drugs used by elderly outpatients was established, and the key drugs were commented for demonstration purpose. RESULTS: Elderly patientsoccupied a high proportion in our hospital, and the amount of money of single prescription was also high, which indicated that the elderly patients had more complex drug use. Through analysis of high-risk drugs prescriptions, the comment catalogue was established. And through empirical review of the prescriptions of key drugs, it was found that the proportion of unreasonable prescriptions was high, with variousproblems among different prescriptions. CONCLUSION: This study established the prescription comment technical manual of high-risk drugs used by elderly outpatients, which should become an important measure to prevent and reduce drug-induced diseases in elderly patients, and improve the effectiveness and safety of clinical medication.
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<p><b>OBJECTIVE</b>To investigate clinical effects of strategy by stages for preventing respiratory complications of patients with acute cervical spinal cord injury (ACSCI).</p><p><b>METHODS</b>From September 2009 to May 2013,the clinical data of 91 patients with ACSCI underwent surgery were retrospectively analyzed. Among the patients, 42 patients were divided into in-stages group, including 30 males and 12 females with an average age of 50 years old (ranged 28 to 76) which were treated with strategy by stages for preventing respiratory complications; others 49 patients which were not treated with the strategy regarded as control group, including 38 males and 11 females with an average age of 47 years old (ranged 30 to 77). All of them had definite history of trauma, and were admitted to orthopaedics within 48 h after trauma. In in-stages group, respiratory muscle strength training, high-dose ambroxol using and other treatment were performed to prevent respiratory complilcations according to preoperative, intraoperative and postoperative stage. While in control group, there were no systematic and effective measures utilized. Chi-square test was used to evaluate the difference for respiratory complications rate, the rate of tracheostomy or intubation and mortality caused by the respiratory complications between two groups.</p><p><b>RESULTS</b>Ten patients developed with respiratory complications in in-stages group (7 patients with pneumonia, 1 with atelectasis and 2 with respiratory failure), among which 3 patients underwent tracheostomy or intubation. In control group, 24 patients developed with respiratory complilcations (15 with pneumonia,3 with atelectasis and 6 with respiratory failure), among which 11 patients underwent tracheostomy or intubation. There was significant difference between two groups (χ2 = 6.12, 4.07; P = 0.013, 0.044). Five patients died because of respiratory complications, one case were in in-stages group and 4 in control group. There was significant difference between two groups (χ2 = l.39, P = 0.238).</p><p><b>CONCLUSION</b>The strategy by stages is an effective method for preventing respiratory complications of ACSCI and can reduce the respiratory complications rate and improve the prognosis of respiratory complications.</p>
Subject(s)
Adult , Aged , Female , Humans , Male , Middle Aged , Acute Disease , Cervical Cord , Wounds and Injuries , Pneumonia , Pulmonary Atelectasis , Respiratory Insufficiency , Retrospective Studies , Spinal Cord Injuries , TracheostomyABSTRACT
OBJECTIVE: To analyze the clinical use Chinese herbal pieces in Beijing hospital, so as to provide references for the clinical rational medication. METHODS: The clinical dose, medication frequency, herb number, herb efficacy distribution and poisonous herb usage the traditional Chinese medicine (TCM) prescriptions in Beijing hospital were investigated, andwere contrasted with those described in Pharmacopoeia, classicalprescription and proved prescription, so as to deeply study the laws dose-effect-poisonous-ness relationship for TCM. RESULTS: There were about 500 Chinese herbal pieces, 28 poisonous herbs and 50 000 prescriptions per year in our hospital. Statistics showed that the doses some Chinese herbal pieces included poisonous herbal pieces were beyond the Pharmacopoeia regulated doses; herb numbers in some prescriptions were much larger than classicalprescription and proved prescription; tonic and antipyretic medicines were mostly used in these prescriptions. CONCLUSION: The clinical use TCM decoction pieces in our hospital were basically rational. The efficacy distribution the Chineseherbal pieces were consist with the gerontology characteristic and disease spectrum our hospital. However, there were some problems, such as large herb numbers and overdose some Chinese herbal pieces included poisonous herbal piecesin these prescriptions, should be further researched and improved.
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OBJECTIVE: To investigate the blocking effect of bergapten on cell cycle in nasopharyngeal carcinoma (NPC) cells. METHODS: The inhibition was tested by CCK-8 assay after the NPC cells (CNE-2 and HONE-1) being exposed to bergapten for 48 h. The bergapten concentrations were as follows: 1, 10 and 100 μmol·L-1. The cell cycle was assayed by PI staining and following flow cytometry analysis, and the CDK4, CDK6, CDK2, Cyclin D1, Cyclin E2, β-catenin, GSK-30 and p-GSK-3p (S9) protein levels were detected by Western blotting. RESULTS: Bergapten was able to significantly inhibit CNE-2 and HONE-1 NPC cancer cell proliferations, and the effect was dose-(CNE-2: r=0.926, P<0.05; HONE-1: r=0.959, P<0.05) and time-dependent (CNE-2: r=0.991, P<0.05; HONE-1: r=0.963, P<0.05). Drug treatment induced a block in the G0/G1 phase and decreased protein levels of CDK4, CDK6, CDK2, Cyclin D1, Cyclin E2 and β-catenin, but the protein levels of GSK-3β and the phosphorylation of p-GSK-3β (S9) didn't change. CONCLUSION: Bergapten can block cells cycle and inhibit NPC cells' proliferations, and those effects might be related to suppressing of cells cycle-related protein and interfering with the wnt/p-catenin signal pathway.
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AlM: To explore the different ages of congenital nasolacrimal duct obstruction in infants, take different treatment methods at different times. METHODS:The 87 cases of 102 children were divided into three different age groups: the first group of 25d-3mo of age 21 cases 26 eyes; The second group >3mo-7mo 31 cases 36 eyes;The third group >7-24mo of age 35 cases 40 eyes. For the first group of infants, the implementation of the lacrimal sac nasolacrimal duct massage + eye drops; for the second group of infants, carry lacrimal pressure washing treatment; for the third group of infants, the implementation of the nasolacrimal duct probing treatment. RESULTS: The first group of children through the nasolacrimal duct sac massage + drops tobramycin eye drops treatment unobstructed 12, the cure rate was 46. 2%;The second group of children through pressurized irrigation treatment lacrimal patency by 33, the cure rate was 91. 7%; The third group of children through the nasolacrimal duct probing unobstructed 36 treatment, the cure rate was 90. 0%. The second and third group were better than the first group (χ2=15. 71, P0. 05). CONCLUSlON:lnfants with congenital nasolacrimal duct obstruction should distinguish between ages, taking different treatments, in order to obtain a better therapeutic effect, and lacrimal pressure washing is the preferred way of treating infants with congenital nasolacrimal duct obstruction.
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OBJECTIVE: To analyze the varieties, pharmaceutical utilization, adverse drug reaction(ADR) and majority problems in clinical use of poisonous traditional Chinese medicines in Beijing hospital, so as to provide references for their clinical rational use. METHODS: The varieties of Chinese patent medicines contain poisonous ingredients in Beijing hospital were surveyed, and their poisonous ingredients, drug categories, consumption sums, defined daily dose system(DDDs), adverse reactions and rational uses were analyzed. RESULTS: There were more than 300 Chinese patent medicines in Beijing hospital, in which 105 contained poisonous ingredients. Pharmaceutical utilization research indicated that most of the poisonous Chinese patent medicines with much higher consumption sums and DDDs were used for orthopedics and cardio-cerebrovascular diseases. In the statistics of ADR for 5 years in our hospital, 185 ADR reports of Chinese patent medicines were collected, among which poisonous Chinese patent medicines were involved in 49 ADR reports. CONCLUSION: The poisonous traditional Chinese medicines play important roles in clinical treatment, however, the adverse reaction rates of these medicines were much higher and may cause serious outcome. Therefore, their poisonous constituents and action mechanisms should be researched in depth constantly and the research should be linked with their clinical practices, so as to ensure the safe and rational use of poisonous traditional Chinese medicines.
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Although guidelines and formulas have been developed through clinical practice to define infusion rate and volume, over- and under-resuscitation are still common, followed by increasing morbidity and mortality. In order to establish an effective management for early fluid resuscitation, the clinical decision support system (CDSS) has been established. The CDSS, by utilizing information systems coupled with decision support technology, could provide recommendations for the amount of fluid to be infused based on measured biological response. The results showed that patients treated with CDSS had a significantly lower mortality, increased ventilator-free days, and ICU-free days as compared with those treated with traditional fluid management. This article reviews the concepts as well as the result of recent clinical studies of CDSS for burn patients.
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Humans , Burns , Therapeutics , Decision Support Systems, Clinical , Fluid TherapyABSTRACT
<p><b>OBJECTIVE</b>To investigate clinical features and treatments of cervical spinal injury in aged patients.</p><p><b>METHODS</b>From July 2006 to October 2011, 27 patients with cervical spinal cord injury were divided into experiment group, including 20 males and 7 females with an average age of 69 years old (ranged 65 to 78 years old). The other 22 patients with the same degree of cervical spianl cord injury and the same period hospitalization were chosen to regard as control group, including 16 males and 6 females with an average age of 47 years old (ranged 38 to 65 years old). All of them had clear history of trauma, and were admitted to hospital within one week. Clinical data and result of follow up were evaluated between two group. The function of the spinal cord was measured by Frankel classification at admission and final follow-up.</p><p><b>RESULTS</b>All patients were followed up for 1 year except 6 died in aged group, among which 3 paients with complications were died during hospitalization and others were out of hospital. Twenty-three cases had accompanied diseases and 24 cases occurred complications in aged group. Contrast to control group, this difference was statistically significant. At the final follow-up, there were 2 cases at Frankel grade E in aged group and 6 cases in control group.</p><p><b>CONCLUSION</b>In comparison with control group, there are more accompany diseases and higher complications and mortality rates in aged group, while poor improvement of spinal cord function. This patients were mainly treated with operation, but operation method should simplify and mainly focus on effective decompression and reconstuction stability and avoid expansion operation. It's nesscerry to pay more attention to prevent and treatment of respiratory complication.</p>
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Adult , Aged , Female , Humans , Male , Middle Aged , Cervical Vertebrae , Wounds and Injuries , Postoperative Complications , Epidemiology , Mortality , Therapeutics , Spinal Injuries , General SurgeryABSTRACT
<p><b>OBJECTIVE</b>To establish an in vitro pig iliac artery endothelial cells (PIECs) senescence model using carbamide peroxide (CP).</p><p><b>METHODS</b>MTT assay and DAPI staining were used to define the optimal concentration of CP for inducing to the PIECs senescence model. Cellular morphology, MTT assay, EdU labeling, SA-β-gal staining and cell scratch test were performed to analyze the cell growth kinetic, proliferative activity, aging ratio and migratory activity difference post CP induction. PI signal staining flow cytometry was used to analyze the cell cycle distribution difference of cells before and after CP induction.</p><p><b>RESULTS</b>The optimal CP concentration was 40 µmol/L to induce PIECs senescence. After 1 h treatment with 40 µmol/L CP, the PIECs presented typical aging form with lager and more rounded shapes. Compared with control group, the proliferative activity and the migratory distance of CP group were significantly decreased; the SA-β-gal staining positive ratio was significantly increased; the data of mitotic cycle distribution with flow cytometry analysis showed that most cells were arrested at G(1)/G(0) phase.</p><p><b>CONCLUSION</b>CP could efficiently induce pig iliac artery endothelial cell senescence in vitro.</p>
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Animals , Cells, Cultured , Cellular Senescence , Endothelial Cells , Cell Biology , Endothelium, Vascular , Cell Biology , Models, Cardiovascular , Peroxides , Pharmacology , Swine , Urea , PharmacologyABSTRACT
<p><b>OBJECTIVE</b>To evaluate clinical effects of pedicle fixation without bone fusion in treating thoracolumbar fractures through paraspinal approach.</p><p><b>METHODS</b>From January 2006 to January 2009, 25 patients (15 males and 10 females) with thoracolumbar fractures were treated. The average age was 39.3 years,ranged from 17 to 49 years. According to classification, flexion fracture in 7 cases, brust fracture in 18 cases. There were no nervous injury, and radiology information showed the angle of sagittal vertebral body >20 degrees or collapse of vertebral body >40%,without vertebral injury. The operation were performed at 3 to 7 days after injury (mean 5 day). Internal fixation implants were removed at 8 to 12 months after operation. The height, kyphosis angle were measured before operation, 1 week and 24 months after operation,and Oswestry disability index (ODI) were compared before and after operation.</p><p><b>RESULTS</b>All patients were followed up for 24 months. Among them, 1 case was followed up at 30 months after operation. The operation time ranged from 70 to 110 (mean 90) minutes, the blood loss was 120 to 280 (mean 200) ml. The height of vertebral body and kyphosis angle were obviously corrected, and had significant differences between postoperation immediately and at the final follow-up (P<0.05). There were no differences after remove of internal fixation (P>0.05). The final ODI was (5.36 +/- 1.21)%, had statistical differences compared with preoperation (P<0.05).</p><p><b>CONCLUSION</b>For flexion and burst thoracolumbar fractures without nervous injury, pedicle fixation without bone fusion is a good method,which has advantages of minimally invasive, rapid recovery, and maintain spinal motion segment.</p>
Subject(s)
Adolescent , Adult , Female , Humans , Male , Middle Aged , Young Adult , Fracture Fixation, Internal , Methods , Lumbar Vertebrae , Diagnostic Imaging , Wounds and Injuries , General Surgery , Spinal Fractures , Diagnostic Imaging , General Surgery , Spinal Fusion , Methods , Thoracic Vertebrae , Diagnostic Imaging , Wounds and Injuries , General Surgery , Tomography, X-Ray Computed , Treatment OutcomeABSTRACT
<p><b>OBJECTIVE</b>To obtain allelic loss mapping and define the minimal lost region on chromosome 1q21 in gastric carcinomas, and explore role of 1q21 loss of heterozygosity (LOH) in the development and progression of gastric carcinogenesis.</p><p><b>METHODS</b>Using 7 high-density microsatellite markers and PCR method, lq21 LOH was analyzed in 30 paired specimens of fresh gastric carcinoma, and the relation between 1q21 LOH and the clinicopathological features of the malignancy was tested.</p><p><b>RESULTS</b>The LOH frequency on chromosome 1q21 from these gastric carcinoma tissues reached 60% (18/30). The LOH frequencies of the microsatellite markers D1S514, D1S2696, D1S498, D1S305, D1S2624, D1S2635 and D1S2702 were 13.3%, 10%, 20%, 23.3%, 33.3%, 40% and 23.3%, respectively. The minimal lost region on 1q21 LOH in the gastric carcinoma tissues was located in the region between D1S2624 and D1S2707, in the vicinity of D1S2635. No significant relations of 1q21 LOH to the patients' age, gender, location of the primary foci, clinical staging, or the tumor differentiation were noted (P>0.05), but 1q21 LOH was correlated to lymph node metastases of the malignancy (P<0.05).</p><p><b>CONCLUSION</b>Higher frequency of 1q21 LOH occurs in gastric carcinoma cells, suggesting the presence of potential tumor suppressor genes closely associated with gastric carcinogenesis near the region of D1S2635.</p>
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Female , Humans , Male , Middle Aged , Adenocarcinoma , Genetics , Chromosome Mapping , Chromosomes, Human, Pair 1 , Genetics , Loss of Heterozygosity , Lymphatic Metastasis , Microsatellite Repeats , Stomach Neoplasms , GeneticsABSTRACT
<p><b>OBJECTIVE</b>To investigate the stress intensity and distribution in the dentine of intact endodontically treated mandibular second premolar under three different restorations.</p><p><b>METHODS</b>Four three-dimensional finite element models were constructed by the data of spiral CT: 1) Mandibular second premolar restored directly by full crown: 2) Fill the perforating cavity with resin material and full crown restoration; 3) Cast nickel chromium (NiCr) alloy post core plus full crown restoration; 4) Fiber post and resin core plus full crown restoration. Load was applied on the buccal inclined surface near the top of the buccal cusp and the intensity and distribution of the maximum tensile stress and von Mises stress in the dentine were compared.</p><p><b>RESULTS</b>When the tooth was restored with NiCr alloy post core, an obvious stress concentration area was observed in the dentine next to the top of the post. When the fiber post was used, there was little stress concentration area around the top of the post, and the stress distribution in the dentine was almost the same as the control group. No difference of the intensity and distribution of the stress was found with the control group when no post was used and the perforating cavity was just filled with resin material.</p><p><b>CONCLUSION</b>Compared with cast metal post, fiber post is more suitable for post-core crown restorations. In cases with sufficient coronal tooth substance, post is not absolutely necessary and just filling the perforating cavity with resin material followed by crown restoration can be one of the alternative restorations.</p>