ABSTRACT
Introduction@#According to the statistical data of 2019, 10 million people in 202 countries have been registered as tuberculosis cases, 1.6 million people has died around the world. In 2019, 4,089 new tuberculosis cases were determined, of which 265 died in Mongolia.@*Purpose@#The purpose of this study is analyze quality of some drugs used in the treatment of certain groups of tuberculosis in accordance with international standards.@*Materials and methods@#Isoniazid 300 mg, Rifampicin 150 mg, Pyrazinamide 400 mg and Ethambutol 400 mg, which are commonly used in Mongolia, were analyzed according to the pharmacopoeia methods of United States, Chinese and British.@*Results@#Appearance - Complies with the requirements of the certificate for sensory examination. Identification - It was determined by an infrared spectrophotometry. Average weight - Isoniazid 547.5 mg, Rifampicin 277.6 mg, Pyrazinamide 640.8 mg, Etambutol 399.6 mg. DisintegrationIsoniazid decomposes in 2.5 minutes, Pyrazinamide decomposes in 4.6 minutes, Etambutol decomposes in 8.5 minutes. Friability – Isoniazid - 0.4%, Pyrazinamide 10.4%, Hardness – Isoniazid 0.4%, Pyrazinamide is - 49.7 N, Dissolution – Isoniazid not less than - 95.7%, Rifampicin - 78.7%, Pyrazinamide - 95.7%, Assay - Etambutol is - 399.6 mg, Rifampicin is 139.94 mg, Isoniazid is - 293.6 mg Pyrazinamide - 492.5 mg complied with the standard requirements. @*Conclusion@#Analysis of tuberculosis drugs quality by pharmacopoeia methods including its appearance, identification, average weight, friability, hardness, disintegration, dissolution and assay, the isoniazid 300 mg tablets are complied with the United States pharmacopoeia, Rifampicin 150 mg tablets met British pharmacopoeia, and Ethambutol 400 mg tablets are complied with Chinese pharmacopoeia. Pyrazinamide 400 mg tablets did not complies the friability requirements of the United States Pharmacopoeia and also satisfied with the other requirements.
ABSTRACT
Introduction: Alzheimer’s disease(AD) is the most common neurodegenerative disease and characterized by memory impairment, language deterioration, cognitive dysfunction, behavioral disturbances and deficits in activities in daily living [1,2]. Acetylcholinesterase(AchE) inhibitors have been used effective treatment for AD disease targets[3]. Therefore, it is needed to isolate bioactive compounds from plants.Materials and methods: Trisma buffer pH 8.0, DTNB(5.5-dithiobis-2-nitribenzoic acid), acethylcholinesterase enzyme, acethylcholin iodide- Ach and methanol reagents were used. Plants were collected from Mongolian khangai, steppe, gobi regions. The dried plant material were extracted with methanol then evaporiated at 400C in vacu. AChE inhibitory activity was determined using the Ellman’s colorimetric method.Calculation:A10 min-A00min= Δ A Inhibition % =100x (A10 control- Δ A sample) A10control A=absorptionResults and discussion: From the 65 samples studied, which prepared from different parts of 44 plants species, Salsola passerine, Rumex pseudonatronatus, Solidago dahurica, Chelidonium majus, Anthriscus sylvestris, Linaria buriatica and Achnatherum splendens showed more effect, their inhibition percentages were 32%, 30%, 30,7%, 35,7%, 61,5%, 29,8%, 28.8% and26.6%, respectively. Out of the plants, eight extracts were not identified AChE inhibitory activity and 30 extracts showed 10-20% weak inhibition ratios. This work represents some Mongolian plants have AChE inhibitory activity.Referens:1. Marston A., Kissling J., Hostettmann K., A rapid TLC bioautographic method for the detection of acetylcholinesterase and butyrylcholinesterase inhibitors in plants.Phytochem.Anal. 13.51- 54(2002)2. Eduardo luis Konrath., Bruna Medeiros Neves et all., Investigations of the in vitro and in vivo acetylcholinesterase and antioxidant activities of tradionally used Lycopodium species from South America on alkaloid extracts. Journal of Ethnoparmacology 139(2012)58-673. Shulz V., Ginkgo extract or cholinesterase inhibitors in dementia., Phytomedicine 10(2003)74-79