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BACKGROUND:Classical deltopectoral approach leads to a great injury to soft tissues. It is difficult to lay the steel plate on the lateral side. The steel plate is frequently laid on the anterolateral side of humerus. Fixed position is not ideal. OBJECTIVE:To evaluate the safety and feasibility of lateral deltoid-splitting approach in treatment of proximal humeral fractures. METHODS:From August 2008 to August 2013, 114 patients with fracture of surgical neck of humerus, who were treated in the Department of Orthopedics, Yan’an Hospital Affiliated to Kunming Medical University in China, were enrol ed in this study. They were treated with locking compression plate, and assigned to deltoid splitting approach group and deltopectoral approach group (n=57). Surgical trauma, fracture healing, curative effects, functional recovery and Constant shoulder score were compared between the two groups. RESULTS AND CONCLUSION:Al patients were fol owed up for at least 8 months. Compared with the deltopectoral approach group, the proximal humeral fractures were healed as scheduled in the deltoid splitting approach group, and the anteflexion, abduction, shoulder lifting of the shoulder joint, and sensation of lateral skin of the upper arm were not impacted. No significant difference in Constant shoulder score was detectable between the two groups (P>0.05), which suggested that deltoid splitting approach for treating the proximal humeral fractures was safe and feasible. In addition, in accordance with the Neer’s classification system, the excellent and good rate in the deltoid splitting approach group (91%) was significantly higher than that in the deltopectoral approach group (75%) (P<0.05). Therefore, the deltoid splitting approach can be used as the first choice for the treatment of proximal humeral fractures.
ABSTRACT
<p><b>OBJECTIVE</b>To research the cytotoxicity and in vitro antiproliferative effect of the six flavone compounds extracted from Laggera pterodonta.</p><p><b>METHOD</b>The cytotoxicity on the normal cells and antiproliferative effect on tumor cells were tested by MTT assay, and then the preliminary structure-activity relationship was analysed. The phase distribution of the cell cycle and apoptosis rate were analyzed by flow cytometry.</p><p><b>RESULT</b>The results of MTT assay showed 5,7,3',4'-tetramethoxy-3-hydroxyflavone and chrysosplenetin B inhibited growth of A549 and Hela cells significantly with a dose dependent mode, while exhibited low cytotoxicity to the two normal cells, Vero and EVC304. Both compounds contain ortho-phenolic methoxyl moietys in their structures. Flow cytometry analysis revealed that Hela cells treated with increasing quantities of chrysosplenetin B increased the percentage of cells in the G2/M phase, and Hela and A549 cells treated with increasing quantites of the 5,7,3',4'-tetramethoxy-3-hydroxyflavone and chrysosplenetin B increased the apoptosis rates.</p><p><b>CONCLUSION</b>The 5,7,3',4'-tetramethoxy-3-hydroxyflavone and chrysosplenetin B extracted from L. pterodonta showed high antiproliferative effect on cancer cells with low cytotoxicity on normal cells, and took the effects on A549 and Hela cells through the hold-up of the G2/M phase of the cell cycle and induction of the apoptosis.</p>
Subject(s)
Animals , Humans , Asteraceae , Chemistry , Cell Line , Cell Line, Tumor , Cell Proliferation , Chlorocebus aethiops , Flavones , Chemistry , Pharmacology , Flavonoids , Pharmacology , Flow Cytometry , HeLa Cells , Vero CellsABSTRACT
<p><b>OBJECTIVE</b>To study the chemical constituents of Laggera pterodonta.</p><p><b>METHOD</b>The ethanol extract of L. pterodonta was isolated by column chromatogramphy on silica gel, ODS, and Sephadex LH-20 to afford compounds. The structures of the obtained compounds were identified by chemical reactions and spectroscopic analysis.</p><p><b>RESULT</b>Nineteen compounds were separated and identified to be pterodondiol (1), ilicic acid (2), artemitin (3), chrysosplenetin B (4), 3,5-dihydroxy-3',4',6,7-tetramethoxyflavone (5), chrysosplenol D (6), 5,6,4'-trihydroxy-3,7-dimethoxyflavone (7), quercetin (8), tamarixetin (9), patuletin (10), quercetin-3-O-beta-D-galactopyranoside (11), patuletin-3-O-beta-D-glucopyranoside (12), helichrysoside (13), 4,5,7-trihydroxy-6-methoxyflavone-3-O-beta-D-rutinoside (14), kaempferol-3-O-beta-D-glucopyranoside (15), stigmasterol (16), stigmasterol 3-O-beta-D-glucopyranoside (17), 2-hydroxy-benzoic acid (18), beta-sitosterol (19).</p><p><b>CONCLUSION</b>Compounds 5, 7, 9-15, and 17-18 were isolated from this plant for the first time. The 13C-NMR data of compound 7 is reported for the first time.</p>
Subject(s)
Asteraceae , ChemistryABSTRACT
[Objective]To investigate the effects of self-contrive antomical dynamic hip lock plate(ADHLP) in fixation of intertrochanteric fracture.[Method]Seventy-three cases (male 52,female 21) of intertrochanteric fracture were treated by internal fixation with ADHLP.The age were from 19～92y,average 54.7y.Traumatic causes:traffic accident,32 cases;pedesteian tumble,21 cases;fall from height,9 cases;other reasons,11 cases. According to the evans' classification:type Ⅰ,42 cases;type Ⅱ,17 cases;type Ⅲ,9 cases;type Ⅳ,5cases.[Result]Operating time averaged 60 minutes(ranged from 40～90 minutes). Blood loss in operation:average 200 ml (ranged from 100～400 ml).Complication was not found,(such as:coxa vara;femoral head concis;rotation displacement;infection;re-fracture;lock plate broken or loosening,et al.).All cases were followed-up from 6 to 48 months,average 36 months.The function of all hips were satisfactory.The excellent and good rate was 98.6%.[Conclusion]ADHLP in fixation of intertrochanteric fracture is accord with the demands of biomechanism,The indication is widespread,applicable for osteoporotic patients or old patients mespecial.This operation is simple and reliable,with little invasive injury and less blood losing.