ABSTRACT
<p><b>OBJECTIVE</b>To prepare Dange Mingmu in-situ forming eye gel.</p><p><b>METHOD</b>The viscosity of Dange Mingmu in-situ forming eye gel was tested by adopting poloxamer 407 and 188 as thermosensitive materials, and optimizing by uniform design. Drug release in vitro was studied using a novel membraneless model. Eye irritation experiments were performed with rabbits. The duration of residence time in rabbit eyes was observed using fluorescence tracer method.</p><p><b>RESULT</b>The gelation temperature of in-situ thermosensitive gel was lowered as the P407 concentration increased. In a certain range, the gelation temperature slowly increased with the increase of P188's concentration, and the effect of P407 was greater than that of P188. The optimized concentration of P407/P188 was 19%/1%. Based the adjusted concentration, Dange Mingmu in-situ forming eye gel. was converted into freely flowing liquid below 26.9 degrees C and became gel at 34.5 degrees C after being diluted with STF. In line with zero-order kinetics, drug release in vitro depends on gel erosion. The residence time on the surface of eyes was proved to be relatively long was and nonirritant.</p><p><b>CONCLUSION</b>Uniform design is available for optimizing the formulation of thermosensitive gel for eye. The gel satisfies the requirement for ophthalmic application, and is expected to be applied in clinical practice in the future.</p>
Subject(s)
Animals , Humans , Rabbits , Drug Compounding , Methods , Drugs, Chinese Herbal , Chemistry , Pharmacology , Eye Diseases , Drug Therapy , Gels , Chemistry , Pharmacology , Ophthalmic Solutions , Chemistry , Pharmacology , Temperature , ViscosityABSTRACT
<p><b>OBJECTIVE</b>To prepare a noisome formulation of Semen Strychni alkaloids extract with high encapsulation efficiency.</p><p><b>METHOD</b>S. Strychni alkaloids were encapsulated into niosomes by pH gradient loading method. The factors influencing on the encapsulation efficiency were investigated, and formulation and preparation process of niosomes were optimised and validated.</p><p><b>RESULT</b>When the drug to lipid weight ratio was under 1: 10, pH gradient in buffer solution of citric acid at 50 degrees C was more than 4.0, the niosomes (mean diameter 179.2 nm and Zeta potential -25.41 mV) were formed with encapsulation efficiency of over 86.9%.</p><p><b>CONCLUSION</b>The pH gradient loading method was reliable for preparing niosomes of Semen Strychni alkaloids extract.</p>
Subject(s)
Alkaloids , Chemistry , Capsules , Drug Compounding , Methods , Drugs, Chinese Herbal , Chemistry , Hydrogen-Ion Concentration , Liposomes , Chemistry , Strychnos nux-vomica , Chemistry , TemperatureABSTRACT
<p><b>OBJECTIVE</b>To study the preparation of cantharidin entrapped non-ionic surfactant vesicle (noisome)and evaluate its quality.</p><p><b>METHOD</b>The niosome loaded with cantharidin was prepared using injection method by non-ionic surfactants as the carrier. An centrifugation separation method and HPLC analysis method of the cantharidin were established to detect the entrapment efficiency. The optimum preparation technology was established by a orthogonal experiment. The morphology, and particle size were studied to evaluate the preparation.</p><p><b>RESULT</b>The average size of niosomes were (209. 8 +/- 0.5) nm. The entrapment efficiency of the CTD-NS was (27.5% +/- 2.0%) and Zeta potential was (41.5 +/- 0.65) mV.</p><p><b>CONCLUSION</b>The preparation of cantharidin noisome by TweenA and SpanB is practicable and successful. These experiments can be the basement of developing targeting drug delivery system.</p>
Subject(s)
Cantharidin , Chemistry , Chromatography, High Pressure Liquid , Drug Delivery Systems , Liposomes , Chemistry , Particle Size , Surface-Active AgentsABSTRACT
<p><b>OBJECTIVE</b>To prepare silymarin nanosuspension and lyophilized power for enhancing the dissilution of poorly soluble drugs.</p><p><b>METHOD</b>The precipitation technique was adapted to produce the silymarin nanosuspensions respectivly applying Tween 80, SDS and Poloxamer188 as stabilizers. The lyophilized formula contained 5% mannitol as cryoprotectant. Particle size, Polydispersity index and Zeta potential were detected by Mastersizer nano ZS (Malvern England). Morphological character was observed with Transmission Electron Microscopy. The product's structure was performed with X-ray diffractometer.</p><p><b>RESULT</b>The silymarin nanosuspension was successfully prepared, in which the drug particle size was about 100-300 nm,and the particles had ball-like shape and good dispersive properties.</p><p><b>CONCLUSION</b>This study provided potential for the neotype dosage form development of the Chinese Traditional Medicine.</p>