ABSTRACT
<p><b>OBJECTIVE</b>To establish an instant determination method of citrinin in red kojic by high performance capillary electrophorphores for the first time.</p><p><b>METHOD</b>Red kojic was extracted with the mixtrue of Toluene and ethyl acetate (70:30). Separation was carried out in an uncoated fused silica capillary (50 microm x 45.0 cm). Meanwhile, a running voltage 15 kV, 20 mmol L(-1) borax buffer with 10.0 mmol L(-1) sodium deoxycholate (pH 9.3) and a UV detector at 212 nm were adopted.</p><p><b>RESULT</b>Regression equation of citrinin was Y=9434 + 16781X (r =0.990), The lower limit of quantification (S/N > or =3) was 0.5 mg mL(-1). The assay coefficients of variation ranged from 98.8% to 101.1%. The intra and inter recovery ranged from 0.83 to 1.54% and from 1.86 to 5.09%. Twenty samples were determined with the method.</p><p><b>CONCLUSION</b>The method is proved to be simple, rapid and accurate, and it can be used to determine citrinin in red kojic.</p>
Subject(s)
Citrinin , Electrophoresis, Capillary , Methods , Hydrogen-Ion Concentration , Monascus , Chemistry , Reproducibility of ResultsABSTRACT
<p><b>AIM</b>To design and synthesize new phenyloxyisobutyric acid analogues as antidiabetic compounds.</p><p><b>METHODS</b>Eight new target compounds were synthesized by combination of lipophilic moieties and acidic moiety with nucleophilic replacement or Mitsunobu condensation. The eight compounds were confirmed by 1H NMR, 13C NMR, IR and MS.</p><p><b>RESULTS</b>In vitro insulin-sensitizing activity (3T3-L1 adipocyte) demonstrated, that the cultured glucose concentration of up-clear solution detected with GOD-POD assay were 5.942, 6.339, 6.226 and 6.512 mmol x L(-1), respectively, when rosiglitazone, pioglitazone, compounds A and B were added to the insulin-resistant system.</p><p><b>CONCLUSION</b>In vitro insulin-sensitizing activity of target compound A is in between that of rosiglitazone and pioglitazone, and activity of target compound B is slightly less than that of pioglitazone.</p>
Subject(s)
Animals , Mice , 3T3-L1 Cells , Adipocytes , Butyrates , Chemistry , Pharmacology , Hypoglycemic Agents , Chemistry , Pharmacology , Insulin , Pharmacology , Molecular Structure , PPAR gamma , PharmacologyABSTRACT
<p><b>AIM</b>To study the chemical constituents of Pseudotsuga sinensis Dode (Pinaceae).</p><p><b>METHODS</b>To separate the constituents of P. sinensis by using various kinds of chromatography and identify their structures on the basis of spectral analysis.</p><p><b>RESULTS</b>Six compounds were isolated from P. sinensis. Their structures were established as 5,7,4'-trihydroxy-6-methylflavanone (poriol, I), 3,5,7,3',4'- pentahydroxyflavonone (quercetin, II), 5,7,3',5'-tetrahydroxy-6- methylflavanone (III), 5,7,3',5'-tetrahydroxyflavanone (IV), 3,5,7,3',5'-pentahydroxyflavanone (V) and 5-hydroxy-6-methylchromone-7-O-beta-D-glucopyranoside (VI) based on the analysis of spectral data of IR, UV, MS, 1D and 2D-NMR.</p><p><b>CONCLUSION</b>Compounds III and VI are new compounds. All of six compounds were isolated from this plant for the first time.</p>
Subject(s)
Chromones , Chemistry , Flavanones , Chemistry , Glucosides , Chemistry , Molecular Structure , Plant Bark , Chemistry , Plants, Medicinal , Chemistry , Pseudotsuga , Chemistry , Quercetin , ChemistryABSTRACT
<p><b>OBJECTIVE</b>To study the chemical constituents from the whole plant of Aster poliothamnus.</p><p><b>METHOD</b>Separating the chemical constituents by means of chromatography and identifying ther structures on basis of chemical and spectral tecnology.</p><p><b>RESULT AND CONCLUSION</b>A new triterpene saponin was isolated and identified.</p>