ABSTRACT
It is very important to prevent hemolytic transfusion reaction. We experienced 1 case of transfusion in liver transplantation patient with multiple unexpected antibodies. On crossmatching for 20 pRBC (packed red blood cells), weak reactions were observed in room temperature, but strong reactions were observed in 37 , and AHG (antihuman globulin) phase. The patient had unexpected antibodies of anti-C, anti-Jka, anti-Dia. The probabilitis of compatible blood of anti-C, anti-Jka, anti-Dia in Koreans had been reported to be 14.2%, 32.8%, and 90%, respectively. We received 217 units of C antigen-negative pRBCs prepared by typing of 2679 type B pRBCs from Central, Eastern and Southern Blood Center of the Korean Red Cross. From the 217 units, 60 units of pRBCs compatible with patient's serum in AHG phase were prepared in our laboratory. The hemolytic tranfusion reaction can be successfully prevented by aid of Blood Center of Korean Red Cross and it should be emphasized that performing crossmatching including AHG phase is very important.
Subject(s)
Humans , Antibodies , Blood Group Incompatibility , Liver Transplantation , Liver , Red Cross , TransplantationABSTRACT
Some variables can influence the efficiency of leukocyte removal filter for red cell concentrates. We performed this study to evaluate whether the filtration efficiency for platelet concentrates(PCs) can be influenced by bedside filter(PXL8K, Pall Corporation, USA) used, platelet storage time and transient stopping of platelet agitation, and to measure the amount of cytokines released from donor leukocytes in PCs during the storage time. In addition, we examined the effect of leukocyte reduction by filtration before the storage of PCs on the subsequent generation of cytokines. Any of the above mentioned variables did not make significant differences. From day 1 to day 5, there were significant increases in IL-1 beta (<1 vs. 8.6 pg/mL, p<0.05), IL-8 (<10 vs. 455.3 pg/mL, p<0.05) and TNF-alpha (<4 vs. 16.6 pg/mL, p<0.05) in unflltered PCs, whereas no increased revels of cytokines were found in the cases of prestorage filtration.
Subject(s)
Humans , Blood Platelets , Cytokines , Dihydroergotamine , Filtration , Interleukin-1beta , Interleukin-8 , Leukocytes , Tissue Donors , Tumor Necrosis Factor-alphaABSTRACT
No abstract available.