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Search for endophytic diazotrophs in barley seeds

S. Zawoznik, Myriam; C. Vázquez, Susana; Díaz Herrera, Silvana M.; D. Groppa, María.

Braz. j. microbiol ; 45(2): 621-625, Apr.-June 2014. ilus, tab

Article in English | LILACS | ID: lil-723126

ABSTRACT

Eight endophytic isolates assigned to Pseudomonas, Azospirillum, and Bacillus genera according to pheno-genotypic features were retrieved from barley seeds under selective pressure for nitrogen-fixers. Genetic relationships among related isolates were investigated through RAPD. Six isolates displayed nitrogen-fixing ability, while all could biosynthesize indolacetic acid in vitro and showed no antibiosis effects against Azospirillum brasilense Az39, a recognized PGPR.

Subject(s)

Azospirillum brasilense/isolation & purification , Bacillus/isolation & purification , Endophytes/isolation & purification , Hordeum/microbiology , Nitrogen Fixation , Pseudomonas/isolation & purification , Seeds/microbiology , Antibiosis , Azospirillum brasilense/classification , Azospirillum brasilense/genetics , Azospirillum brasilense/metabolism , Bacillus/classification , Bacillus/genetics , Bacillus/metabolism , DNA, Bacterial/chemistry , DNA, Bacterial/genetics , DNA, Ribosomal/chemistry , DNA, Ribosomal/genetics , Endophytes/classification , Endophytes/genetics , Endophytes/metabolism , Indoleacetic Acids/metabolism , Molecular Typing , Pseudomonas/classification , Pseudomonas/genetics , Pseudomonas/metabolism , Random Amplified Polymorphic DNA Technique , /genetics , Sequence Analysis, DNA

ABSTRACT

Subject(s)

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