ABSTRACT
Objective Paclitaxel and nanosilver were co-encapsulated into folic acid-albumin nanoparticles to increase the toxicity and uptake of the drug and improve the targeting ability of tumor that highly expressed folic acid (Fa) receptor. Methods The mean Fa binding number of bovine serum albumin (BSA) was determined by ultraviolet absorption method. The nanoparticles were prepared through self-assemble method and then its shape, partical size, Zeta potential, and encapsulation efficiency were characterized with the dynamic light scattering (DLS) and transmission electron microscope (TEM). In addition, the cellular uptake of nanoparticles and the in vitro anti-tumor effect of drug-loaded nanoparticles were investigated on the KB cells model of oral epithelial carcinoma. Results Ultraviolet absorption results showed that the average Fa binding number of Fa-BSA was 11. The nanoparticles were discrete and uniform spheres with the average size of (98.20 ± 3.58) nm and Zeta potential of (-39.90 ± 1.98) mV. Cellular uptake experiments showed that folate-modified albumin nanoparticles were more easily taken up by KB cells. Cytotoxicity and apoptosis assay indicated that the modification of folic acid and co-loaded nanosilver could increase the inhibition of tumor cell proliferation and promote KB cells apoptosis. Conclusion The prepared nanoparticles have small particle size and high encapsulation efficiency, which can enhance the ability of the nanoparticles to be uptaken and the toxicity of the drug-loaded nanoparticles against tumor cells.
ABSTRACT
In this review, the ability of borneol and menthol as the common penetration enhancers in traditional Chinese medicines was summarized, and the effect of borneol and menthol on the ability of nanopreparations across the main physiological barriers of the human body promoting drug absorption or increasing the distribution of drugs was also reviewed, which aims to provide reference for promoting the treatment effect of nanopreparations through the combination of traditional Chinese medicine and modern preparation technology.
ABSTRACT
Nanotechnology has broad prospects and great potential in improving the druggability of active ingredients of Chinese materia medica (CMM). This article systematically summarized the methods and advantages of nanotechnology in improving oral absorption, changing the distribution in the body, increasing the targeting ability, and improving transdermal and mucous membrane absorption of active ingredients of CMM. In addition, we analyzed the problems in the development of improving the druggability of active ingredient of CMM by nanotechnology, put forward the strategies for solving these problems, and discussed the research prospects.