1.
Acta physiol. pharmacol. ther. latinoam
;
46(2): 144-8, 1996. ilus
Article
in English
| LILACS
| ID: lil-172320
ABSTRACT
In the present study we describe a non-enzymatic technique for the isolation of rat hepatocytes by perfusion of liver through portal vein. The perfusion media consist of 1 mM sodium percholorate. 5 mM sodium citrate, 10 mM glucose, 129 mM NaCl and 0.1 per cent bovine-serum albumin at pH 7.4. After purification through diatrizoate gradient, electron microscopical studies revealed that most of purified hepatocytes were well preserved and presented a normal ultrastructure, thus correlating with previous biochemical results. The present method enables the recovery of metabolically and morphologically normal hepatocytes.
Subject(s)
Animals , Male , Rats , Citrates , Liver/ultrastructure , Perchlorates , Cell Separation/methods , Sodium Compounds , Cell Survival , Centrifugation, Density Gradient , Diatrizoate , Perfusion , Rats, Wistar
2.
Acta physiol. pharmacol. latinoam
;
34(4): 343-54, 1984.
Article
in English
| LILACS
| ID: lil-24640
3.
Acta physiol. pharmacol. latinoam
;
34(4): 355-66, 1984.
Article
in English
| LILACS
| ID: lil-24641
Subject(s)
Male , Animals , Mice , Rats , Insulin , Liver , Receptor, Insulin , Binding, Competitive
4.
Acta physiol. pharmacol. latinoam
;
34(4): 427-40, 1984.
Article
in English
| LILACS
| ID: lil-24648
5.
Acta physiol. pharmacol. latinoam
;
31(1): 13-24, 1981.
Article
in English
| LILACS
| ID: lil-6385
6.
Acta physiol. pharmacol. latinoam
;
31(4): 217-28, 1981.
Article
in English
| LILACS
| ID: lil-6406
7.
Acta physiol. pharmacol. latinoam
;
31(4): 229-33, 1981.
Article
in English
| LILACS
| ID: lil-6407