ABSTRACT
The synthesis of histone mRNA is closely coupled with DNA replication. During the S-phase of the cell cycle, the level of histone mRNA increases over fifty fold then rapidly disappears at the start of G2-phase. The presence, therefore, of abundant quantities of histone mRNA provides a molecular marker of cycling cells. The expression of histone mRNA was investigated by in situ hybridization method in N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN) induced rat bladder tumor to determine the proliferation patterns. This method resulted in intense brown/black cytoplasmic staining of cells containing histone mRNA and extent and intensity of stain were increased in tumorigenesis (normal, simple hyperplasia, nodular or papillary hyperplasia, and transitional cell carcinoma-Ta,T1). The higher S-phase fraction indicated greater biological malignancy based on the fact that the extent and intensity of stain progressively increased with tumorigenesis.
Subject(s)
Animals , Rats , Carcinogenesis , Cell Cycle , Cytoplasm , DNA Replication , Histones , Hyperplasia , In Situ Hybridization , RNA, Messenger , Urinary Bladder Neoplasms , Urinary BladderABSTRACT
We measured the concentration of serum PSA to define statistical upper limits for PSA in relation to age in 228 men without clinical evidence of prostatic cancer. We defined the cancer-free group as men with a PSA less than or equal to 2.5ng/ml(ELSA-PSA' monoclonal immunoradiometric assay) and nonsuspicious digital rectal examination and those with an abnormality in either parameter underwent transrectal ultrasound and pathologically excluded prostatic cancer from the group by transrectal prostatic biopsy or transurethral resection. A total 252 men between 40 and 79 years of age entered the protocol, of whom 228 men satisfied our cancer-free criteria. There was a significant correlation between natural logarithm transformed PSA(In PSA) and age by linear regression analysis(r=0.32, p=0.02) and age-specific upper limits of PSA=mean+2SD) were 2.62ng/ml in the 40 to 49-year group, 3.45ng/ml in the 50 to 59-year group, 5.09ng/ml in 60 to 69- year group and 7.07ng/ml in the 70 to 79-year group.
Subject(s)
Humans , Male , Biopsy , Digital Rectal Examination , Linear Models , Prostatic Neoplasms , UltrasonographyABSTRACT
A study was carried out to demonstrate that Colonlyte(polyethylene glycol electrolyte lavage solution; PEG-ELS), a colonic lavage solution presently used for gut cleansing in preparation for colonic procedures, can be an effective agent in the preoperative bowel preparation for urologic surgery. Eighteen patients received 1-day preparation with 4,000ml of Colonlyte. Patient tolerance evaluated by a questionnaire showed overall comfortness in 77.8% of the patients. There was no electrolyte changes between pre- and post-preparation. Bowel cleansing was adequate(""good"" to ""excellent"" cleansing) in 89 % of the patients. Gas passage was identified 3 days and 9 hours postoperatively(mean) after termination of general anesthesia. One patient (5.6%) developed postoperative superficial wound infection but there was no serious complications such as intra-abdominal abscess, anastomotic disruption, or sepsis. Colonlyte is a safe, effective, and economic method of preoperative bowel preparation in urologic surgery.
Subject(s)
Humans , Abdominal Abscess , Anesthesia, General , Colon , Surveys and Questionnaires , Sepsis , Therapeutic Irrigation , Wound InfectionABSTRACT
Congenital vaginal atresia is rare and occurs as a result of aplasia of the mullerian ducts This is a source of great embarrassment and distress to patients and their close relatives. These patients are usually treated with chronic dilatation of the shallow vaginal introitus (Frank Technique) or by skin graft vaginoplasty (McIndoe procedure). Neither of these procedures is entirely satisfactory, as both may lead to neovaginal stenosis, inadequate length or poor lubrication. The use of an isolated segment of intestine for vaginoplasty generally circumvents these problems. A 31-year-old unmarried woman had suffered from vaginal narrowing and stenosis after skin graft vaginoplasties. We treated her with vaginoplasty using an isolated ileal segment.
Subject(s)
Adult , Female , Humans , Constriction, Pathologic , Dilatation , Ileum , Intestines , Lubrication , Mullerian Ducts , Single Person , Skin , TransplantsABSTRACT
Simple cysts of the testis are rare benign lesions of uncertain etiology. A 3-month-old male infant presented with painful enlargement of the left testicle and was suspected for testicular torsion. On scrotal exploration, the diagnosis of intratesticular simple cyst was made intraoperatively and the patient treated with orchiectomy.
Subject(s)
Humans , Infant , Male , Diagnosis , Orchiectomy , Spermatic Cord Torsion , TestisABSTRACT
A new data management system for urologic patient was designed by composing revised discharge summary and a computer program. The revised .discharge summary consists of more detailed clinical records and maintenance of clinical records during the follow-up period post discharge. The computer program was written in FoxPro Version 2.0 and the urologic code system was developed by applying some modifications on the international classification of diseases. The present program takes major advantages in easy and errorless entry and retrieval of data. These include pull-down menus using simultaneous input and coding system without any needs of typing the required codes for problem, management, and diagnosis. Data search and case statistics can be easily obtained with any combinations of problem, management, and diagnosis. The authors consider the present system, in which the revised discharge summary and computer program were closely linked, is very useful in patient data management.