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Objective@#To assess the clinical characteristics and identify the risk factors in the acute myocardial infarction (AMI) patients complicating with ventricular septal rupture (VSR).@*Methods@#A retrospective study was performed on 96 AMI patients complicating with VSR, who were hospitalized in the Second Xiangya Hospital of Central South University, Hunan Provincial Peoples′ Hospital, the First Affiliated Hospital of University of South China, the Second Affiliated hospital of University of south China, Xiangtan Central Hospital from December 2007 to May 2017. There were 46 females and the age was (66.2±10.7) years (from 43 to 90 years). Patients were divided into in-hospital survival group (n=64) and in-hospital death group (n=32). The 96 patients were also divided into the early death group (survived ≤2 weeks after admission, n=50) and non-early death group (survived>2 weeks after admission, n=46). Multivariate logistic regression was used to analyze the independent risk factors of the early death.@*Results@#Location of VSR was available in 71 patients, VSR was located at the apical or anterior septum near the apical region in 64.0% (32/50) patients with the anterior AMI, VSR was located at the posterior wall and basal inferior segment in 57.1% (12/21) patients with non-anterior AMI. Compared to the in-hospital survival group, patients in the in-hospital death group were older ((69.6±11.3) years vs. (64.6±10.1) years, P=0.031), incidence of non-ventricular aneurysm (71.9% (23/32) vs. 37.5% (24/64), P=0.001) and anterior AMI (84.4%(27/32) vs. 62.5%(40/64), P=0.028) was significantly higher in the in-hospital death group than in the in-hospital survival group. The comparison between the early death group and non-early death group showed that older age, female, no history of angina or myocardial infarction, Killip grade>Ⅲ, and non-ventricular aneurysm were related to increased risk of the early mortality in this patient cohort. Logistic regression analysis revealed that female (OR=5.109,95%CI 1.19-22.00, P=0.012), no history of angina or myocardial infarction (OR=23.34, 95%CI 3.44-158.37, P=0.001), Killip grade>Ⅲ(OR=5.35, 95%CI 1.26-22.66, P=0.019) and non-ventricular aneurysm (OR=6.30,95%CI 1.67-23.73, P=0.005) were independent risk factors for early death in this patient cohort.@*Conclusion@#The risk factors of in-hospital death include older age, non-ventricular aneurysm and anterior AMI. Female, no history of angina or myocardial infarction, Killip grade>Ⅲ and non-ventricular aneurysm are independent risk factors for the early death of AMI patients complicating VSR.
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Objective To investigate the effect of kinesio taping on upper crossed syndrome (UCS). Methods From December, 2016 to March, 2017, 52 patients with UCS were randomly divided into control group (n=26) and observation group (n=26). The control group re-ceived exercise therapy, and the observation group received kinesio taping in addition, for four weeks. They were assessed with Visual Ana-logue Scale (VAS), forward head angle (FHA) and forward shoulder angle (FSA). Results There was no significant difference in the score of VAS, FHA and FSA between two groups before treatment (P>0.05). After treatment, the scores of VAS, FHA and FSA significantly de-creased (t>2.804, P2.632, P<0.05). Conclusion Exercise therapy could relieve pain and correct abnormal posture of UCS, which is more effective combined with kinesio taping.
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Objective To establish a novel multiplex amplification system which comprises 24 Y-STR loci.Methods Total 24 Y-STR gene loci, concluding DYS531,DYS630,DYS622,DYS552,DYS510, DYS449, DYS459a/b, DYS446, DYS443, DYS635, DYS587, DYS527a/b, DYS460, Y-GATA-A10, DYS520, DYS557,DYS522,DYS481,DYS570,DYS385a/b,DYS444, were chosen for establishing the fluorescence multiplex amplification system. The specificity, identity, sensitivity, balance of the amplification, anti-in-terference and accuracy of the system were detected and the gene diversity was investigated in the popu-lation of Guangdong.Results No band was found in nonhuman and female samples that were tested by the established multiplex amplification system. The same genotyping results were obtained from different tissues of the same person. Complete profiles could be obtained from more than 0.1 ng of the standard sample 9948. The loss of alleles was found when the common inhibitors such as hemoglobin and calci-um ion were added 120-200μmol/L and 1.5-2.0 mmol/L respectively to the system which with a strong anti-interference to the indigo, humic acid and EDTA. The typing of 24 Y-STR system could give the reliable results when 146 unrelated male individuals were detected and compared with the Yfiler system parallelly. The haplotype diversity(HD)of the population in Guangdong reached 0.99972 that was better than the result retained from Yfiler system, which the HD was 0.99858.Conclusion The fluorescence amplification system with 24 Y-STR loci established in present study has a wildly application prospect and can be used for cases inspection, paternity tests and Y-STR database construction.
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<p><b>OBJECTIVE</b>To explore effects and potential mechanisms of high insulin environment on high density lipoprotein (HDL) generation-related functional protein ABCA1.</p><p><b>METHODS</b>[(3)H] labeled cholesterol efflux from mature 3T3-L1 adipocytes was detected by liquid scintillation counting. ABCA1 mRNA and protein expression in mature 3T3-L1 adipocytes post stimulation with various concentrations of insulin was detected by real-time fluorescence-based quantitative techniques and Western blot, respectively, in the absence and presence of CHX (cycloheximide, CHX), calpeptin (calpain pathway inhibitor) or MG-132 (proteasome pathway inhibitor).</p><p><b>RESULTS</b>Cholesterol efflux rates were reduced post insulin stimulation in a dose-dependent manner ((7.06 ± 0.27)%, (6.59 ± 0.30)%, (6.34 ± 0.24)%, (5.07 ± 0.40)%, and (4.71 ± 0.40)% at 0, 1, 10, 10², and 10³ nmol/L of insulin, P < 0.05). Cholesterol efflux rates decreased in a time-dependent manner post 10³ nmol/L insulin stimulation (6.52 ± 0.30)%, (5.59 ± 0.71)%, (5.44 ± 0.37)%, (4.52 ± 0.32)%, and (4.38 ± 0.33)% at 0, 2, 4, 6, 12 h, respectively). ABCA1mRNA levels were not affected by insulin (P > 0.05). ABCA1 protein level was significantly downregulated in 10³ nmol/L insulin group compared to 0 nmol/L insulin group (P < 0.01). Compared with the 0 h group, ABCA1 protein level was significantly reduced in 6 h group (P < 0.05) and further reduced in 12 h group (P < 0.01). Both calpeptin and MG-132 could partly reduce insulin-induced degradation of ABCA1. Compared with the negative control group, ABCA1 protein levels were significantly upregulated by cotreatment with calpeptin and MG-132, respectively (both P < 0.01).</p><p><b>CONCLUSION</b>Our data suggest that high insulin level could promote the ABCA1 protein degradation and reduce cholesterol efflux from mature 3T3-L1 adipocytes through calpain and proteasome pathway, thus, produce a circumference not suitable for nascent HDL formation in 3T3-L1 adipocytes.</p>
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Animals , Mice , 3T3-L1 Cells , ATP Binding Cassette Transporter 1 , Adipocytes , Calpain , Insulin , Leupeptins , Lipoproteins, HDL , Proteasome Endopeptidase Complex , RNA, MessengerABSTRACT
<p><b>OBJECTIVE</b>To investigate the polymorphisms of 17 Y-STR loci in Han population in Guangdong Province and explore its application in forensic medicine.</p><p><b>METHOD</b>Seventeen Y-STR loci (DYS19, DYS385a/b, DYS635, DYS389I, DYS389II,DYS390, DYS391, DYS392, DYS393, DYS437, DYS438, DYS439, DYS448, DYS456, DYS458, Y-GATA-H4) were analyzed using Y filer™ kit in 1000 unrelated individuals and 1000 DNA-confirmed father-son pairs. All mutations were confirmed by DNA sequence analysis.</p><p><b>RESULTS</b>No shared haplotypes were observed among the 1000 unrelated male individuals, and the gene diversity of 17 Y-STR loci ranged from 0.4285 (DYS391) to 0.9654 (DYS385a/b). The samples yielded an overall haplotype diversity of 1.000. A total of 17000 meiotic events were investigated in 1000 father-son pairs with DNA-confirmed biological paternity and 46 differences were observed between the fathers and sons. The average mutation rate was 0.0027 (95%CI, 0.0020-0.0036) per locus.</p><p><b>CONCLUSION</b>The 17 Y-STR loci included in Y-filer have high genetic polymorphisms in South China Han population and have good prospect for application in forensic medicine.</p>
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Humans , Male , China , Chromosomes, Human, Y , Genetics , Ethnicity , Genetics , Gene Frequency , Haplotypes , Polymorphism, GeneticABSTRACT
Objective To analyze the variations of glycerol-3-phosphate dehydrogenase 1 like gene (GPD1-L) and address the association with sudden m anhood death syndrom e (SMDS). Methods The genom ic DNA was extracted from blood sam ples of the SMDS group and the norm alcontrolgroup.The exons, exon-in-tron boundaries and 3′-U TRs of coding region of GPD1-L w ere PCRam plified and DNAsequenced di-rectly to confirm the types of variations. The genotype frequency and allele frequency w ere analyzed statistically. Results There w ere tw ovariants in the SMDS group, c.465C>Tand c.*18G>T, the latter existed certain degree difference of genotype distribution and allele frequency betw een the SMDS group and the control group, but there was no statistically significant (P>0.05). Conclusion The relation be-tw een gene m utation of GPD1-L and the occurrence of Chinese SMDS deserves a further research.
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Objective To study the application of a new magnetic beads made in China in DNA extraction of forensic biological samples with automation workstation.Methods DNA was extracted from common forensic biological samples by QIAGEN Bio-Robert Universal System and a new magnetic beads made in China,and then typed with Identifiler system in ABI3130XL Genetic Analyzer.210 of these samples were also quantitated by ABI7500 Real Time System.Results Total of 9100 genomic DNA was extracted from various forensic biological samples by the new magnetic beads made in China and automation workstation methods,and most of them were successfully typed for STR analysis.In these biological samples,oral swabs and muscles were of the highest Success rate of STR typing(100%),and the lowest was touched cell samples (50.0%).Conclusion The new magnetic beads made in China with automation workstation methods can be applied to DNA extraction of most forensic biological samples.
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Objective To investigate the polymorphism of seventeen Y chromosomal short tandem repeat(STR) loci in Dong,Miao and Zhuang nationality in Guangxi,China.Then to evaluate their forensic application.MethodsDNA was extracted from oral swab of minority population with magnetic beads method and then seventeen Y-STR loci were amplified with AmpFlSTR○R YfilerTM PCR Amplification Kit System.The PCR products were detected with 3l30XL Genetic Analyzer.The allele frequencies,diversity and genetic distance were calculated.Results The information of allele distribution and gene diversity of the 17 Y-STR loci were obtained from three minority population.Total 275 haplotypes were found in three minority population.The haplotype diversity of Dong nationality is 0.9972,Miao is 0.9966,Zhuang is 0.9964.The genetic distance between Dong and Miao nationality is most close.Conclusion The haplotype diversity of 17 Y-STR loci is quite sensitive in racial identification,paternity identification and human population genetics.
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AIM: To observe the effects of valsartan on the cardiac hypertrophy and the expression of PTEN in spontaneously hypertensive rats (SHR). METHODS: Twenty 12-week-old male SHR were randomly divided into 2 groups:SHR positive control group and valsartan treating group ( 30 mg?kg -1?d -1). 10 homogenous Wistar-kyoto (WKY) rats were served as normal control group. Blood pressure and the ratio of left ventricular weight to body weight(LVW/BW)of rats were monitored periodically during the 8-weeks studies. Expression of PTEN in cardiac myocytes was determined by immunohistochemistry. RESULTS: Blood pressure and LVW/BW increased in the valsartan group more than those in the SHR control group, and the expression of PTEN in cardiac myocytes in valsartan group increased more than that in the SHR control group, but the indexes were lower than those in the WKY group. CONCLUSION: Valsartan can not only inhibit the progression of cardiac hypertrophy in spontaneously hypertensive rats, but also increase the expression of PTEN. PTEN may play a role in cardiac hypertrophy.
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[ Objective ] To supply a technological evidence for the rapid reproduction of Andrographis paniculata (Burm. f.) Nees (APN). [Methods] Different explants from sterile seedling of Andrographis paniculata (Burm. f.) Nees were cultured in vitro to induce the production of fascicular buds. [ Results ] Cotyledon with nodes and half cotyledon with nodes were the most suitable explants for culture, and the budding rate was 100% and over 90% respectively. 6-Benzylaminopurine (BA) in the concentrations of 0.5 ~ 1.0mg/L were beneficial for the reproduction and growth of buds. The age of explants had no correlation with budding. The optimal rooting medium was MT (Murashige and Tucher) medium added with Img/L NAA (naphthaleneacetic acid) or IBA (indolebutyric acid). A highest rooting rate as much as 61.9% was achieved after 10 days of culture. [Conclusion] An effective plant regeneration system has been established for explants of Andrographis paniculata (Burm. f.) Nees.
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Objective To investigate the regulation effect of irbesartan on the expression of angiotensin-converting enzyme 2 (ACE2) mRNA in the kidney of spontaneously hypertensive rats (SHR). Methods Twenty 14-week-old male SHR were randomly divided to SHR group (n=10) and irbesartan group (n=10); ten 14-week-old male WKY group were served as control. Irbesartan group was given a daily dose of 50 mg/kg irbesartan for 12 weeks by gavage and the other two groups were fed with a normal diet. ACE2 in kidney was examined by immunohistochemistry and RT-PCR. Plasma angiotensin II in each group were estimated by radioimmunoassay (RIA). Results The ACE2 mRNA expression was significantly down-regulated in the kidney of SHR group compared with WKY group (0. 72?0. 11 vs 1. 11?0. 15). After 12 weeks treatment with irbesartan, the attenuated ACE2 expression in irbesartan group was markedly enhanced compared with SHR group (1. 03?0. 13 vs 0. 72?. 0. 11). ACE2 mRNA expression in SHR kidney was correlated significantly with plasm angiotensin II level. (r = 0. 83, P
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AIM: To investigate the expression of angiotensin-converting enzyme 2 (ACE_2) in the renal of spontaneously hypertensive rats and the effects of angiotensin type-1 receptor blockade. METHODS: Twenty of 14-week-old male SHR were randomly divided into two groups (n=10 in each): SHR group and irbesartan group. Ten of 14-week-old male WKY group were served as control. Irbesartan group were given a daily dose of 50 mg?kg -1 irbesartan for 12 weeks by gavage and the other two groups were fed with a normal diet. Angiotensin-converting enzyme 2 was examined by immunohistochemistry and RT-PCR. RESULTS: ACE_2 in the renal was significantly down-regulated in the SHR group compared with the WKY group ( 0.72? 0.11 vs 1.11? 0.15). After 12 weeks treatment with irbesartan, the attenuated ACE_2 expression in irbesartan group was markedly enhanced compared with the SHR group ( 1.03? 0.13 vs 0.72? 0.11). CONCLUSION: Hypertension induces the reduction of ACE_2 in the renal in rats and irbesartan markedly increases ACE_2 expression. It suggests that up-regulation ACE_2 which is one of counter-regulatory particular components in the renin-angiotensin system may be a new mechanism of curing hypertension beyond blockade angiotensin type-1 receptor.
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Objective To investigate the level changes of plasma soluble intercellular adhesion molecule-1(sICAM-1), endothelin(ET) and Nitric oxide(NO) and the effects of perindopril on their levels in elderly patients with essential hypertension. Methods Enzyme linked immunosorbent assay(ELISA), radioimmunoassay and nitrate reductase method were used to respectively measure the plasma levels of sICAM-1, ET and NO in 63 elderly patients with mild to moderate essential hypertension before and after perindopril treatment and 40 normotensive elderly individuals. Results Elderly hypertensive patients exhibited significantly higher plasma levels of sICAM-1 and ET, and lower plasma level of NO than normotensive elderly individuals did(P
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Objective To study the genetic polymorphism of 15 STR loci of 3 populations in Guangxi province. Methords DNA samples of unrelated individuals from 314 Gelao population,332 Mulao population,238 Yao population were analyzed using AmpFlSTR IdentifilerTM PCR Amplification Kit and 3100 Genetic Analyzer. Results The matching probability of the 15 STR loci was 1.839 ?10-16 ~ 5.073 ? 10 -17 and combined paternity of exclusion was 0.9999983 ~ 0.9999991 in the 3 populations. Conclusion The results showed that the 15 STR loci in Identifiler?PCR Amplification systems were useful for forensic case works in Gelao population, Mulao population and Yao population .
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Objective To study the quality standard of Jinyou Tankeqing Tablets (JTT). Methods Total flavonoids in JTT were identified by TLC. Naringin content in JTT was determined by HPLC. Results The relevant spots of total flavonoids in JTT can be identified by TLC. The content of naringin in JTT can be determined by HPLC. The linearity of naringin was good in the range of 2～20 ?g (r= 0.999 9). The average recovery of naringin was 101.29 %with RSD = 2.58 %. Conclusion The established quality standard is simple,feasible and repeatable,and can be used for the quality supervisory of JTT.
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Objective To optimize the extraction method for assaying Jinyou Tankeqing Tablets(JTT).Methods With the total flavonoids content as the index,Orthogonal Experimental Design was used to optimize the extraction condition for assaying JTT.Results The optimized ultrasonic extraction method was as follows: adding 0.2 mL?mg-1 powder to 95 % ethanol,extracting for 30 min;the optimized Sohlex extraction method was: adding 1.2 mL?mg-1 powder to 95 % ethanol,extracting for 2 h,the former method being better than the later.Conclusion The optimized extraction method for assaying JTT is adding 0.2 mL?mg-1 powder to 95 % ethanol and extracting for 30 min with ultrasonic wave.
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AIM: To study the expression of transforming growth factor ? 1(TGF ? 1) in the nephric tubule of spontaneous hypertensive rats and the relation of the TGF ? 1 and the renal tubulointerstitial fibrosis, and to investigate the effect of losartan on this expression and the protection effects of it on the kidney. METHODS: Same age male WKYs and SHRs were involved in this experiment. SHRs were divided into positive control (SHR C) group and losartan treatment (SHR L) group. The artenia pressure, renal function and ? 2 MG of mice was monitored before and after experiment and the expression of TGF ? 1 in nephric tubule were measured by immuonohistochemitry assay. RESULTS: Compared with the WKY group, the contents of ? 2 MG markedly increased in SHR C group (P 0.01 ). No or a few expression of TGF ? 1 occurred in WKY group. The expression of TGF ? 1 significantly increased in the positive control group with the progression of the hypertensive course, and meantime, the expression of TGF ? 1 significantly decreased in the losartan treatment group and the blood pressure also decreased. CONCLUSION: As the expression of TGF ? 1 intensively increased in hypertensive course, TGF ? 1 may be the important factor leaded to the renal tubulointerstitial fibrosis. Losartan has the effects on lowering blood pressure, reducing albuminuria, protecting renal function and delaying the renal tubulointerstitial fibrosis.