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Objective To explore the clinical significance of peripheral blood lymphocytesubsets detection in multiple myeloma (MM) patients with different immunophenotypes. Methods A total of 40 MM patients in People's Hospital of Xinjiang Uygur Autonomous Region from January 2016 to June 2016 were selected as the research objects, and 20 healthy examinations were treated as the control group. Marrow and blood samples were collected to detect the expressions of antigens and peripheral blood lymphocytesubsets by using flow cytometry. Besides, immunophenotype and the differences of lymphocytesubsets between the two groups were analyzed. Results The expression rates of CD138, CD38, CD56, CD19, CD20, CD45 were 100 %(40/40),100 %(40/40),75 %(30/40),0,20 %(8/40),0 in MM group and 100 %(20/20),100 %(20/20), 0,100 %(20/20), 100 %(20/20),75 %(15/20)in the control group.There were two kinds of immunophenotypes in MM group including CD138+CD38+CD56-CD19- (10 cases) and CD138+CD38+CD56+CD19- (30 cases). The absolute value of CD3+[(924±341)/ml vs. (1 474±270)/ml], CD4+[(410±163)/ml vs. (871±214)/ml], CD4+/CD8+[(1.0±0.3)/ml vs. (1.6±0.5)/ml], CD19+[(72±26)/ml vs. (277±89)/ml] was lower in MM group compared with the control group(P <0.05). There was no statistical difference in the absolute values of peripheral blood lymphocytesubsets for the patients with different immunophenotypes in MM patients (all P > 0.05). Conclusions Flow cytometry could help to type accurately the different immunophenotypes, distinguish the benign and malignant of plasmocytes and assist the diagnosis for the MM patients. Peripheral blood lymphocytesubsets analysis can be used to reflect the immunity of MM patients.
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Objective To review the phenylalanine(Phe) and thyrotropin(TSH) results of national external quality assessment(EQA)in neonatal screening laboratory for 7 years,evaluate qualitity control level and summarize the problems in the screening measurement.Methods The Phe and TSH values in dried blood spot specimens on filter paper distributed by national center for clinical laboratory were measured by fluorometrie method and time-resolved fluorescence immunoassay respectively.The Phe and TSH EQA results from 2010 to 2016 year were analyzed.Results Five specimens were distributed by EQA organizer each time,and 3 times each year.(1) In the 105 specimens of 21 batches,the bias ranges of Phe and TSH measurement were wide and their average values were 2.21% and 0.98% respectively.(2) Quantitative results analysis :Six quantitative results of Phe measurement and two quantitative results of TSH measurement were out of aunty control(bias ≥30% or ≤-30%),accounting for 5.71%(6/105)and 1.9%(2/105) respectively.(3)Qualitative results analysis:The Phe and TSH qualitative analysis of specimens conformed to the expected results(100%).The result of every batch was up to 80%.Conclusion It is useful to evaluate the measurement competence,find out the problems in the Phe and TSH measurement in neonatal screening laboratory and resolve them in time.Consequently,it is useful to improve the quality of measurement,reduce the error and ensure the accuracy of results.
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Objective To explore the proportion of different types of stones in patients with kidney stones in Xinjiang, and to analyze the relationship between stone composition and urine physicochemical properties and protein composition.Methods Through a case-control study, 355 patients with kidney stones who were hospitalized in the Xinjiang Uygur Autonomous Region People′s Hospital from March to November 2010 were enrolled in the same period, and non-urinary tract diseases were hospitalized and excluded from other diseases or complications of the 30 cases of renal impairment in the control group.The composition of the stones was analyzed.The main components of the stones were divided into oxalate group, carbonic acid group, uric acid group and phosphate group.The physicochemical properties and protein composition of 24 h urine were analyzed by ion selective electrode method, enzymatic and immunoturbidimetric assay.The difference between the two samples was analyzed by t test, and the differences between the two groups were analyzed by F-test and LSD-t test.Results Compared with the control group(30 cases), urine pH(5.33±0.32) was significantly lower in the oxalate group (244 cases), 24 h urinary calcium and 24 h uric acid[(7.68±0.35) mmol, (3.48±0.23)mmol (pH=5.874,P9.436, P8.442,P<0.05).Conclusion There may be a correlation between renal stone composition with urine metabolic changes.
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There are a variety of serological changes in the blood circulation of patients with lupus nephritis,including complement,autoantibodies,immunoglobulin and cytokines and so on.These substances not only involved in the development of the disease leads to renal pathological damage,but also can be used as a biomarker to reflect changes in disease.In this paper,the detection of serum markers in lupus nephritis diagnosis,determine the pathological classification,disease activity,evaluation of therapeutic effect and prognosis were reviewed,in order to provide scientific basis for the early diagnosis and treatment of lupus nephritis by monitoring the changes of serum indicators.
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Objective To detect serum level of long noncoding RNA ( lncRNA) BC200 in gastric cancer(GC) patients, and investigate its relationship with clinical features , and evaluate its diagnostic value for GC.Methods A case-control study was performed.From November 2014 to July 2015, serum levels of lncRNA BC200 were detected by real-time quantitative polymerase chain reaction in 124 patients with GC , 41 patients with atrophic gastritis and 59 normal controls who were hospitalized in Qilu Hospital of Shandong University.Meanwhile , serum carcinoembryonic antigen ( CEA ) and carbohydrate antigen 72-4 ( CA72-4 ) were detected by electrochemical luminescence immunoassay .Serum levels of lncRNA BC200, before and 3, 7, 10, 30, 100 days after radical operation in another 31 patients with GC were determined.The sensitivity and specificity of serum lncRNA BC200, CEA and CA72-4 were analyzed by using of the receiver operating characteristic ( ROC) curve.The comparison between two groups was performed with Mann-Whitney U test and the comparison among many groups was conducted with Kruskal-Wallis H test.Results Serum levels of lncRNA BC200 in GC patients with stage Ⅰ and Ⅱ[1.041(0.794,1.462)] and stage Ⅲ and Ⅳ[1.290 (0.978,1.794)]were significantly higher than those in patients with precancerous lesion [0.969(0.699, 1.219)]and normal controls[0.801(0.556,1.599)](H =54.68,P<0.000 1).Compared with pre-operation[1.120 (0.859,1.663)], the serum BC200 levels decreased significantly in 10 days [0.903 (0.724,1.182)](U=55.0,P<0.000 1), 30 days[0.759(0.671,1.037)](U=299.0,P=0.026 1), and 100 days[0.478(0.378,0.635)](U=41.0,P<0.000 1) after surgery.The area under the receiver operating characteristics curve ( AUC) of serum lncRNA BC200 was 0.865 for GC diagnosis, which was significantly higher than that of serum CA 72-4 ( AUC =0.699 ) or CEA ( AUC =0.807 ) .The AUC of combined detection of three tests was 0.934.Conclusion Serum lncRNA BC200 levels are significantly increased in GC patients , which may be used as a potential biomarker in GC diagnosis and monitoring .
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Objective To explore the clinical value of joint detection of urine light chains ,urine microalbumin and urine α1-mi-croglobulin in early diabetic kidney injury .Methods 218 cases type 2 diabetes mellitus(T2DM ) were selected as the observational group and 62 cases undergoing healthy physical examination as the control group .The rate scattering turbidimetry method was a-dopted to detect urinary κlight chains ,urinary λlight chains ,urinary micro-albumin(mALB) ,α1-microglobulin(α1-MG) in the two groups .Then the test results were performed the comparative analysis .Results The positive rate of urine κ light chains ,urine λlight chains ,mALB and α1-MG in the observational group were significantly higher than those in the control group ,the difference was statistically significant(P<0 .01);theκ/λvalue had no statistical difference between the observation group and control group (P>0 .05) .The receiver-operating characteristic curve analysis showed that the area under curve (AUC) of urineκlight chains ,u-rineλlight chains ,mALB and α1-MG ,and 95% CI were 0 .763 ,0 .541 ,0 .854 ,0 .807 and 0 .708 -0 .817 ,0 .464-0 .691 ,0 .811-0 .897 and 0 .758-0 .856 respectively ,AUC and 95% CI of joint detection of 4 urine indicators were 0 .919 and 0 .886-0 .951 ,the difference was statistically significant(P<0 .01) .Conclusion The combined detection of urine κ light chains ,urineλ light chains , mALB and α1-MG has an important clinical value for predicting early diabetic kidney injury .
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Objective To analyze the differences in urinary stone components between Uyghur and Han patients with urolithiasis in Xinjiang,and to explore the related factors contributing to the observed differences.Methods The data of urinary stone compo-nents in 317 adult patients with urolithiasis (152 cases of Uyghur nationality and 165 cases of Han nationality)admitted to Xinjiang Uygur Autonomous Region People′s Hospital from June 2009 to June 2011 were retrospectively analyzed.The urinary stone compo-nents were analyzed by the infrared spectroscopy.Results There were significant differences in thestone component proportions of calcium oxalate monohydrate (COD),COD+carbapatite (CA)and uric acid (UA)between Uyghur and Han patients(P <0.05). Conclusion The characteristics of the urinary stone comppnents have significant differences between Uyghur and Han patients with urolithiasis,which may be related with ethnicity and the different dietary habits.
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ObjectiveTo investigate the protein expressions of PTEN and p-Akt in Kaposi's sarcoma (KS) tissue and their significance in the development of KS.MethodsThe EnVision two-step method was used to detect the expressions of PTEN and p-Akt proteins in tissue specimens from the lesions of 17 patients with KS and from the normal skin of 17 normal human controls.ResultsPTEN and P-Akt were mainly expressed in the cytoplasm of cells.No significant difference was observed in the positivity rate of PTEN [76.5％(13/17)vs.88.2％(15/17),P＞ 0.10] between the KS and control specimens.p-Akt was expressed in all(100％) of the 17 KS specimens,but in none of the control specimens.ConclusionThe abnormal expression of PTEN protein may play a certain role in the development of KS.
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Objective To evaluate the value of pre-S1 Ag in combined detection of hepatitis B virus. Methods 288 patients' serums with chronicity type B hepatitis (CHB)were collected when they had been detected with HBV-DNA,HBVM or ALT/AST,and then ELISA assay were applied to detect pre-S1Ag. Results A total of 288 patients ,including 175 males and 113 females( their meso-age is 36.89 years old) were recruited into this study. 161 patients were pre-S1 Ag positive(68.4%), 197 patients' HBV DNA contents exceeded 5.0E + 2 copy/ml(55.9%) ,71patients were H BeAg positive (24.6%), 186 patients were HBeAg negative (64.58%). The rate of 64 patients' ALT and AST exceeded 0. 84, and their' ALT exceeded 40 u/ml ( 22. 2% ). 60 patients' AST exceeded 50 u/ml (20. 8% ). These patients were divided into pre-S1 Ag positive group and pre-S1 Ag group. In pre-S1 Ag positive group, 124 patients' HBV DNA contents exceeded 5.0E + 2 copy/ml( 62.9% ), 118 patients were HBeAg negative (59.8%). When HBV DNA content was less than 5.0E+2 copy/ml,pre-S1 Ag positive rate was 35.2% and pre-S1 Ag negative rate was 63.6%. when HBV DNA content was less than 5.0E + 3 copy/ml, pre-S1 Ag positive rate was 56.9% and pre-S1 Ag negative rate was 81.7%. When HBV DNA content exceeded 5.0E + 5 copy/ml but less than 5.0E + 8 copy/ml ,pre-S1 Ag positive rate was 33% and pre-S1 Ag negative rate was 14.3% ,so two groups had significant difference. Conclusion Pre-S1 Ag positive and HBeAg negative in the CHB patients serums signify virus replication,and it can help us decide prognosis.
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Objective To study the effect of flee radical on kidney repedusion injury caused by infra-renal abdominal aorta occlusion in rat model and its possible mechanism. Methods Forty-two healthy Wis-ter rats were randondy divided into 6 groups as following ( n = 7) : the control group( sham group) ; simply isehemia 5 h without reperfusion( group I) ; 2 hours reperfusinn following ischemia 5 h ( I/R 2 h), 4 honrs reperfusion following isebemia 5 h ( I/R 4 h), 8 hours reperfusinn following ischemia 5 h( I/R 8 h) and 12 hours reperfusion following ischemia 5 h ( I/R 12 h). In each group the rats were killed to obtain samples of blood and kidney at the specified time points. The contents of BUN, Cr, MDA, SOD in blood and in renal homogenate were measured in each group. We observed the morphological changes of kidney and muscles of lower limb by light microscope. Results BUN level of serum in model group I, I/R 2 h, I/R 4 h, I/R 12 h were higher obvionsly than those of control group, which were maximal in I/R 4 h, then decreased. MDA level of plasma in model group I, I/R 2 h, I/R 4 h, I/R 8 h, I/R 12 h groups were higher obvionsly than those of control group, which were maximal in I/R4 h group, then decreased. SOD level of plasma in model I/R 4 h, I/R 8 h groups were lower obviously than those of control group; SOD level of renal homogenate in model group I, I/R 2 h, I/R 8 h, I/R 12 h groups were higher obviously than those of group I/R 4 h, which were minimal in I/R 4 h group, then increased. By light microscope: The injury degree of kidney and muscles of lower limb in ischemia group was slight, the injury degree of repedusion group was severer than ischemia group. Conclusion The kidney repedusion injury caused by infrarenal abdominal aorta occlusion in rat model might be concerned with the increase of lipid peroxidatian damage after ischemia-repedusion in-jury of lower limbs.