ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of different proportions of cryoprotectant to seminal plasma on the motility of post-thaw human sperm.</p><p><b>METHODS</b>Different proportions of cryoprotectant to seminal plasma (1:1 and 1:3) were used for freezing sperm, and the forward movement and total motility rates of the frozen-thawed sperm were compared.</p><p><b>RESULTS</b>The forward movement and total motility rates were (58.60 +/- 5.57)% and (66.17 +/- 5.24)% before cryopreservation. The 1:1 proportion achieved post-thaw forward movement and total motility rates of (40.53 +/- 8.97)% and (51.23 +/- 9. 30)%, while the 1:3 (44.7 +/- 8.67)% and (51.50 +/- 7.40)%, respectively. Significantly decreased sperm motility was observed after cryopreservation (P < 0.05). Statistically significant differences were found in the forward movement but not in the total motility of the frozen-thawed sperm between the two proportions.</p><p><b>CONCLUSION</b>Cryopreservation causes obvious damage to human sperm. Higher proportion of cryoprotectant to seminal plasma (1:3) can improve the forward movement of post-thaw sperm as compared with the lower one (1:1).</p>
Subject(s)
Adult , Humans , Male , Cryoprotective Agents , Pharmacology , Freezing , Semen , Sperm MotilityABSTRACT
<p><b>OBJECTIVE</b>To compare the effects of the cryoprotectant containing glucose and that containing sucrose on the motility of post-thaw human sperm.</p><p><b>METHODS</b>The cryoprotectant containing glucose and that containing sucrose were applied to 50 semen samples and the motility of the post-thaw human sperm was compared before and after cryopreservation and between the study groups.</p><p><b>RESULTS</b>The forward motility and total motility of the sperm were (58.4 +/- 5.7)% and (63.4 +/- 6.1)% before cryopreservation, (43.8 +/- 7.6)% and (48.4 +/- 7.6)% after thawing with the cryoprotectant containing glucose, and(42.6 +/- 8.9)% and (48.0 +/- 8.5)% after thawing with the cryoprotectant containing sucrose. Decreased sperm motility was observed after cryopreservation, with statistic significance (P < 0.01). There was no significant difference in the forward and total motility of the post-thaw sperm between the two cryoprotectants.</p><p><b>CONCLUSION</b>Cryopreservation inflicts obvious damage on sperm. Sucrose is a feasible sperm cryoprotectant.</p>