Molecular mechanism of exercise intervention in diabetic cardiomyopathy in rats / 中国病理生理杂志

AIM: To investigate the effect of streptozotocin-induced diabetes on the expression of NADPH oxidase, and to determine the effect of exercise on the expression of NADPH oxidase.METHODS: One weeks after successful modeling, the effect of diabetes mellitus on the expression of NADPH oxidase subunits in the cardiac tissue was determined.The effect of exercise for 8 weeks on the protein expression of NADPH oxidase subunits and the effect of diabetes on the protein expression of collagen in the heart were observed, and whether 8 weeks of exercise affected the expression of collagen were also measured.RESULTS: Diabetes mellitus resulted in the increased expression of NADPH oxidase subunits p47phox and gp91phox in the cardiac ventricle, and exercise for 8 weeks inhibited the increase in the expression of NADPH oxidase subunits p47phox and gp91phox.Diabetes mellitus significantly increased the expression of p47phox in the atrial muscle, while exercise inhibited this increase.Diabetes mellitus significantly increased the levels of cardiac collagen III, while exercise reduced the increase in collagen protein.CONCLUSION: Reduction of diabetic rat heart p47phox and gp91phox expression by effective exercise therapy may be an important mechanism of improving the cardiac matrix by inhibiting myocardial oxidative stress and decreasing collagen expression, thus preventing diabetic cardiomyopathy.

Strategies of tumor-targeted gene therapy / 中国癌症杂志

Targeting of tumor cells is crucial for gene therapy of malignant diseases.This can be achieved by tumor targeted gene transfer or tumor specific gene expression, as well as secretion of tumor targeted therapeutic molecules by autologous normal cells.Tumor targeted gene transfer is mediated by the recognition of a class of tumor specific antigens or receptors by corresponding vector fused antibodies or ligands, while therapeutic genes can be selectively expressed in tumor cells under the control of tumor or tissue specific promoters or enhancers, as well as the induction of certain physical, chemical or physiological factors.

Improved PCR-based subtractive hybridization, a new strategy on cloning differential expression genes in apoptotic MCF-7 cells / 细胞与分子免疫学杂志

Aim To clone apoptosis-related genes from human MCF-7 breast cancer cells and to analyze the character of the method used in the process. Methods A poptotic cell model of MCF-7 cells was established with the apoptotic tumor cells induced by the all-trans-retinoic acid. The apoptotic gene was cloned from the model by improved PCR-based subtractive hybridization. Results 5 clones were identified to be related to apoptosis by reverse dot blot, 4 of them were known genes, and 3 were related to apoptosis. A novel gene， named apmcf-1, coded for 47 amino acid was identified. This gene was accepted by Genbank, the accession number was AF141882. Conclusion This improved PCR-based subtractive hybridization may be an efficient way in cloning differential expression gene.

The Expression of Anti-bcl-2 Ribozyme Gene in HL-60 Cells / 中国肿瘤生物治疗杂志

A hammerhead RZ DNA was designed and synthesized, which can specifically cleave the bcl-2 mRNA. After demonstration of right sequences by sequencing and cleavage activity of RZ by in vitro cleaving experiment, The RZ DNA was recombinated into the pDOR - neo vector to form the recombinant pDOR - RZ. Using lipofectin - mediated DNA transfectionpDOR-RZ was successfully introduced into HL - 60 cells. The RZ expression was observed by Southern, RNA dot blot hybridization and flow cytometry (FCM) . The results demonstrated that (a) the RZ was expressed in 72 hours after transfection; (b) the synthesis of Bel - 2 protein was inhibited by the expression of RZ; (c) apoptotic peak appeared in FCM.