Effect of danusertib on cell cycle, apoptosis and autophagy of hepatocellular carcinoma HepG2 cells / 南方医科大学学报

OBJECTIVE@#To investigate the effect of danusertib (Danu), an inhibitor of Aurora kinase, on the proliferation, cell cycle, apoptosis, and autophagy of hepatocellular carcinoma HepG2 cells and explore the underlying mechanisms.@*METHODS@#MTT assay was used to examine the effect of Danu on the viability of HepG2 cells to determine the IC50 of Danu. The effect of Danu on cell cycle distribution, apoptosis and autophagy were determined using flow cytometry. Western blotting was used to detect the expressions of the proteins related to cell cycle, apoptosis and autophagy. Chloroquine was used to suppress Danuinduced autophagy to test the apoptosis-inducing effect of Danu.@*RESULTS@#Danu significantly inhibited the proliferation of HepG2 cells with IC of 39.4 μmol and 14.4 μmol at 24 h and 48 h, respectively. Danu caused cell cycle arrest in G/M phase in HepG2 cells and led to polyploidy accumulation via up-regulating the expressions of p53 and p21 and down-regulating the expressions of cyclin B1 and DC2. Danu also caused apoptosis of HepG2 cells through up-regulating the expressions of Bax, Puma, cleaved caspase-3, cleaved caspase-9, cleaved PARP and cytochrome C and down-regulating the expressions of Bcl-xl and Bcl-2. Danu induced autophagy via activating AMPK signaling and inhibiting PI3K/PTEN/AKT/mTOR axis, and inhibition of Danu-induced autophagy with chloroquine enhanced the pro-apoptotic effect of Danu.@*CONCLUSIONS@#Danu inhibits cell proliferation and induces cell cycle arrest in G/M phase, apoptosis and cytoprotective autophagy in HepG2 cells.

Research on H. pylori eradication treatment with iron deficiency anemia / 中国医学装备

Objective: To study Helicobacter pylori (HP) infection and iron deficiency anemia (IDA), to explore the HP radical IDA treatment effect. Methods:To select Hospital 584 healthy volunteers, analysis of the composition of IDA and HP infection, diagnosed 61 cases of IDA merger of HP infection were randomly divided into observation group and control group, observed 31 patients given iron joint anti-HP treatment, the control group of 30 patients treated with iron therapy, the therapeutic effect of the two groups were compared. Results:The group of 584 volunteers, IDA combined HP infection in 61 patients;the IDA patients HP infection rate was 55.96%, significantly higher than the non-IDA crowd the 20.21%;HP infection by IDA prevalence rate of 38.85%, significantly higher than the HPnegative 11.24%;incidence of HP infection and IDA have significant correlation, x2=15.3403, P<0.001. Four weeks after the observation group anti-HP therapy disable HP cure in 29 cases, the cure rate of 93.55%. After treatment IDA indicators have improved to some extent, the observation group after treatment, 1 month, 3 months increased significantly compared with those before treatment (t=3.974, 4.802, 5.221, 6.308;P<0.01);only one indicator of a treatment in the control group after treatment before a significant increase (t=5.383, P<0.01), 3 months after treatment, a significant increase in the indicators than before treatment (t=4.446, 3.741, 8.221;P<0.01), but significantly lower than the same period in the observation group (t=4.201, 6.664, 3.847;P<0.01). Conclusion:HP infection and IDA is closely related to the cure HP infection is of great significance to improve the IDA treatment of effect.