ABSTRACT
Background: Epidermal Growth Factor Receptor (EGFR) is a transmembrane cell-surface glycoprotein with intrinsic tyrosine kinase activity. EGFR has been shown to stimulate cell proliferation and to enhance the migration and invasiveness of breast cancer. EGFR is expressed in epidermal cell lines and have been implicated in the pathogenesis of many different types of cancer. Objective: To evaluate the level of EGFR transcript in breast cancer and normal tissues; comparison the EGFR transcript level at different development stages and cancer cell types. Subject and methods: Total RNA from 62 tissue samples including 47 breast cancer and 15 normal tissues were extracted; cDNA synthesis by reverse transcript polymerase chain reaction, EGFR transcript level were determined using semi-quantitative RT-PCR. Result and conclusions: EGFR transcript level was highly expressed in breast cancer tissues compared to the normal tissues. Especially, its expression was related to the different status and cancer cell types of breast cancer. There was a difference of EGFR transcript level between histological pathology\u2019s forms of breast cancer in the same stage.
Subject(s)
Breast Neoplasms , ErbB ReceptorsABSTRACT
Background: Human Heparansulphate interacting protein (hHip) has been shown to participate in biological processes of cells. Several studies indicated that hHip transcript is up regulated in several of cancer tissues including those of thyroid, colon, breast and prostate. Antibody against hHIP is necessary for methods to evaluate protein level of HIP in cancer tissues. Objectives:The aims of study was to induce anti hHIP antibody in rabbit and purify and conserve purified anti hHIP antibody. Subjects and method: The study included 9 adult and healthy rabbits with the weight 2 - 2.5kg. Immunization hHIP peptide-KLH in rabbit. Purify anti hHIP antibody using affinity chromatography. Results: The results shown synthesize hHIP peptide and conjugate it with carrier protein. Sensitive rabbit better meet with hHIP-KLH antibody. The Ig concentration obtained in sensitive rabbit was rather high and equal. Immunization hHIP-KLH successfully in rabbit. Obtainment valuable amount of anti hHIP antibody. Conclusion: Successfully induce and purify anti hHIP antibody from rabbit. Establish a standard protocol for polyclonal antibody against small peptide in rabbit.\r\n', u'\r\n', u'
Subject(s)
Rabbits , Carrier Proteins , Dosage , ChemistryABSTRACT
385 Kinh and Muong vasectomized men and 40 men (control) were subjective of the study. All are healthy, ages of 30-60, all had at least a child. The blood samples were taken at 6 weeks, 3-6 months, 7-12 months and 6 years after operation. The Franklin - Duker agglutination test and passive immunofluorescence test for detecting sperm antibodies were used. The results were as follows: in 40 control, there was only one, who had agglutinating sperm antibodies, i.e 2.5%; 39.5% incidence of sperm antibodies were found in vasectomized men one year after operation. In the time of 6 weeks, that was 9%, less three months was 12.1%, 3 to 6 months was 40.3%, 7 to 12 months was 48.8%; 25.3% vasectomized men exhibited sperm antibodies 1 to 6 years after operation; the sperm antibodies incidence of Kinh men was lower than Muong men, that were 38.4% and 44.4% respectively; the antibodies detected by immunofluorescence test were IgG binding in the acrosome or neck and tail of spermatozoa