1.
Braz. j. med. biol. res
;
33(8): 897-903, Aug. 2000. ilus, tab
Article
in English
| LILACS
| ID: lil-265846
ABSTRACT
We describe here the isolation and characterization of a major albumin from the seeds of Cereus jamacaru (Cactaceae), to which we gave the trivial name of cactin. This protein has a molecular mass of 11.3 kDa and is formed by a light chain (3.67 kDa) and a heavy chain (7.63 kDa). This protein was isolated using a combination of gel filtration chromatography and reverse-phase HPLC. The amino acid composition of cactin was determined and found to resemble that of the 2S seed reserve protein from the Brazil nut, a protein remarkable for its high methionine content. The usefulness of cactin as a molecular marker in the taxonomy of the Cactaceae is discussed
Subject(s)
Albumins/analysis , Methionine/analysis , Plant Proteins/analysis , Seeds/chemistry , Chromatography, High Pressure Liquid , Electrophoresis, Polyacrylamide Gel , Methionine/chemistry , Plant Proteins/chemistry
2.
J. pediatr. (Rio J.)
;
52(1/2): 53-4, 1982.
Article
in Portuguese
| LILACS
| ID: lil-8932