ABSTRACT
This study is to examine the relationship between TGF-b1 expression and CTGF expression, and to evaluate the effect of Sp1 blockade on the expression of TGF-b1, CTGF and extracellular genes, clones of fibroblasts stably transfected with Sp1 decoy ODN. R-Sp1 decoy ODN was highly resistant to degradation by nucleases or serum, compared to the linear or phosphorothioated-Sp1 decoy ODN. Skin wounds were created on the back of 36 anesthetized rats. They were divided into four groups-the rats with normal skin, with wounded skin without decoy, with wounded skin injected with R-Sp1 decoy, and with wounded skin injected with mismatched R-Sp1 decoy, respectively. Skins were collected at 3rd, 5th, 7th, 14th day after wounding. Cellular RNA was extracted by RT-PCR analysis. TGF-beta1 and CTGF were deeply related with skin fibrosis during scar formation and it appeared that TGF-beta1 may cause the induction of CTGF expression. R-Sp1 decoy ODN inhibited TGF-beta1 and CTGF expression both in cultured fibroblasts and in the skin of rats. These results indicate that targeting Sp1 with R-type decoy efficiently blocks extracellular matrix gene expression, and suggest an important new therapeutic approach to control the scarring in normal wound healing and fibrotic disorders.
Subject(s)
Animals , Rats , Cicatrix , Clone Cells , Extracellular Matrix , Fibroblasts , Fibrosis , Gene Expression , RNA , Skin , Transforming Growth Factor beta1 , Wound Healing , Wounds and InjuriesABSTRACT
A 36-year-old female who initially presented with a small erythematous and swollen abscess on her left anterior tibial area was found out to have a cutaneous Mycobacterium abscessus infection. She was first treated with incision and drainage, dressing, and antibiotics. The lesion began to be aggravated and dispersed. Neither aerobic nor anaerobic bacteria was grown on blood agar plate. After a few weeks, Mycobacterium grew on Ogawa media after 6 days, and was identified as M. abscessus by PCR-restriction fragment length polymorphism. She was then treated with clarithromycin, levofloxacin, and amikacin, and the skin lesion was resolved without further recurrence.
Subject(s)
Adult , Female , Humans , Abscess , Agar , Amikacin , Anti-Bacterial Agents , Bacteria, Anaerobic , Bandages , Clarithromycin , Drainage , Levofloxacin , Mycobacterium , Recurrence , Skin , Soft Tissue InfectionsABSTRACT
Congenital arteriovenous malformation is one of developmental anomaly of vascular system. Since the lesion consist of abundant vascular component, feeding arteries and draining vessels, most of the lesions show poor demarcation and even show invasion to the adjacent tissues. Because of this characteristics, management of this malformation is troublesome. Selective arterial embolization and excision of the lesion is one of the recommended management. We experienced congenital arteriovenous malformation at the buttock of 18 years old male patient. He had been performed multiple feeding arterial embolization using Histoacryl with lipiodol 2 years ago because of repeated bleeding. But that buttock bleeding recurred 2 years later. After confirmation of the lesion by MRI and angiogram, wide excision and ligation of feeding artery was done. The wound was repaired by posterior thigh flap.
Subject(s)
Adolescent , Humans , Male , Arteries , Arteriovenous Malformations , Buttocks , Enbucrilate , Ethiodized Oil , Hemorrhage , Ligation , Magnetic Resonance Imaging , Thigh , Wounds and InjuriesABSTRACT
The management of orbital dystopia has ranged from camouflage procedure for minor deformity to vertical translocation of the displaced orbit for severe deformity. The purpose of this study is to provide a guideline for the selection of an appropriate surgical treatment according to the degree of the dystopia. Since 1989, 18 patients with orbital dystopia have been treated for all cosmetic reasons. There were 13 females and 5 males aged from 8 to 46 years (mean 18.8 years). Underlying pathology in our cases included traumatic (orbital floor and wall fractures and irradiation)(44%); congenital (cleidocranial dysostosis, dermoid cyst, facial clefts and idiopathic causes)(40%); and fibrous dysplasia(16%). In the coronal plane, thr orbital displacement ranged from 2 to 7 mm(mean 3.9 mm). Patients were grouped into severe(n=8), moderate(n=6), and mild(n=4) degree of dystopia. Orbital repositioning, such as vertical repositioning of the functional orbit, bone shaving, autogenous bone graft and alloplastic material implantation were performed in severe cases(>4 mm). Various eye leveling technique, such as autogenous bone onlay graft, alloplastic material implantation, medial and superior rotation of zygoma, and medial and lateral canthoplasties were performed in moderate (3 mm) and mild (< 2 mm) cases. The postoperative surgical result was assessed by ordinary scale method cephalometric analysis. There was no visual loss, infection, epilepsy, or asethetic complication. The preoperative findings of orbital dystopia were corrected or significantly improved following surgery in all patients after an average follow-up period of 59.2 months. The result of this study indicate that our method of surgical correction of vertical orbital dystopia according to the degree of the dystopia is resonably safe, rewarding and worthwhile to the patients.