ABSTRACT
Background and Aim: the most common primary brain tumors of the central nervous system are gliomas. Among a number of different biomolecular events, a strong relation between oxidative stress pathways and the development of this cancer has been proved. Oxidative stress [OS] is the consequence of an imbalance between pro-oxidants and antioxidants towards pro-oxidants. The pro-oxidants cause lipid peroxidation of cell membranes, protein, DNA oxidation and changes in brain cell growth. In this study, the pro-oxidant-antioxidant balance [PAB] was determined in patients with gliomas
Methods and Materials/Patients: sera of 49 patients with high grade glioma [grade IV WHO] which is called glioblastoma multiform [GBM] and 49 healthy subjects were collected and PAB test was measured
Results: a significant increase of PAB value was observed in patients with GBM [158.10+/-85.71 HK unit] in comparison to control group [74.54+/-33.54 HK]
Conclusion: the PAB assay showed the oxidative stress in glioblastoma. In further research, this easy elucidation of oxidative stress in these patients can be applied to evaluate the effectiveness of antioxidant therapies in patients with GBM
ABSTRACT
The cultured mesenchymal stem cells [MSC] have been used in many clinical trials; however, there are still some concerns about the cultural conditions. One concern is related to the use of FBS as a widely used xenogeneic supplement in the culture system. Human platelet-rich plasma [hPRP] is a candidate replacement for FBS. In this study, the effect of hPRP on MSC proliferation and osteogenic differentiation has been evaluated. Human adipose-derived stem cells [hADSC] were expanded. Cells from the third passage were characterized by flow cytometric analysis and used for in vitro experiments. Resazurin and alizarin red stains were used for cell proliferation and osteogenic differentiation assays, respectively. Treatment with hPRP resulted in a statistically significant increase in cell proliferation compare to the negative control group [P<0.001]. Cell proliferation in the 15% hPRP group was also significantly higher than that in the 10% hPRP group [P<0.05]. Additionally, it caused less osteogenic differentiation of the hADSC compared to the FBS [P<0.001], but in comparison to negative control, it caused acceptable mineralization [P<0.001]. These findings indicate that hPRP not only improves the proliferation but also it can be a suitable substitution in osteogenic differentiation for clinical purposes. However, the clinical application value of hPRP still needs more investigation
ABSTRACT
Congenital chyloperitoneum is an uncommon clinical condition. A few cases of congenital chyloperitoneum in children have been described who were treated in a variety of methods. Congenital chyloperito was diagnosed in a 5-day-old baby boy with a significant abdominal distension. Due to the failed conservative managements by medium-chain triglycerides [MCT] enriched milk and partial parenteral nutrition [PPN], the authors tried platelet rich fibrin glue [PRFG] as an alternative choice which was applied through an already inserted intra-abdominal catheter. PRFG successfully stopped the lymph leakage from all over the small intestinal mesentery; thereby PRFG may be considered as an effective alternative treatment before surgical intervention. Applying PRFG is an easy, safe, and effective alternative option that may be used to close the chylous ascites lymph leakage in children if conservative management with PPN fails
Subject(s)
Humans , Male , Chylous Ascites/congenital , Fibrin Tissue Adhesive/therapeutic use , Blood PlateletsABSTRACT
Comparison of prooxidant-antioxidant balance [PAB] assay with crocin assay. Twenty eight serum samples were chosen, PAB and the total antioxidant capacity were measured by PAB assay and crocin, respectively, and the correlation of both assays, along with their correlation with other clinical and biochemical parameters, were determined. A significant negative correlation was established between PAB assay and crocin assay. Also a significant negative correlation was established between PAB and uric acid and creatinine. The results showed that by increasing the total antioxidant capacity, which is showed by crocin, the PAB shifts in favor of antioxidants, which is showed by PAB assay. Now, it could be considered that the PAB, along with other risk factors, might help in the prediction of the risk for cardiovascular events; and further research could clarify whether by application of PAB assay and appropriate interventions for correcting oxidative stress, progression of the cardiovascular disease could be reduced