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1.
Braz. j. microbiol ; 48(2): 366-372, April.-June 2017. tab, graf
Article in English | LILACS | ID: biblio-839381

ABSTRACT

Abstract Malignant Catarrhal Fever (MCF) was investigated in the central nervous system of cattle with neurological syndrome. Two-hundred-ninety samples were analyzed by histology, and molecular methods to detect ovine herpesvirus type 2 (OvHV-2) were optimized and validated. The qualitative polymerase chain reaction (qualitative PCR) analytical sensitivity was 101 DNA copies/µL and found 4.8% (14/290) positive for OvHV-2. The quantitative polymerase chain reaction (qPCR) analytical sensitivity was 100 DNA copy/µL and 5.9% (17/290) positivity, with 47.1% (8/17) of the positive samples presenting histological evidence of non-purulent meningo-encephalitis. The qualitative PCR products (422 bp of the ORF75 region) were sequenced and submitted to phylogenetic analysis. Identity matrices showed 100% similarity in OvHV-2 samples obtained in this study and those recovered from GenBank, corroborating other studies.


Subject(s)
Animals , Phylogeny , Molecular Diagnostic Techniques/methods , Herpesviridae/isolation & purification , Malignant Catarrh/diagnosis , Malignant Catarrh/pathology , Brazil , Cattle , Cluster Analysis , Polymerase Chain Reaction/methods , Sensitivity and Specificity , Sequence Homology , Sequence Analysis, DNA , Genotype , Herpesviridae/classification , Herpesviridae/genetics , Histocytochemistry , Microscopy
3.
J Indian Med Assoc ; 1971 Nov; 57(9): 321-3
Article in English | IMSEAR | ID: sea-103683
5.
7.
J Indian Med Assoc ; 1965 May; 44(): 520-4
Article in English | IMSEAR | ID: sea-100135

Subject(s)
Cholera , Classification , India , Vibrio
8.
11.
J Indian Med Assoc ; 1957 Jun; 28(12): 514-9
Article in English | IMSEAR | ID: sea-103592
12.
J Indian Med Assoc ; 1953 Nov; 23(2): 60-8
Article in English | IMSEAR | ID: sea-105628
13.
J Indian Med Assoc ; 1952 Mar; 21(6): 242-5
Article in English | IMSEAR | ID: sea-97486
14.
J Indian Med Assoc ; 1951 Dec; 21(3): 95-8
Article in English | IMSEAR | ID: sea-95692

Subject(s)
Malaria/blood
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