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Objective:To study the effects of beta tricalcium phosphate(β-TCP)/collagen scaffold loaded with human bone morphogenetic protein 2 (hBMP2) plasmid on the osteogenesis ability of MC3T3-E1 cells.Methods:hBMP2 DNA plasmid-modified β-TCP/collagen scaffold and the naked plasmid(control) were constructed.MC3T3-E1 cells were respectively in vitro cultured onto the β-TCP/collagen scaffold with hBMP2(Z) and with control plasmid(Z0),on peace dish with the saffold and hBMP2(M) and with the control plasmid(M0).The surface morphology of the samples was observed by SEM.Osteogenesis of the cells was examined by alkaline phosphatase activity(ALP) test,real-time fluorescent quantitative PCR for the detection of Runx2,OCN,ALP and OPN mRNA expression.Data were statistically analyzed.Results:The composite sample surface of plasmid DNA containing hBMP2 modified β-TCP/collagen was porous;group Z and M showed highter ALP activity and higher mRNA expression of Runx2,OCN,ALP and OPN than group Z0 and M0;so did group Z than group M.Conclusion:Porous β-TCP/collagen scaffold loaded with BMP2 DNA is potential for osteoinduction.
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<p><b>OBJECTIVE</b>To investigate interleukin-33 (IL-33) in the arterial vascular endothelium of rabbits infected with Porphyromonas gingivalis (P. gingivalis), and to explore the relationship between P. gingivalis and atherosclerosis.</p><p><b>METHODS</b>A total of 24 rabbits were randomly divided into control and experimental groups. The experimental group received intravenous injection of P. gingivalis once a week for 12 weeks to establish a coronary atherosclerosis model. The rabbits in the control group were injected with equal volume of physiological saline. All the rabbits were killed after 13 weeks. The IL-33 expression levels in the arterial vascular endothelium of the rabbits were detected through immunohistochemistry, reverse transcription polymerase chain reaction, and Western blot analysis. The effects of P. gingivalis on the IL-33 expression in the arterial vascular endothelium of the rabbits were analyzed.</p><p><b>RESULTS</b>The relative expression levels of IL-33 mRNA in the vascular endothelium cells were 58.244±2.407, and the relative expression levels of IL-33 protein were 1.863±0.171 in the experimental group. The relative expression levels of IL-33 mRNA were 3.143±0.805, and the relative expression levels of IL-33 protein were 0.537± 0.028 in the control group. The expression levels of IL-33 mRNA and protein of vascular endothelium cells in the experimental group were significantly higher than those of the control group (P<0.01).</p><p><b>CONCLUSIONS</b>P. gingivalis infection promotes IL-33 expression levels in vascular endothelial cells and may regulate the occurrence and development of atherosclerosis. .</p>
Subject(s)
Animals , Humans , Rabbits , Atherosclerosis , Endothelial Cells , Endothelium , Endothelium, Vascular , Interleukin-33 , Interleukins , Porphyromonas gingivalisABSTRACT
10.3969/j.issn.2095-4344.2013.25.002
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Objective: To observe the biocompatibility of acellular nerve scaffold (ANS) via three sterilization methods, to provide experimental data for tissue engineering industrialization. Methods: Pig sciatic nerves were cut and treated using the NaOH maceration method. ANSs were sterilized by ethylene oxide, ~(60)Co-irradiation and peracetic acid. Evaluated the biocompatibility by MTT, cellular compatibility test, collagenase susceptibility test in vitro and local implantation test. Results: ANS retained the integrity of structure and major components of the basement membrane. The result of MTT test showed that the ANSs via different sterilization methods had statistical differences. There were no overall significant differences in Collagenase susceptibility test. Scanning electron microscope results showed the skin fibroblasts could attach, proliferate and grow well on the surface and holes of ANS with sterilization of PAA and Co~(60),a small quantity of cells adhered on ANS with sterilization of ETO. Tests for local effects after implantation show that different sterilization methods don't effect the ability of ANS to resist the enzyme degradation. In ETO group, rats showed an acute inflammatory response followed by chronic inflammation. In PAA and ~(60)Co group rats showed an acute inflammatory response that diminished such that the graft ultimately became indistinguishable from native tissue, observations that were consistent with graft acceptance. Conclusion: Peracetic acid sterilization offers a convenient alternative protocol for ANS processing. ANS sterilized with PAA shows good compatibility and biologic safety. It is an ideal sterilization method for ANS.
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BACKGROUND: Practice has proved that organic material and inorganic materials used alone are not ideal scaffold materials. Polylactic acid (PLA) possessing excellent biocompatibility, degradability and absorbability, PLAs composites will be one of the most important biocomposite in the 21~(st) century. OBJECTIVE: To observe the effects of PLA-chitosan fiber (CF)/hydrexyapatite-calcium silicate (HA-CS) on adhesion, proliferation and differentiation of osteoblasts. METHODS: The rat osteoblasts were obtained from the cranium of newborn Wistar rats within 24 hours, and primarily cultured using modified collagenase digestion. The cells were generated and their biological characteristic was examined by inverted phase-contrast microscope, hematoxylin-eosin staining, alkaline phosphatase (ALP) staining and mineralized nodules staining. Then the cells at passage 3 were co-cultured with PLA-CF, PLA-CF/CS and PLA-CF/HA-CS in vitro. At 3, 6 and 9 days of the culture, cell morphology was observed by inverted phase contrast microscopy. In addition, MTT assay and ALP activity test were used to observe the effects of three kinds of materials on cell differentiation and proliferation. RESULTS AND CONCLUSION: Osteoblasts attached on all three scaffold materials can adhere, grow, differentiate and proliferate. The effect of three materials on cell activity was PLA-CF/HA-CS>PLA-CF/CS>PLA-CF. The composite scaffold PLA-CF/HA-CS has a good compatibility, indicating that the material has a great potential for application in bone tissue engineering.