ABSTRACT
Objective@#To detect differentially expressed microRNAs in chronic hepatitis B (CHB) before being treated with pegylated interferon (PegIFN) and the relationship between their target genes and HBsAg loss.@*Methods@#Pretreatment differentially expressed microRNAs between different response groups were screened using high throughput microarrays and validated by quantitative reverse-transcription polymerase chain reaction (RT-qPCR). Bioinformatics analysis was performed to determine their target genes potential mechanistic roles.@*Results@#A total of 417 microRNA were differentially expressed between different response groups, among which 342 were up-regulated and 75 were down-regulated. miR-3960, miR-126-3p, miR-23 a-3p and miR-335-5p were verified to be down-regulated by RT-qPCR result in HBsAg loss group. Bioinformatic analysis result show that the relevant pathways of microRNAs include AMPK signal pathway, NOD-like signal pathway, NF-kappa B signal pathway and mTOR signal pathway.@*Conclusions@#HBsAg loss is probably achieved as the result of genes expression regulated in association with immune response, further enhance the immune response of HBV elimination and acquire HBsAg loss.
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Objective To investigate the influence on patients' coagulation of different anticoagulants during the treatment of severe hepatitis with ALSS (artificial liver support system).Methods According to different means of medication,20 severe hepatitis patients were divided into two groups randomly:heparin group,LMWH (low-molecular-weight heparin) group,and the two groups were provided with ALSS.The coagulation changes were evaluated before and after the treatment in two groups individually.Results There was no significant difference in hemoglobin levels before and after treatment in two groups.Prothrombin time (PT) and activated partial thromboplastin time (APTT) prolonged significantly before treatment,but returned to normal after treatment in heparin group;the two values were significantly lowered after treatment in LMWH group.There were increased fibrinogen level,thrombin time (TT) and decreased factor Ⅷ levels in both heparin and LMWH group.Many complications,such as blood pressure lowering,might occur in heparin group,but not in LMWH group.Conclusion It is effective and safe to use LMWH for severe hepatitis patients when treating with ALSS.
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Objective To investigate the influence of vitamin E on type Ⅰ collagen, messenger RNA expression of transforming growth factor ? 1 (TGF? 1) and tissue inhibitor of matrix metalloproteinases-2 (TIMP 2) in hepatic stellate cells (HSC). Methods HSC s were cultured in vitro, the effect of vitamin E on type Ⅰ collagen was observed by mean of immunohistochemistry; mRNA expression of TGF? 1 and TIMP 2 were investigated with in situ hybridization. Results In experiment group, vitamin E markedly inhibited expression of type Ⅰ collagen of HSC as compared with control group, but could not suppress mRNA expression of TGF? 1 and TIMP 2.Conclusions Anti-fibrotic effect of Vitamin E may be implemented through inhibiting the expression of type Ⅰ collagen.