ABSTRACT
Objective To understand the clinical significance of detection of tumor necrosis factor alpha (TNF -α),neuron specific enolization enzyme (NSE)and S100B levels in children with hand -foot -mouth disease (HFMD)complicated with encephalitis,and to provide research data for children with HFMD complicated with encephalitis.Methods 157 children with HFMD were divided into 79 cases of HFMD complicated encephalitis group and 78 cases of HFMD group according to whether there was encephalitis complications.Another 80 healthy children were chosen as control group.TNF -α,NSE and S100B protein in blood and cerebrospinal fluid of the three groups were detected by enzyme -linked immunosorbent assay.Results TNF -αlevels in blood and cerebrospinal fluid of HFMD complicated encephalitis group,HFMD group were significantly higher than those of the control group (t =8.315,6.443,4.132,6.443,all P <0.05);TNF -αlevels in blood and cerebrospinal fluid of HFMD complicated encephalitis group were significantly higher than those of HFMD group (t =4.378,3.220,all P <0.05).NSE in blood and cerebrospinal fluid of HFMD complicated encephalitis group were significantly higher than those of the control group (t =8.706,7.840,all P <0.05);NSE in blood and cerebrospinal fluid of HFMD complicated encepha-litis group were significantly higher than those of HFMD group (t =6.945,7.134,all P <0.05).S100B in blood and cerebrospinal fluid of HFMD complicated encephalitis group were significantly higher than those of the control group (t =9.443,10.031,all P <0.05);S100B in blood and cerebrospinal fluid of HFMD complicated encephalitis group were significantly higher than those of HFMD group (t =8.552,9.206,all P <0.05).TNF -α,NSE and S100B levels in blood and cerebrospinal fluid of HFMD complicated encephalitis group were positively correlated (r =0.803, 0.794,0.856,all P <0.05);TNF -αlevels in blood and cerebrospinal fluid of HFMD group was positively correla-ted (r =0.743,P <0.05).Conclusion Detection of TNF -α,NSE and S100B levels in blood and cerebrospinal fluid can assist to diagnose children with HFMD,and NSE and S100B can be used as specific indicators of HFMD complicated encephalitis.
ABSTRACT
BACKGROUND:In previous studies, the dehydrothermal cross-linking method was modified by the authors to improve the degradation property of colagen scaffolds. The cross-linking time was increased from 24 to 48 hours, and the cross-linking temperature increased from 105 to 115℃. OBJECTIVE:To verify the anti-degradation ability of colagen scaffolds prepared using the modified dehydrothermal cross-linking method and to obtain the optimal efficacy of the scaffolds on damaged tissue repair and regeneration. METHODS: Highly-purified type I colagen scaffolds with native triple helix structure were prepared and subjected to three different dehydrothermal cross-linking conditions: 105℃ for 24 hours, 105℃ for 48 hours and 115℃ for 24 hours. Material samples, 1 cm×1 cm, were implanted subcutaneously into the rat dorsum. The specimens were harvested at 3 days, 14 days and 42 postoperative days folowed by fixation and histological analysis using hematoxylin-eosin staining. RESULTS AND CONCLUSION:No untoward foreign body and immunological reactions were observed in any groups. In the group of 105℃ for 48 hours, the scaffold retention and degree of pore openness were better than the other two groups at 14 days after scaffold implantation (P < 0.05). These findings indirectly suggest that the anti-degradation ability of colagen scaffolds can be strengthened under certain dehydrothermal cross-linking conditions: the cross-linking time is increased from 24 to 48 hours.
ABSTRACT
Aim To investigate the roles of FFJ-5 in human breast cancer MCF7 cells and drug-resistant MCF7/DOX cells and to explore its mechanisms. Methods MTT assay was used to detect the effect of FFJ-5 on MCF7 and MCF7/DOX cell proliferation and sensitivity of doxorubicin in MCF7/DOX cells.West-ern blot was used to investigate the effect of FFJ-5 on expression of EGFR,p-EGFR,Akt,p-Akt,PKM2, cleaved caspase-3,cleaved PARP and P-gp.DNA lad-der analysis was performed to determine the effect of FFJ-5 on genomic DNA.RT-PCR was performed to de-tect the influence of FFJ-5 on multidrug resistance gene MDR1 mRNA levels.Results The results showed that FFJ-5 inhibited the growth of MCF7 ,inhibited the expression and activity of EGFR and Akt,and conse-quently reduced the expression of PKM2 in MCF7 cells;FFJ-5 activated caspase-3 and induced genomic DNA fragmentation;FFJ-5 also inhibited the growth of MCF7/DOX cells and enhanced the anti-tumor activity of doxorubicin in MCF7/DOX cells.Conclusion The results suggest that FFJ-5 could inhibit MCF7 cell growth and induce MCF7 cell apoptosis through inhibi-tion of EGFR-Akt-PKM2 pathway and activation of ap-optosis-related factors caspase-3 , meanwhile FFJ-5 could also reverse the resistance of MCF7/DOX.