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Objective To investigate how microRNA 24 (miR-24) regulates the development of lung cancer. Methods The levels of miR-24 in 30 cases of lung cancer were detected using real-time PCR, and the relationship between miR-24 levels and overall survival was analyzed. After overexpression or silencing of miR-24 in A549 lung cancer cells, the effect on cell proliferation was observed by the MTT assay. Transwell assays were carried out to observe the effect of miR-24 on cell migration. The effects of miR-24 on the expression of cyclin-dependent kinase (CDK) 4/6 and matrix metalloproteinase (MMP) 2 in A549 cells were examined by Western blotting. Results Survival analysis showed that patients with low miR-24 expression had a shorter survival time. The MTT-based viability assay revealed that overexpression of miR-24 inhibited A549 cell proliferation. Cell proliferation was promoted when miR-24 was inhibited. In the Transwell assay, overexpression of miR-24 significantly inhibited the A549 cell migration, and cell migration increased when miR-24 was inhibited.Western blotting analysis revealed that overexpression of miR-24 could inhibit CDK4/6 and MMP2 production in A549 cells. Inhibition of miR-24 was associated with increased production of CDK4/6 and MMP2 in A549 cells. Conclusion miR-24 can inhibit the proliferation and migration of A549 lung cancer cells.
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Objective To explore the effect of noscapine on the biological function of A549 cells and its underlying mechanism. Methods Concentrations of 10 μmol/L and 20 μmol/L of noscapine were used on the A549 cells. The effect of noscapine on their proliferation was observed via the MTT assay. Western blotting and real time PCR were used to detect the effects on Cyclin D1, BCL-2, and MMP2. The Hoechst 33258 staining assay was used to observe the effects of noscapine on the apoptosis of A549 cells. The metastasis of A549 cells was detected by using the Transwell assay. Results The MTT assay showed that the rate of inhibition of cell proliferation after treatment with 10 μmol/L and 20 μmol/L noscapine were (32.98±1.09) ％ and (49.56±3.98) ％, with a significant difference between the values (P ＜ 0.05). Hoechst 33258 staining evinced that noscapine promoted apoptosis, while the Transwell assay displayed that noscapine inhibited A549 cell metastasis. Western blotting and real time PCR indicated that the expression of Cyclin D1, BCL-2, and MMP2 in A549 cells was inhibited by noscapine. Conclusion Noscapine can inhibit the growth and metastasis of the lung cancer cell line A549 and promote the apoptosis of A549 cells. In conclusion, noscapine can potentially be used as a chemotherapeutic drug for lung cancer.
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Objective:To establish an atomic absorption spectrophotometric method for the determination of 6 heavy elements in-cluding lead,cadmium,mercury, arsenic, copper and chromium in iron filings and ferric ammonium citrate in order to control the quality and ensure people's daily medicine safety.Methods:The determination method for lead,cadmium,chromium,copper,arse-nic and mercury was a graphite furnace method, a graphite furnace method, a graphite furnace method, a flame method, a hydride method and a cold steam absorption method,respectively. Results:The concentration of lead within the range of 0-50 ng·ml-1had a good linear relationship with the absorbance(r=0.999 8),and the average recovery was 98.9%(RSD=2.3%,n=6). The concen-tration of cadmium within the range of 0-4 ng·ml-1had a good linear relationship with the absorbance(r=0.999 1),and the average recovery was 90.5%(RSD=1.5%,n=6). The concentration of chromium within the range of 0-50 ng·ml-1had a good linear rela-tionship with the absorbance(r=0.999 7),and the average recovery was 96.1%(RSD=1.1%,n=6). The concentration of copper within the range of 0-800 ng·ml-1had a ood linear relationship with the absorbance (r=0.999 9), and the average recovery was 97.5%(RSD=1.2%,n=6). The concentration of arsenic within the range of 0-16 ng·ml-1had a good linear relationship with the absorbance (r=0.999 0),and the average recovery was 93.4%(RSD =1.1%, n =6). The concentration of mercury within the range of 0-18 ng·ml-1had a good linear relationship with the absorbance(r=0.999 2),and the average recovery was 93.2%(RSD=3.8%,n=6). Conclusion:The method with good repeatability is accurate,which can be used for the quality control of iron filings and ferric ammonium citrate.
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Objective:To establish an HPLC method to determine the contents of ephedrine hydrochloride, (R, S)-goitrin, lae-trile,chlorogenic acid,licorice glycosides and glycyrrhizic acid in Xiao'er Kechuanling granule.Methods:The chromatography condi-tions were as follows:an Agilent Eclipse XDB-C18(250 mm×4.6 mm,5 μm) column was used,and acetonitrile-0.1% phosphoric acid was applied as the mobile phase. The flow rate was 1.0 ml·min-1with gradient elution. The wavelength was 207 nm for ephed-rine hydrochloride and laetrile,237 nm for glycyrrhizic acid,licorice glycosides and chlorogenic acid and 245 nm for (R,S)-goitrin. Results:The linear range of ephedrine hydrochloride was 2.423-96.920 μg·ml-1(r =0.999 2), and the average recovery was 100.1% (RSD=0.30%,n=6). The linear range of (R, S) -goitrin was 1.920-76.798 μg·ml-1(r=0.999 9), and the average recovery was 99.86% (RSD=1.14%,n=6). The linear range of chlorogenic acid was 2.396-92.891 μg·ml-1(r=0.999 9),and the average recovery was 98.57% (RSD =0. 75%,n =6). The linear range of amygdalin was 1.982-79.279 μg·ml-1(r =0.999 8),and the average recovery was 99.67% (RSD=0.59%,n=6). The linear range of glycyrrhizin was 2.136-85.440 μg· ml-1(r=0.999 9),and the average recovery was 98.57% (RSD=0.69%,n=6). The linear range of glycyrrhizic acid was 2.432-97.260μg·ml-1(r=0.999 9),and the average recovery was 98.57% (RSD=0.11%,n=6). Conclusion: The method is accu-rate and reliable,which can be used for the quality control of Xiao'er Kechuanling granule.
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OBJECTIVE:To explore the effects of short-term application of atorvastatin combined with ezetimibe on efficacy and related indicators of patients with primary nephrotic syndrome with hyperlipidemia. METHODS:Data of 50 patients with prima-ry nephrotic syndrome with hyperlipidemia were retrospectively collected and divided into combination group and control group ac-cording different treatment,25 cases in each group. All patients received low-salt,low-fat ,high-quality protein,giving prednisone 1 mg/(kg·d),po,qd,combined with anticoagulation,diuretic,anti-infection,taking cytotoxic drugs if necessary. Based on it, control group received Atorvastatin calcium tablet 20 mg before going to bed,qd;combination group received Atorvastatin calcium tablet(the same dosage and usage with control group)+Ezetimibe tablet 10 mg,qd. They were treated for 2 weeks. Lipid-lowering efficacy and low-density lipoprotein(LDL-C),triglyceride(TG),cholesterol(TC),high-density lipoprotein(HDL-C),24 h uri-nary protein (M-TP),serum albumin,alanine aminotransferase (ALT),aspartate aminotransferase (AST),serum creatinine and blood urea nitrogen before and after treatment in 2 groups were observed and the incidence of adverse reaction was recorded. RE-SULTS:There was no significant differences in the total effective rate of Lipid-lowering in 2 groups(P>0.05). After treatment, LDL-C,TC and HDL-C in 2 groups were significantly lower than before,Alb in combination group and ALT in 2 groups were sig-nificantly higher than before,with statistical significance(P0.05). And there was no significant difference in the TG,M-TP,AST,serum creatinine and blood urea nitrogen before and after treatment(P>0.05). CONCLUSIONS:Atorvastatin combined with ezetimibe can improve the blood lipid of patients with primary nephrotic syndrome with hyperlipidemia,while showing similar efficacy and safety with atorvastatin alone in a short term.
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OBJECTIVE:To establish the quality standard of Corydalis tomentella. METHODS:The medicinal material was identified in respects of property,microscopic characteristics and TLC. The contents of moisture,total ash and water soluble ex-tract were determined. The content of dehydrocavidine was determined by HPLC. The determination was performed on Agilent C18 column with mobile phase consisted of acetonitrile-phosphate buffer solution(containing 20 mmol/L monopotassium phosphate,10 mmol/L diethylamine,0.1% phosphoric acid)(28:72,V/V)at the flow rate of 1.0 mL/min. The detection wavelength was set at 347 nm,and the column temperature was 35 ℃. The sample size was 10 μL. RESULTS:The original plant is perennial herbs. The me-dicinal material often shrank into a ball. The main root was in conical shape;the rhizomes and roots were distinctly chapped;leaves curled and broken,and flowers were yellowish-white. The pollen grains were round-like in shape;square and columnar crys-tals were found;there were a large number of nonglandular hairs. The bordered pit,threaded and reticulate catheter were found;wood fiber was also found. TLC spots were clear and well-separated. The content of moisture were 7.5%-18.5%,total ash were 20.5%-26.2%,and extract were 29.9%-46.4%. The linear range of dehydrocavidine were 0.04008-2.4048 μg(r=0.9999);RSDs of precision,stability and reproducibility tests were lower than 2.0%. The recoveries were 95.6%-102.5%(RSD=2.3%,n=9). CONCLUSIONS:The established standard can be used for quality evaluation of C. tomentella.
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OBJECTIVE:To establish a method for the residues determination of Pb,Cd,Cu,As and Hg in Codonopsis pilo-sula,and evaluate the quality evaluation of C. pilosula of Pingshun County in Shanxi province. METHODS:Microwave diges-tion-inductively coupled plasma mass spectrometry was adopted with KED scanning model,RF power was 1 550 W,sampling depth was 5.0 mm,plasma gas(argon)flow rate was 16.0 L/min,helium partial pressure was 0.1 mbar,argon gas was 0.6 mbar, the vacuum degree of 5×10-8 mbar,branch turbopump speed was 1 000 hz,sampling cone aperture was 1.0 mm,skimmer aperture was 0.5 mm,the spray chamber temperature was 2.7 ℃,the data collection was repeated 3 times. RESULTS:The linear range was 0-20 ng/ml for Pb(r=0.999 3),0-10 ng/ml for Cd(r=0.998 5),0-250 ng/ml for Cu(r=0.998 8),0-20 ng/ml for As(r=0.999 0) and 0-1.0 ng/ml for Hg(r=0.997 9);RSDs of precision,stability and reproducibility tests were lower than 3.0%;recoveries were 95.80%-100.20%(RSD=1.85%,n=6),94.50%-98.00%(RSD=1.26%,n=6),98.52%-102.43%(RSD=1.60%,n=6), 94.90%-98.70%(RSD=2.29%,n=6)and 96.00%-101.00%(RSD=1.84%,n=6);the limits of detection were 0.021 0,0.003 4, 0.043 7,0.115 6 and 0.005 6 ng/kg,respectively. Pb,Cd,Cu,and As were detetcted,and Hg was not detected,the range of total contents was 7.185 2~12.558 0 mg/kg. CONCLUSIONS:The method is simple with good precision,stability and reproducibility, and can be used for the residues determination of Pb,Cd,Cu,As and Hg in C. pilosula;heavy metal residues in C. pilosula in Shanxi Pingshun county does not exceed limit values of national and industry standards.
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Objective To explore the correlation between the glucose variability and the severity of acute isolated traumatic brain injury(TBI). Method According to the inclusion/exclusion criteria,a total of 125 cases of acute isolated TBI admitted in Department of Neurosurgery of China Medical University from July 2012 to June 2015 were included. According to Glasgow coma scale(GSC),the patients were divided into five groups including control(GCS 15),mild(GCS 13?14),moderate(GCS 9?12),severe(GCS 6?8),and extra?severe(GCS 3?5)groups. Blood glucose control(including relief of the stress and the application of insulin)were carried out immediately. The average,standard deviation,and variation co?efficient of blood glucose of all groups were recorded at admission,48 hours and 3?7 days after hospitalization. The clinical records and glycemic in?dex were compared among different groups and during different periods,so as to analyze the relationship of the variability of glucose and the duration of hyperglycemia with the severity of TBI and the effects of glycemic intensive care management. Results The results of Kruskal Wallis test and Mann?Whitney Utest showed that the average,standard deviation,and variation coefficient of glucose in the extra?severe group and the severe group were statistically higher than those in the control group(P1). Conclusion The glucose variability in acute isolated TBI patients could be considered as the index of the severity of TBI.
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To explore detection and adequacy evaluation of erythrocyte glutathione S transferase [GST] in hemodialysis patients on circular toxin levels, this paper divided 84cases of long-term hemodialysis end-stage patients into 33 cases of adequate hemodialysis group [spKtN>1.3] and 51 cases of inadequate hemodialysis group [spKtN<1.3] according to urea index value of unit chamber model [spKt/V]. Take the other 50 cases of healthy physical examination people for control group, compare and analyze related clinical and biochemical indexes differences of three groups. The level of hemodialysis group GST, creatinine, high sensitivity C-reactive protein [hs-CRP], transferrin saturation [TSAT], parathyroid hormone [PTH], interleukin-2,6,8 [IL-2,6,8] and tumor necrosis factor-a [TNF-a] was significantly higher than the control group [P<0.05], and GST, IL-2, 6, 8, TNF-a level of inadequate hemodialysis group was significantly higher than adequate hemodialysis group [P<0.05]. Pearson's relevant analysis showed that GST and spKt/V, IL-2, IL-6, IL-8, TNF-a have positive correlation [P<0.05] and had no correlation with creatinine, hs-CRP, TSAT, PHT [P>0.05]. There was 23patients spKtN>1.3 after adjusting the dialysis solution for 5lcases of inadequate hemodialysis patients, GST level after the adjustment was significantly lower than before the adjustment, but still higher than adequate dialysis eroup. It concludes that the maintenance of hemodialysis patients' level has certain relevance on spKt/V and associated inflammatory factors. Through the determination, GST can effectively response the adequate hemodialysis, which has a guiding significance on adjusting blood dialysis solution in clinic
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BACKGROUND: Estrogen exerts a negative regulatory role in adipogenic differentiation of adipose stem cells, but there is no report concerning estrogen effects on adipogenic differentiation of adipose stem cells from the adipose capsule of kidney after long-term cryopreservation. OBJECTIVE: To explore the impact of estrogen on adipogenic differentiation of fresh adipose-derived mesenchymal stem cells from the kidney adipose capsule or those after long-term cryopreservation. METHODS: Passage 3 long-term cryopreserved and fresh adipose-derived mesenchymal stem cells from the kidney adipose capsule were divided into four groups, al of which were induced to adipogenic cells by induced fluid: fresh cells + 10-7 mol/L estrogen, cryopreserved cells + 10-7 mol/L estrogen, fresh cells and cryopreserved cells groups. Oil red O staining and adipogenic quantitative detection were performed at 14 days after induced adipogenic differentiation. RESULTS AND CONCLUSION: There were no differences in the morphology and arrangement between the cryopreserved and fresh cells. Both cryopreserved and fresh cells expressed CD29 and CD 44, but did not express CD31. Intracel ular lipid droplets were observed after adipogenic differentiation by oil red O staining, and the cells were positive for oil red O staining. The adipogenic volume comparison among the four groups was detected on day 14 after adipogenic differentiation, and the absorbance values showed significant difference between the fresh cells and fresh cells + estrogen groups, as wel as between the cryopreserved cells and cryopreserved cells + estrogen groups, but no difference between the fresh and cryopreserved cells groups. It is proved that low-dose estrogen can inhibit the adipogenic differentiation of long-term cryopreserved adipose-derived mesenchymal stem cells from the kidney adipose capsule; however, there is no significant difference between passage 3 long-term cryopreserved and fresh adipose-derived mesenchymal stem cells from the kidney adipose capsule in adipogenic differentiation.
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Objective To investigate the clinical and pathological features of rectal carcinoid and factors influencing the prognosis. Methods Clinical data of 29 cases with postoperative pathology identified diagnosis of rectal carcinoid from May 1998 to May 2008 in the two hospitals were retrospectively reviewed. Endoscopic submucosal resection of the tumor was conducted in 5 cases,local resection in 14 cases, local expanded resection of the tumor in 4 cases, transsacral local wide resection in 2 cases, radical operation in 4 cases. Results Median age at treatment was 32～71 (54±11) years. Median follow-up was (61±4) months (3 months-10 years), follow-up rate was 76%. During a follow-up, there were no cases with recurrence among the 13 patients with tumor size<1 cm, 1 case recurred in the 5 patients with turmor size between 1 to 2 cm, and 3 cases died from postoperative liver metastasis among the 4 patients with tumor size > 2 cm. The 5-, 10-year disease-specific survival rates were 87% and 80% respectively. Conclusions Surgery is the best therapy for rectal carcinoid, the choice of operative mode must be made according to the size,infiltration of the tumor, the condition of infiltrated lymph node and hepatic metastasis.
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BACKGROUND: The stem cell transplantation achieved progress in treating acute kidney injury. However, cell transplantation in treating chronic renal damage, as well as the protection mechanism, remains poorly understood.OBJECTIVE: To observe the changes of urine protein and renal function, as well as the effects of bone marrow mononuclear cells on expression of transforming growth factor β1 (TGF-β1) and connective tissue growth factor (CTGF) in chronic renal damage rats.DESIGN, TIME AND SETTING: The randomized controlled animal experiment was performed at the Experimental Animal Center of General Hospital of Shenyang Military Command from May to October 2008.MATERIALS: Totally 30 female SD rats, with SPF grade, were randomly divided into the control, model and cell transplantation groups, with 10 animals in each group. Additional 10 SD rats were used to prepare bone marrow mononuclear cells.METHODS: Chronic renal damage models were prepared by modified hepatic resection, and 1.0 mL mononuclear cells were injected at 10 weeks after model preparation, with 1×10~8 cells per animal. Rats in the model group were injected same volume of physiological saline.MAIN OUTCOME MEASURES: The 24 hour urinary protein excretion quantity and renal function changes, histopathological change of renal tissues, as well as the expression of TGF-β1 and CTGF.protein excretion, urea nitrogen and creatinine levels (P < 0.01), which especially greater in the model group (P < 0.01 or P <expressions of TGF-β1 and CTGF were notably enhanced in the model and cell transplantation groups (P < 0.01), but the enhancement extent was larger in the model group (P < 0.01).CONCLUSION: Bone marrow mononuclear cells can decrease levels of urine protein excretion, blood urea nitrogen and creatinine, inhabit the TGF-β1 and CTGF expression, relief pathological changes, eventually, protect the kidney of subtotally nephrectomized rats.