1.
J Biosci
;
2001 Dec; 26(5): 635-40
Article
in English
| IMSEAR
| ID: sea-111330
ABSTRACT
The cDNA for pre-pro-Concanavalin A (pre-pro-ConA) was cloned into the cytoplasmic expression vector pKK233-2 to give rise to pCONEXP2 which was used to express the lectin precursor. Pre-pro-ConA is stable and is not transposed and ligated to form the mature protein. No signal peptide removal is observed. The solubility of pre-pro-ConA could not be increased by guanidine hydrochloride denaturation/dilution treatment.
Subject(s)
Blotting, Western , Cloning, Molecular , Concanavalin A/chemistry , Fabaceae/chemistry , Plant Lectins , Protein Precursors/chemistry , Recombinant Proteins/chemistry , Solubility
2.
Article
in English
| IMSEAR
| ID: sea-111176