[Correlation of hormonal disorders with menstrual disorders]

Menstrual disorders are including of metruragy, oligomenorrhea, polymenorrhea, dysmenorrheal and amenorrhea. Hormonal disorders can be the cause of such abnormalities. This study was designed to evaluate, the correlation between serum thyroid and sex hormones level, with menstrual disorders. This descriptive - analytical study carried out on 110 women with menstrual disorders without anatomical disorders referred to private clinic during 2007-08. In third day of menstrual cycle, sample blood was taken for determination of LH, FSH, PRL, TSH, T3, T4 and Testosterone. Laboratory tests were performed by ELISA method. The 42.7% of women with menstrual disorders were in age group lower than 25 years. Oligomenorrhea with 42.7% was the major menstrual disorder and amenorrhea was the lowest with 4.5%. The most hormonal disorders was related to T3 [46.3%], LH [44.5%] and testosterone was the lowest with 5.4%. 11 cases [10.0%] have presented with hirsutism. According to findings of this study T3 and LH are the common hormonal disorders in menstrual abnormalities

Serum level of lactate dehydrogenase, homocystein, hemoglobin and platelet in preeclampsia

Pre-eclampsia affects approximately 5-8% of pregnant women. The aim of this study was to compare the serum level of Lactate dehydrogenase [LDH], Homocystein, Hemoglubin and platelet in pregnant women diagnosed as pre-eclampsia and a normal group in Gorgan city, Northeastern Iran from 2007-2008. In this case control study, 50 cases of pre-eclampsia were compared with the control group women hospitalized in Dezyani hospital. Pre-eclampsia criteria were: Blood pressure more than or equal to 140/ 90 mm hg and Proteinuria greater or equal to 300 mg/ 24 hours urine sample in the third trimester. Hemoglobin, platelet, LDH and hemocystein were measured. Data were analyzed by the mean of SPSS-14 program and Chi-2 or t-student were used. The difference of BMI and family incomes was significant between two groups [P-value<0.01]. LDH level was not statistically different between healthy and pre-eclamptic individuals. Six cases [12%] in controls and 9 cases [18%] in pre-eclamptic group had thrombocytopenia [P-value>0.01]. Hemocystein level was more than normal range in five patients with pre-eclampsia [P-value<0.001]. In this study, hemocystein level was significantly higher in pre-eclampsia patients but LDH, hemoglobin and platelet level had no significant difference

[Antibacterial activity of 7 species of medicinal plants on bacteria isolated from UTI patients in golestan province]

Urinary tract infection [UTI] is extremely prevalent in the world. Despite treatment with antibiotics, recurrency is common in the patients and there is potential for developing antibiotic resistance. The aim of this study was to investigate the antibacterial activity of ethanolic extracts of 7 medicinal plants on pathogenic bacteria causing Urinary Tract Infection. Plants were collected from their natural habitats in Golestan province [North of Iran]. Antibacterial effects of ethanolic extracts were analyzed by disc diffusion method. Also the Minimum inhibitory concentration [MIC] of the extracts was determined by the microdilution Broth. The results showed that Berberis vulgaris was the most effective extract with 29.4 mm diameter of inhibition zone on gram positive bacteria specially S. epidermidis. A. calcoaceticus, S. aereus, S. epidermidis, and S. saprophyticus were the most sensitive bacteria with maximum inhibition zone of 20.1, 26, 29.4, 28.5 mm, respectively. P. aeroginosa, C. freundii, K. pneumonia, and P. mirabilis were the most resistant bacteria in this research. Also extracts had better antibacterial effect in amount of 100 mg/ml. The lowest MIC values was measured in Berberis vulgaris with 0.09 mg/m10 against S. epidermidis. Gram positive bacteria were more sensitive than gram negative bacteria. These plants in native's traditional medicine have been used by rural people as antiseptic for the treatment of UTI. In addition, Our results showed that Berberis vulgaris and Hypericum perforatom were have strong antibacterial effects. In addition to Traditional uses of these plants in Golestan province for the treatment of UTI symptoms, invitro evaluation and clinical trials for their effects to treat UTI is suggested for future research

Possible role of TORCH agents in congenital malformations in Gorgan, northern Islamic Republic of Iran

This descriptive, cross-sectional study was carried out to explore the frequency of contamination with TORCH agents in neonates with congenital malformations in a referral centre in Gorgan city, Islamic Republic of Iran. Blood samples were taken from 64 neonates and their mothers over a 20-month period in 2003-04. Serologic tests showed that 4/64 infants born with congenital malformations [6%] had positive IgM antibody titres for Toxoplasma gondii [2 cases], rubella virus [1 case] and cytomegalovirus [1 case]. IgM was positive in 9/63 mothers [14%], also for T. gondii [3 cases], rubella virus [3 cases] and cytomegalovirus [3 cases]. No cases of herpes simplex virus type II or Treponema pallidum were found

[ antibacterial effects of 20 herbal plants on methicillin resistant and sensitive s.aureus in Golestan provience]

Staphylococcus aurues is on important cause of community and hospital- aquired infections. Caused by methicillin or oxacillin- resistant s.aureus [MRSA] are mainly nosocomial and are increasingly from many countries word wide. Many attempt have been made by the reasearchers to find new compounds as a subsitute for this antibiotics. The aim of this study was to investigate the antimicrobial activity of alcoholic extracts of 20 medical plants species of Golestan provience on clinical and standard strains of MRSA and MSSA and comprative and detect the best medical plant. In this study the compounds of the plant were extracted by percolation method and the effect of ethanolic extract of 20 Iranian medical plants against methicillin resistant and methicillin sensitve strains were assessed by disc diffusion method and each test were repeated 3 times and mean inhibition zone were recorded and then, the minimum inhibitory concentration [MIC] of the extracts, that show good inhibition zone in disc diffusion method, was determined by the micro broth dilution method. The results of antibacterial activity of the ethanolic extracts of 20 plants revealed that, the ethamlic extracts of 8 plants have the best effect on strains and the maximum mean inhibition zone was 22.4 mm and the lowest MIC of plants was 0.01 mg/ml. The result of this study indicate that, ethanolic extract of Eucalyptus. Global, Peganum.hermla, Punica.granatum, Berberis.vulgaris, Ttamarixaphylla, Nnigella.sativa, Hypericum.perforatum AND, Artemisia. Herbaalba have the best antibacterial effect against MRSA andMSSA. The result obtained from these plants might be considered sufficent for further study

[Antagonistic effect of lactic bacteria present in yoghurt against pathogenic bacteria]

Probiotics have been defined as live microorganisms that confer a health effect on the host when consumed in adequate amounts.Lactic bacteria play an important role in production and maintenance of the fermented and probiotic products. The aim of this study was to determine the efficacy of lactic bacteria, which isolated from yoghurt against Gastrointestinal pathogenic microorganisms in the Golestan province in North of Iran. In this descriptive study we used 96 strains which belong to 12 species of Lactobacillus and 5 species of Lactococcus, that isolated from home made yoghurt, and their antimicrobial effect on 7 species of important intestinal pathogenic microorganisms were examined. After growing the Lactic bacteria in broth media centrifuged and filtred the suspension and used the supernatant for study.The effect of supernatant against intestinal pathogenic microorganisms were assessed by Disc diffusion and pour in well method and each test were repeated 3 times and mean inhibition zone were recorded Lactobacillus casei and Lactococcus lactis showed better effect than other strains.The maximum light zone diameter was 18 milimeter. Maximum and minimum inhibitiry effect has seen in Yersinia enterocolitica and Bacillus cereus. Both Lactobacillus and Lactococcus strains had a proper inhibitory effect on the intestinal pathogenic bacteria but Lactobacillus strains that present in yoghurt showed better effect. This inhibitory effect was more obvious on the Yersinia enterocolitica

[Mycobacterium marinum infection in Caviar fishes and fishermans in Ashorada of Golestan province in north of Iran]

Mycobacterium marinum is the etiologic agent of fish Mycobacteriosis and fish tank granuloma in human in this study our objective was to determine of the fish tank granuloma in Fishermen and Mycobacterium marinum infection in Caviar fishes in Ashorade, in the Golestan province of Iran south east Caspian Sea border. Any suspected lesions in 387 subjects of Fishermen and 113 autopsy samples from gills of fish of Caviar were obtained and cultured in Lowenstein Jensen media. The mycobacterial species were determined by conventional biochemical tests. No fish tank granuloma was proved in human, but 11 [9.73%] strains of Mycobacteria were isolated from Caviar fishes, that only 2 strains [1.76%] belong to M.marinum. This study showed that was low prevalence any cases of fish tank granuloma and infection due to M.marinum in Caviar fish in this Region

Environmental mycobacteria in areas of high and low tuberculosis prevalence in the Islamic Republic of Iran

This research compared the numbers and types of different Mycobacterium species in soil samples taken from 2 areas of Golestan province, Islamic Republic of Iran, 1 with a high prevalence of tuberculosis and 1 with a low prevalence. From 220 samples, 91 grew positive cultures [41.4%] and 161 different strains were diagnosed. The most common species isolated were Mycobacterium fortuitum, M. flavescens and M. chelonae. The frequencies of environmental Mycobacterium in the low-prevalence area were much higher than in the high-prevalence area, perhaps due to different environmental factors

prevalence of infected stones in the urinary tract and their relationship to urease positive bacteria

To determine the prevalence of infected stones in the urinary tract and their relationship to urease positive bacteria and whether a positive urine culture signifies infected stone profile. This study was conducted on 168 patients [116 males and 52 females] with a mean age of 37 years. The analysis was performed on: [1] Urine specimen before operation, [2] Urine around the stone before stone retrieval and [3] Stones. Urine and stone culture was performed on blood agar [BA] and Eosin Methylene Blue [EMB] for gram staining, Brucella agar enriched with vitamin K and Thioglycolate medium for anaerobics and uroplasma urealyticum inside PPLO culture. Urease was determined on urea broth and urea agar media. Chemical analysis of the stone was done by Merck kit. Seven cases of culture were positive for infected stones [64%] while 6 cases of non-infected stones grew microorganisms [3.8%]. Infected stones comprised 6.5% of all the stones. Proteus mirabilis and Pseudomonas aeruginosa were the most commonly encountered bacteria [18%]. Urease positive bacteria were found to be 54.5% in the infected stones and 6% in the non-infected stones. Urine and stones samples showed that only 14.5% had the same type of bacteria. Other factors besides bacteria play a role in stone formation in the urinary tract and infected stones can be produced in non-infected urine also