ABSTRACT
<p><b>OBJECTIVE</b>To explore the dose-effect relationship between premature chromosome condensation induced by Calyculin A and irradiation dose.</p><p><b>METHODS</b>The human peripheral blood was irradiated by (137)Cs gamma radial. The irradiation dose included 0, 0.5, 1.0, 2.0, 3.0, 4.0, 5.0 Gy. The premature chromosome condensation induced by Calyculin A was observed, and dyed by centromeric banding.</p><p><b>RESULTS</b>There was the quadratic relation between the total aberration, fragment, dicentric+centric ring (dic+r) ration and irradiation dose.</p><p><b>CONCLUSION</b>Premature chromosome condensation induced by Calyculin A can be used as a biodosimetry.</p>
Subject(s)
Female , Humans , Male , Young Adult , Cell Line , Chromosome Aberrations , Radiation Effects , Chromosome Banding , Chromosomes , Radiation Effects , Dose-Response Relationship, Radiation , Gamma Rays , Lymphocytes , Radiation Effects , Oxazoles , PharmacologyABSTRACT
<p><b>OBJECTIVE</b>To explore the relationship between DNA repair in vitro and in vivo after irradiation, and to describe the curves of DNA repair which can improve the accuracy of radiation dose estimation.</p><p><b>METHODS</b>The DNA double-strand break in lymphocytes of human and mouse was detected using neutral single cell gel electrophoresis (SCGE) after radiation and the curves of DNA repair individually were estimated, which were compared later.</p><p><b>RESULTS</b>Along with the time lapsing, the DNA repair of human peripheral blood and mice increased significantly and the residual damage decreased gradually, which showed significant time-effect relationship. The curve of DNA repair in vitro of human lymphocytes presented the same log model as that of mouse DNA repair in vivo. The curve showed as followed respectively: Mice: Y(TM) = 55.8256 - 10.792 lnX (R(2) = 0.629, P < 0.01) and Y(OTM) = 25.4173 - 4.5273 lnX (R(2) = 0.661, P < 0.01); Human: Y(TM) = 30.242 7 - 7.383 6 lnX (R(2) = 0.686, P < 0.01) and Y(OTM) = 17.9772 - 3.9125 lnX (R(2) = 0.752, P < 0.01).</p><p><b>CONCLUSION</b>The curve of DNA repair in vitro of human lymphocytes could be considered in biodosimetry estimation because the process of DNA repair in vitro could display the repair level and speed of DNA double-strand break in vivo.</p>