ABSTRACT
Feline immunodeficiency virus (FIV) was first isolated in 1987 from a cat with an acquired immunodeficiency syndrome (AIDS)-like disease. Since then, FIV has been subject of intensive research. Perturbation in cytokine production observed in human immunodeficiency virus infection (HIV) is paralleled in the FIV-infected cat. Interferon gamma (IFN-gamma) is a type 1 lymphokine that exert protective effects during infection through upregulation of cellular immunity and phagocytic functions. The present study was carried out to examine the expression of IFN-gamma in a feline T-lymphoid cell line (Fel-039) infected with FIV as well as the viral replication in these cells after addition of recombinant-type feline IFN (rIFn). We found a marked inhibition of IFN-gamma release in Fel-039 cells infected with FIV which might be pivotal for high viral replication. Infection of Fel-039 cells with FIV resulted in an increase of the reverse transcriptase (RT) activity in the culture supernatant. When the cells were cultured in the presence of rIFN a significant dose-dependent inhibition of RT activity of FIV was detected without cytotoxicity. On the basis of these in vitro results, we suggest that IFN therapies aimed at restoring depleted level of this important cytokine in FIV infected T-cells make this compound a promising candidate for development of suitable drugs for AIDS treatment.
Subject(s)
Animals , Cats , Immunodeficiency Virus, Feline , Interferon-gamma , T-Lymphocytes , Cell Line , RNA-Directed DNA Polymerase , Feline Acquired Immunodeficiency Syndrome/metabolism , T-Lymphocytes , Immunodeficiency Virus, Feline/physiology , Virus ReplicationABSTRACT
A one-step enzyme linked sandwich immunoassay using Silicone rods coated with rabbit anti-human thyroglobulin anti-Tg) immunoglobulin G (Fab') conjugated with beta-D-galactosidase was established for the measurement of thyroglobulin in human sera. The volume of serum needed for the assay was 2 mul The sensitivity of the assay was 1.52 amol/tube, corresponding to O.5 ng/ml. The precision was proven by coefficients of variation: intra-assay, 7.0 to 9.1 per cent: inter-assay, 5.3 to 7.4 per cent. The correlation between this EIA and RIA was O.91, p < O.O1.
Subject(s)
Humans , Animals , Rabbits , Autoantibodies/blood , beta-Galactosidase/metabolism , Immunoglobulin G/analysis , Thyroglobulin/blood , Immunoenzyme Techniques , Immunoglobulins , Neoplasm Metastasis/diagnosis , Rabbits/immunology , Sensitivity and Specificity , SiliconesABSTRACT
An enzymeimmunoassay (EIA) for H-TSH (human thyrotropin) in dried blood on filter paper using an anti-H-TSH conjugate with ß-D-galactosidase and tubes coated with an anti-H-TSH was performed fo the screening program for detection of congenital hypothyroidism. The blood volume needed in this assay was 8.7 µl. The precision was evaluated by coefficients of variance within and between assays: 11.86 percent and 14.36 percent for H-TSH levels of 18.5 µU/ml and 35 µU/ml. A good correlation was observed between H-TSH concentration measured by EIA and RIA (r=0.91)