[Efficacy of Clotrimazole, Nystatin and Povidone Iodine in traetment of patients with vaginal candidiasis]

Vaginal candidiasis is one of the most frequent infections of the female genital tract in different countries. The goal of this study was to evaluate the efficacy of clotrimazile, nystatin and povidone iodine [betadine] in treatment of vaginal candidiasis in patients referred to gynecologic clinics of Mashhad. This is a clinical trial study on wemon referring to gynecologic clinics in Ghaem, Imam Reza, Imam Zaman and Ali - e - bn - e - Abi taleb centers, for vaginal discharge. Vaginal candidiasis was confirmed by direct fresh smear, Papanicolao staining, gram staining and culture. Among 300 cases, considering inclusion and exclusion criteria, 130 pateints suffering from vaginal candidiasis were selected. Each received either vaginal tablets of clotrimazole, nystatin or vaginal gel of betadine randomly. Among 300 patients suffering from vaginitis symptoms, candidiasis was confirmed only in 43% of the cases, most of them between 25-35 years old. Clotrimazole was more effective than betadine clinically. There was no significant difference between nystatin, clotrimazole or betadine in treatment of patients. Betadine side effects were more prominant compared to clotrimazole and nystatine. It is concluded that compared to betadine, clotrimazole and nystatin are safer in treatment of vaginal candidiasis and because of shorter duration of treatment, usage of clotrimazole is more reasonable in treatment of vaginal candidiasis compared to nystatin and betadine

[Preparation of fetal calf serum alternatives and their effects on growth and secretion of hybridoma cell lines]

Providing fetal calf serum [FCS] alternatives as cell culture supplements is an important field of research to compensate for the FCS supply shortage.This study focused on preparation of fetal calf serum alternatives and their effects on growth and secretion of hybridoma cell lines.Outdated human platelet units undergo extraction for its growth factors to be obtained. Human AB blood group plasma was also converted to serum and its growth effect was compared to FCS, hypoxanthine-thymidine [HT] and RPMI1640 as cell culture media and supplement. Cell growth indices were preliminary counting of cells, confluency as surface area of plates filled with cells, and titration of monoclonal anti-A and anti-B blood group antibodies collected from cultured mouse hybridoma cells. Statistical analysis including one sample t-test, logarithmic multiple regression curve fit, and factor analysis was done by SPSS v12 software. The four nutritional supplements of [1] human serum AB [AB], [2] human platelet extract [PLT], [3] equal mixture of AB and PLT [ABP], and [4] fetal calf serum as cell culture were examined on mouse hybridoma anti-A and anti-B monoclonal antibody producer cell lines for cell growth indices and compared with the same indices on RPMI1640 media. The growth-stimulating effects in descending order of values were [1] ABP5%, [2] FCS10%, [3] ABP10%, [4] AB5%, [5] AB10%, [6] PLT5%, [7] ABP20%, [8] PLT10%, [9] PLT20%, and [10]HT; but AB20% inhibited growth of mentioned hybridoma cell lines. The titer of anti-A and anti-B monoclonal antibodies produced by cultured hybridoma on 5 and 10 percent concentration of AB, PLT and ABP compared to FCS5-10% at descending order were [1] PLT5%, [2] PLT10%, ABP5%, ABP10%, AB10%, and [3] AB5%. In general FCS had the following effects on curves of cell growth: [1] the highest increase on slope of multiplication [ascending] phase, [2] the highest increase on slope of death [descending] phase, and [3] the lowest duration of stationary phase. Then, FCS can be appropriate for growth of cells at initial low cell count. Human serum AB, human platelet extract, and equal mixture of both at optimum concentrations [these supplements at high concentrations killed cells] compared to FCS showed [1] decreased slope of multiplication phase, [2] decreased slope of death phase, and [3] increased duration of stationary phase. Thus, AB and PLT may be suitable for continuous cell culture systems in which cell survival during longer times is required. Factor analysis was introduced as a model to evaluate kinetics of cell growth at different supplements