Antibacterial study of Acacia nilotica [linn.] and Syzygium cumini [linn.] two medicinal plants

Antibacterial activity of ethyl acetate extracts of nine different parts of two ethnomedicinal plants; Acacia nilotica [L.] and Syzygium cumini [L.], were evaluated against four bacterial strains including Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus and Streptococcus pneumoniae using well diffusion method. Different zones of inhibition were observed with the ethyl acetate extract of leaves, bark, fruits and roots of both plants at 0.1 mL of 100 mg/mL concentration and compared with same concentration of streptomycin as reference antibiotic. MIC and MBC for each part of plants were also calculated at 0.0-55 microg/mL concentration. Over all it was observed that among nine ethyl acetate extracts of selected plants, seven extracts inhibited the growth of E. coli, S. pneumoniae and K. pneumoniae, while eight inhibited S. aureus

Study of folk recipes and ethnomedicinal uses of thirty medicinal plants of district umerkot, Sindh, Pakistan

A survey on the folk medicinal uses and recipes of thirty medicinal plants was carried out in district Umerkot, Sindh, Pakistan. Since a long time the people of this area have been using medicinal plants and their different parts for the cure of a variety of illnesses. Since the knowledge about the traditional uses of these medicinal plants and their recipes of the area remained intact for exploration by the pharmacognosists, ethnobotanists, ethnopharmacologists, pharmacists and chemists. The folk uses and traditional recipes of thirty medicinal plants are therefore documented which are typically used in the healing of many health problems and diseases by the people of district Umerkot. Through this study, the medicinal plants are documented on the basis of their folk recipes and ethnomedicinal uses to demonstrate their efficacy to treat the diseases of humans and animals

Spectrophotometric and liquid chromatographic determination of dopamine from pharmaceutical preparations using acetyl acetone as derivatizing reagent

Dopamine [DA] has been determined by spectrophotometry and by liquid chromatography as derivatives of acetylacetone [AA]. The liquid chromatography of DA was carried out in the presence of hydroxyproline [HP], octopamine [OP] and tyramine [TY]. The separation was obtained from Phenomenex C-18 column 5pm [150 x 4.6 mm id] by isocratic elution with methanol: acetonitrile: water [45: 6: 49v/v/v], UV detection was at 320nm. Linear calibration curves were obtained for each with 2.5-40.0 micro gml-1 with detection limits in the range 1.8-80ng injection-1 [10 micro l]. The dopamine was determined in pharmaceutical preparations with coefficient of variation [CV] within 0.7-1.5%