ABSTRACT
Objective To investigate the correlation among serum galactose immunoglobulin of quantitative change, total IgG and CIC(circulating immune complexes)levels in patients with schizophrenia.Methods Test the number changed of PVL affinity type Ig in 80 patients with schizophrenia and 80 controls′serum with the PVL affinity ELISA method.The specimens without central nervous system organic disease,heart,liver,kidney diseases, and autoimmune diseases are collected from March to June 2015 in Hospital 261 of PLA′s hospitalized patients.The total of cases is 80.The cases of male are 52,and the cases of female are 28.The age is from 19 to 60,and the average of age is(27.2 ±6.2).Control group is selected from health checkers from the same petiod.The cases of male are 52,and the cases of female are 28.The age is from 19 to 61,and the average of age is(27.8 ±5.4).The relationships among serum PVL-Ig,total IgG and CIC levels in patient group and control group were investigated by rectilinear correlation analysis.Results The serum PVL-IgG level(3.128 ±0.531)(A value)in schizophrenia was significantly higher than control group(2.672 ±0.492)(A value),(t=3.846,P<0.01).PVL-IgM(0.351 ±0.082)(A value)and PVL-IgA(1.287 ±0.205)(A value)compared with the control group(0.362 ±0.073,1.203 ± 0.216)(A value), there was no difference(t=0.241,0.6013;P>0.05).There was positive correlation between serum PVL-IgG and CIC in patients(r=0.423 1,P<0.01).There was no significant correlation with IgG,IgM and IgA.Conclusions People with schizophrenia have an excess of PVL-IgG expression,which maybe participated in the pathological process of immunological injury.PVL-IgG may be one of the molecular mechanisms of patients′CIC stranded or cleared of obstacles.
ABSTRACT
Objective To establish a platform for enrichment and purification of core fucose glycosylation and analyse its immu‐nity properties .Methods Serum immunoglobulin G(IgG) was seperated and purified by using protein G cross‐linked sepharose ,and core fucose glycosylation components of IgG were enriched and purified by using lens culinaris agglutinin (LCA)affinity chromatog‐raphy ,meanwhile ,the isoforms of LCA‐IgG were determined by using Western blotting .Results The purification rate of serum IgG was 62% and yield rate of LCA‐IgG was 4 .59% .Serum IgG1 ,IgG2 and IgG3 were identified except IgG4 in patients with schizo‐phrenia ,while serum IgG1 ,IgG2 ,IgG3 and IgG4 were all identified in healthy individuals .Conclusion A method for enrichment and purification of core fucose glycosylation in patients with schizophrenia have been successfully established and distribution character‐istics of the LCA‐IgG isoforms have been determined ,which could provide a new insight into immune pathomechanism of schizo‐phrenia .
ABSTRACT
Objective To investigate the correlation between the levels of serum immunoglobulin core fucosylation and humoral immune parameters in patients with schizophrenia and its immunological significance .Methods The levels of serum lens culinaris agglutinin(LCA)-IgM ,LCA-IgG ,LCA-IgA ,IgM ,IgG ,IgA ,circular immune complex(CIC) in patients with schizophrenia(n= 46) and health people(n=47) were determined .Their correlations were investigated by linear regression analysis .Results There was a positive correlation between the LCA-IgA and IgG in healthy people(r=0 .311 7 ,P<0 .05) .However ,there was a negative correla-tion between LCA-IgG and IgA in patients with schizophrenia(r=0 .323 6 ,P<0 .05) .In addition ,there were positive correlations between LCA-IgM ,LCA-IgG ,LCA-IgA and CIC(r=0 .354 8 ,r=0 .189 8 ,r=0 .479 7 ,P<0 .05) .Conclusion Increased fucosyla-tion levels of immunoglobulin were detected in patients with schizophrenia ,which may play important roles in immunological inju-ries of central nervous system in schizophrenia .