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【Objective】 To analyze nutrition-related factors that affect the prevalence of myopia in adolescents, in order to provide reference for primary prevention of myopia. 【Methods】 A stratified sampling method was used to select 385 adolescents from Zhengding County in October 2021. Adolescents in this study took vision testing, physical examination and completed a questionnaire survey. One-way analysis of variance and lasso regression were used to screen the variables, and Logistic regression was used to determine the possible influencing factors of myopia. Factor analysis was adopted to extract the dietary patterns of adolescents in Zhengding County, then the association between dietary patterns and myopia was analyzed. 【Results】 A total of 385 adolescents were surveyed, with the prevalence rate of myopia of about 68.6%. Multivariate analysis revealed that fried food(OR=8.480, 95%CI:1.058 - 67.971) was a risk factor for myopia, while intake of milk and dairy products(OR=0.994, 95%CI:0.991 - 0.999), soybeans and nuts(OR=0.997, 95%CI:0.994 - 0.999), no myopia in either parent(OR=0.312, 95%CI:0.115 - 0.845), physical education class 4 times per week(OR=0.269, 95%CI:0.074 - 0.984) were statistically associated with a lower risk of myopia(P<0.05). Three dietary patterns was extracted through factor analysis, including diversified dietary pattern, soy-hybrid dietary pattern, and snack and beverage dietary pattern. Logistic analysis results indicated that soy-hybrid dietary pattern(OR=0.85, 95%CI:0.73 - 0.99,P<0.05) was statistically associated with myopia. 【Conclusions】 The problem of myopia among adolescents in Zhengding County is more serious. Increasing the intake of milk and dairy products, soybeans and nuts, reducing the intake of fried foods, and adjusting the overall dietary structure should be recommended in order to prevent the development of myopia in adolescents.
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Objective@#To analyze the characteristics of influenza virus detection in an influenza outbreak in schools, so as to provide a strategic basis for the treatment of influenza outbreaks in schools.@*Methods@#A total of 1 702 samples were collected from 52 school influenza outbreaks reported in Linyi City in 2021-2022. The samples were divided into 3 types according to different symptoms during the management of the epidemic [group A:influenzalike illness (ILI) group; group B:mild illness group; group C:close contacts group]. Rt-PCR was used to detect influenza virus nucleic acid in the collected samples. The detection rate of influenza virus in the outbreaks was analyzed by χ2 test.@*Results@#In total, 1 071 samples (62.93%) tested positive for influenza virus nucleic acid. Among them, 610 out of 726 samples (84.02%) were detected in group A, while 331 out of 634 samples (52.21%) were detected in group B. In group C, 130 out of 342 samples (38.01%) tested positive. The differences were statistically significant (χ2=260.71, P<0.01). In group A, males had a detection rate of 80.83% for influenza virus nucleic acid, compared to 91.36% for females. For group B, the rates were 53.31% for males and 50.87% for females. In group C, males had a rate of 30.72%, while females had a rate of 43.92%. Statistical significance for gender differences was observed only in groups A and C (χ2=12.67, 6.25, P<0.05). According to the days of onset, the detection rates of influenza virus nucleic acid among patients with onset 0-6 days were 56.30%, 74.49%, 89.35%, 86.23%, 69.67%, 62.75%, 34.33%, respectively, and the difference was statistically significant (χ2=128.27, P<0.01).@*Conclusions@#Mild cases and close contacts are likely key factors contributing to the prolonged emergence of new cases within classrooms during school influenza outbreaks. The progression of influenza symptoms is related to the risk of transmission.
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Objective To investigate the efficacy and safety of Huoluoxiaolingdan formula combined with ginkgolide injection in the treatment of ischemic stroke patients with blood stasis blockage syndrome.Methods The ischemic stroke patients with blood stasis blockage syndrome who were treated in Suzhou First People's Hospital from June 2017 to August 2019 were randomly divided into test group and control group.The control group was given ginkgolide injection,and the test group was given Huoluoxiaolingdan formula additionally.Both groups were treated for 2 weeks.The efficacy and adverse drug reactions of the two groups were evaluated,and the changes of clinical scores,hemorheology,blood lipids and inflammatory factors before and after treatment were compared between the two groups.Results A total of 194 patients were included,there were 97 cases in each group.The total effective rate of test group was 91.75％,which was significantly higher than that of control group(79.38％)(P＜0.05),and the incidence of adverse reactions was significantly lower than that in the control group(P＜0.05).After treatment,NIHSS,CSS and ASS in both groups were lower than before treatment(P＜0.05),while BI was higher than before treatment(P＜0.05).The levels of ηp,ηb,Fib and EEP in test group were significantly lower than those in control group(P＜0.05).HDL-C level of test group was significantly higher than that of control group,TC,LDL-C and TG levels were lower than that of control group(P＜0.05).The levels of IL-4 in test group were significantly higher than those in control group,and the levels of IL-1 β,IL-8 and TNF-α were lower than those in control group(P＜0.05).The above indexes in the test group was significantly better than that in control group(P＜0.05).Conclusion Huoluoxiaolingdan formula combined with ginkgolide injection can significantly improve the efficacy of patients with blood stasis blockage syndrome during the recovery period of ischemic stroke,improve clinical symptom score,correct abnormal hemorheology,reduce blood lipid level,inhibit the progression of inflammation,and alleviate adverse reactions,which has high clinical application value.
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Objective To investigate the correlation of serum atresia zonule protein 1(ZO-1)and anti-β2-glycoprotein 1 antibody(aβ2-GP1)levels with infarct lesion extent and degree of carotid atherosclerosis in pa-tients with cerebral infarction.Methods A total of 103 patients with cerebral infarction treated in Baoding No.1 Central Hospital from January 2020 to January 2023 were selected as the study group,and 51 healthy subjects in the same hospital during the same period were selected as the control group.Patients with cerebral infarction were divided into large infarction group(infarct lesion extent>50 cm2),middle infarction group(infarct lesion extent as 10-50 cm2),and small infarction group(infarct lesion extent<10 cm2)according to the infarct lesion extent.According to the results of carotid ultrasound,the patients with cerebral infarction were divided into plaque stage group,thickening stage group and normal vessel wall group.Enzyme-linked im-munosorbent assay was used to detect serum ZO-1,aβ2-GP1 levels,and the levels of ZO-1 and aβ2-GP1 in each group were compared.Spearman correlation analysis was used to analyze the correlation between serum ZO-1,aβ2-GP1 levels and the infarct lesion extent and the degree of carotid atherosclerosis in patients with cerebral infarction.Results The study group had a significantly lower serum level of ZO-1 and a significantly higher serum level of aβ2-GP1 than the control group(P<0.05).Among the 103 patients with cerebral in-farction,there were 32 cases in the large infarction group,34 cases in the middle infarction group,and 37 cases in the small infarction group.The small infarction group had a significantly higher level of ZO-1 and a signifi-cantly lower level of aβ2-GP1 than the medium infarction group and large infarction group(P<0.05).The middle infarction group had a significantly higher level of ZO-1 and a significantly lower level of aβ2-GP1 than the large infarction group(P<0.05).Among 103 patients with cerebral infarction,there were 29 cases in the plaque stage group,38 cases in the thickening stage group,6 cases in the normal vessel wall group.The plaque stage group had a significantly lower serum level of serum ZO-1 and a significantly higher level of aβ2-GP1 than the thickening stage group and normal vessel wall group(P<0.05).The thickening stage group had a significantly lower serum level of ZO-1 and a significantly higher serum level of aβ2-GP1 than the normal ves-sel wall group(P<0.05).Spearman correlation analysis showed that the serum level of ZO-1 was negatively correlated with the degree of carotid atherosclerosis and the extent of infarction(P<0.05),and the serum level of aβ2-GP1 was positively correlated with the degree of carotid atherosclerosis and the extent of infarc-tion(P<0.05).Conclusion The levels of serum ZO-1 and aβ2-GP1 in patients with cerebral infarction are significantly correlated with the degree of carotid atherosclerosis and the extent of infarction,which have po-tential value in the diagnosis,treatment and prognosis of patients with cerebral infarction.
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【Objective】 To investigate the relationship between maternal oral health behavior during pregnancy and premature birth. 【Methods】 A total of 248 preterm and 700 non-preterm pregnant women who delivered in Northwest Women’s and Children’s Hospital in Shaanxi Province from January to December 2022 were included in case group and control group, respectively. General demographic characteristics and oral health behavior during pregnancy were collected by questionnaires, and the oral health impact profile-14 (OHIP-14) was used to assess the oral health related life quality of pregnant women. Logistic regression model was used to analyze the relationship between maternal oral health behavior during pregnancy and premature birth. 【Results】 During pregnancy, maternal frequent eating after brushing teeth (OR=1.62, 95% CI: 1.03-2.57), unused fluoride toothpaste (OR=2.03, 95%CI: 1.25-3.05), late visit to the doctor for oral discomfort (OR=1.41, 95%CI: 1.02-1.96), brushing teeth less than twice one day (OR=1.77, 95%CI: 1.13-2.78) or less than 3 minutes each time (OR=1.52, 95%CI: 1.09-2.11), and elevated OHIP-14 score (OR=1.07, 95%CI: 1.04-1.10) increased the risk of premature birth. 【Conclusion】 Poor oral health behavior during pregnancy may increase the risk of premature birth. Therefore, more efforts should be made to publicize oral health knowledge and guide pregnant women to establish good oral health habits so as to improve oral health and promote maternal and child health.
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Objective:This study aimed to evaluate the effect of enhanced external counterpulsation(EECP)on left ventricular function in elderly patients with coronary slow flow phenomenon(CSFP)using two-dimensional speckle tracking echocardiography(2D-STE).Methods:This prospective case-control study included 30 patients aged ≥60 years with no stenotic lesions in the coronary arteries but with slow blood flow phenomenon in more than one major coronary artery who were treated at the Department of Geriatrics, Qilu Hospital, Shandong University, between December 2017 and December 2018, and were divided into a medication group with 16 participants and a medication plus EECP group with 14 participants, using the numerical lottery method.Patients in the group treated with EECP received 6-week 36-h EECP therapy in addition to lifestyle modification and drug treatment.Fourteen patients with normal coronary blood flow served as the control group.Conventional echocardiography and 2D-STE were used to evaluate changes in left ventricular function in the CSF patients before and after drug treatment and EECP.Results:Compared with the control group before treatment, patients in the drug treatment group and the drug treatment plus EECP group showed a decrease in mitral annular early diastolic velocity( P<0.01), an increase in the ratio of peak mitral early diastolic blood flow velocity to the mean peak mitral annular early diastolic velocity( P<0.05), and a decrease in left ventricular longitudinal strain during systole( P<0.01), the longitudinal systolic myocardial strain rate( P<0.01)and the early diastolic longitudinal peak strain rate( P<0.01).There was no statistically significant difference in values from conventional echocardiographic parameters before and after treatment in CSF patients of the medication group(all P>0.05).In the group receiving EECP, there were statistically significant differences in pre-and post-treatment values in ventricular septal early diastolic velocity[(6.22 ± 0.64)cm/s vs.(6.69 ± 0.44)cm/s], lateral wall early diastolic velocity[(8.01±0.68)cm/s vs.(8.41±0.29)cm/s], mitral valve to mitral annulus early diastolic peak velocity ratio[(10.51±1.38) vs.(9.74±0.37)], longitudinal left ventricular systolic strain[(-16.21±0.46)% vs.(-16.80±0.48)%], left ventricular systolic longitudinal strain rate[(-1.29±0.03)s -1vs.(-1.35±0.04)s -1], and early diastolic longitudinal strain rate[(1.35±0.03)s -1vs.(1.40±0.03)s -1](t-values were -3.70、-2.74、2.23、10.25、12.30、-19.15, all P<0.05). Conclusions:2D-STE can evaluate subclinical myocardial dysfunction early and quantitatively in elderly patients with CSF, and objectively reflect changes in left ventricular function before and after clinical intervention with EECP.
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Objective:To observe the rehabilitation effect of Baduanjin combined with elastic band training on elderly patients with sarcopenia. Methods:From January 2019 to January 2021, 120 elderly sarcopenia patients in our hospital who met the inclusion criteria were divided into two groups according to the random number table method, with 60 in each group. The control group was given conventional treatment intervention for sarcopenia, and the observation group was Baduanjin exercises combined with elastic band training on the basis of the control group. Both groups were intervened for 12 weeks. The skeletal muscle index (SMI), muscle grip strength, short physical performance battery (SPPB) and Modified Barthel Index (MBI) were used to compare between two groups before and after the intervention. Results:After treatment, the SMI index [(6.77 ± 1.03) kg/m 2vs. (6.35 ± 1.12) kg/m 2, t=2.14], muscle grip strength [(23.06 ± 3.48) kg vs. (19.41 ± 3.79) kg, t=5.50], SPPB score [(9.12 ± 2.24) vs. (7.85 ± 2.13), t=3.18] and MBI score [(82.43 ± 20.75) vs. (64.36 ± 19.42), t=4.93] of the observation group were significantly higher than those in the control group ( P<0.05). After treatment, the distribution of muscle activity function in the observation group was significantly better than that of the control group ( Z=-2.28, P=0.023). Conclusion:Baduanjin combined with elastic band training can improve the muscle function and quality of daily activities in elderly patients with sarcopenia.
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Objective:To analyze the influencing factors of attention deficit hyperactivity disorder (ADHD) in children and construct a Nomogram prediction model.Methods:A total of 5 409 children aged 7 to 16 from 5 schools in Xinjiang were investigated by using SNAP-Ⅳ assessment scale and influencing factors questionnaire.Least absolute shrinkage and selection operator (LASSO) regression and multivariate Logistic regression were used to analyze and investigate the influencing factors of ADHD in children, and then Nomogram prediction model was established. Results:(1)The detection rate of ADHD was 7.3%.(2) The LASSO- Logistic regression model showed that the history of febrile convulsions ( OR=5.97, 95% CI: 3.52-9.86), the history of epilepsy disease ( OR=11.86, 95% CI: 7.83-17.89), the history of head trauma disease ( OR=10.0, 95% CI: 7.27-13.71), mother′s delivery method ( OR=2.53, 95% CI: 1.99-3.23), mother′s education level ( OR=2.26, 95% CI: 1.45-3.67), mother′s smoking more than 1 year ( OR=12.65, 95% CI: 8.30-19.34), whether the family environment is quiet ( OR=1.27, 95% CI: 1.00-1.63), and the education method of beating and scolding ( OR=3.05, 95% CI: 2.13-4.31) was an indepen-dent risk factor for children with ADHD; (3)The Nomogram prediction model was built and verified by Bootstrap for 1 000 samples.The C-index was 0.81(95% CI: 0.78-0.83), suggesting that the Nomogram prediction model has good prediction ability, accuracy, and distinction.Decision curve analysis (DCA) of the clinical decision curve suggested that patients with Nomogram model with a predictive probability threshold greater than 0.2 had a higher clinical net benefit. Conclusions:The detection rate of ADHD was 7.3%, which was higher than the national average.The Nomogram prediction model drawn here can provide individualized ADHD risk predictions for children based on the history of hyperthermia, epilepsy, and head trauma, maternal mode of childbirth, maternal education level, maternal education level, maternal smoking for more than 1 year, quiet family environment, and scolding education methods.
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Objective:To analyze the associated symptoms of progressive symmetrical erythema keratosis (PSEK) and the related literature was reviewed.Methods:Two Mongolian PSEK families in the dermatology department of the People′s Hospital of Inner Mongolia Autonomous Region from 2016 to 2017 were collected, and 40 complete PSEK families and 156 sporadic cases at home and abroad published since 1980 to 2020 were searched by using the database to analyze the concomitant symptoms of PSEK.Results:A total of 40 complete PSEK families were included, including 714 cases. The incidence of PSEK was 57.38% in foreign countries and 37.42% in China; The most common concomitant symptoms abroad were palmoplantar keratosis (PPK), followed by nail changes, neurological symptoms, dysplasia, combined with variable erythema keratosis (EKV), and the concomitant symptoms were more serious; The most common concomitant symptoms in China were nail changes, followed by PPK, damp hyperhidrosis, pruritus, pain and some skin diseases, and the concurrent symptoms were mild.Conclusions:PSEK has many associated symptoms and the molecular genetic mechanism is still unclear. It is necessary to conduct a more comprehensive and in-depth study and understanding of the disease through the development of sequencing technology and the expansion of clinical cases.
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Objective:To evaluate the effect of total intravenous anesthesia combined with transversus abdominis plane block (TAPB) and rectus sheath block (RSB) in patients undergoing laparoscopic total hysterectomy.Methods:One hundred and twenty patients who required a laparoscopic total hysterectomy under total intravenous anesthesia from April 2019 to June 2020 in General Hospital of Eastern Theater Command were selected. The patients were divided into 4 groups by random digits table method with 30 cases each: total intravenous anesthesia group (C group), total intravenous anesthesia combined with TAPB group (T group), total intravenous anesthesia combined with RSB group (R group) and total intravenous anesthesia combined with TAPB and RSB group (S group). The narcotic (propfol and remifentanil) dosage, changes of resting visual analogue score (VAS) within 48 h after operation, pressure number of analgesia pump within 48 h after operation, anesthesia satisfaction score and incidence of emergence agitation were compared among 4 groups.Results:The remifentanil dosage, propfol dosage and pressure number of analgesia pump within 48 h after operation in S group were significantly lower than those in T group, R group and C group: (1.09 ± 0.23) mg vs. (1.49 ± 0.21), (1.47 ± 0.26) and (1.48 ± 0.23) mg, (543.53 ± 41.78) mg vs. (618.96 ± 37.21), (598.67 ± 37.86) and (607.87 ± 36.93) mg, (3.52 ± 2.03) times vs. (5.47 ± 1.83), (6.63 ± 2.08) and (9.77 ± 2.16) times, the anesthesia satisfaction score was significantly higher than that in R group and C group: (18.13 ± 3.22) scores vs. (21.43 ± 2.42) and (21.44 ± 2.56) scores, and there were statistical differences ( P<0.05). The resting VAS extubation and 12, 24, 48 h after operation in S group was significantly lower than that in C group and R group, the resting VAS 12, 24 and 48 h after operation was significantly lower than that in T group, and there were statistical differences ( P<0.05). The incidence of emergence agitation in S group was significantly lower than that in C group: 6.67% (2/30) vs. 26.67% (8/30), and there was statistical difference ( P<0.05). Conclusions:TAPB combined with RSB can reduce the narcotic dosage, reduce the incidence of emergence agitation and relieve perioperative pain in patients undergoing laparoscopic total hysterectomy. The effect is better than that of total intravenous anesthesia and total intravenous anesthesia combined with TAPB or RSB.
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Objective: To analyze the clinical characteristics, treatments, and outcomes of lung cancer patients with NTRK mutations. Methods: The clinical data of patients with pathologically confirmed primary lung cancer and the NTRK mutation in next-generation sequencing (NGS) admitted to Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology between October 2016 and November 2019 were retrospectively analyzed. The Kaplan-Meier method and the Log-rank test were used for the univariate survival analysis. Results: A total of 28 patients were enrolled. 27 patients were diagnosed as stage with point mutations or copy number amplifications of NTRK, and 1 patient was diagnosed as stage IIIC with AEN-NTRK3 (A1:N18) fusion. The univariate analysis showed that the median progression-free survival (PFS) was related to the pathological pattern (adenocarcinoma vs. squamous carcinoma: 9.4 months vs. 2.5months, P0.05, respectively). Contrastingly, the median PFS of EGFR-TKIs treatment for patients with EGFR mutant lung cancer and concomitant NTRK1 mutation was significantly longer than patients with concomitant NTRK3 mutation (12.4 months vs. 3.0 months, P<0.05). Conclusions: Patients with NTRK mutant lung adenocarcinoma had a longer PFS than patients with lung squamous carcinoma when they received first-line therapy. EGFR mutant patients with concomitant NTRK3 mutation had a poorer prognosis than patients with concomitant NTRK1 mutation when they received EGFR-TKIs treatment. Therefore, concomitant NTRK3 mutation may be one of the poor prognostic factors of EGFR mutant lung cancer.
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Objective:To screen out and analyze the key genes of follicular lymphoma (FL) according to transcriptome data in Gene Expression Omnibus (GEO) database.Methods:Transcriptome datasets GSE32018 and GSE55267 were collected by using GEO database. R software was used for variance analysis to screen differentially expressed genes. FunRich 3.13 software was used to analyze common differential genes. The biological processes and pathways were analyzed with Cytoscape 3.7.2 software to screen potential genes related to the pathogenesis of FL. By analyzing clinical data from Oncomine database for survival analysis, the screened differential genes were verified.Results:A total of 141 up-regulated genes and 199 down-regulated genes were identified by differentially analyzing GSE32018 and GSE55267 datasets. Finally, 12 key genes including CXCL8, KRT19, CYCS, CDKN3, SFN, RRM2, FN1, APOE, CXCL12, VWF, GATA3 and TIMP1 were screened out; CYCS, CXCL8 and CXCL12 were closely related with the early survival rate of patients. Overexpression of CXCL12 and low expression of CYCS were found to be associated with poor prognosis of FL patients. CXCL8 expression was decreased in lymphoma tissues, but the relatively high expression of CXCL8 in survival analysis showed shortened overall survival, which might be related to the early development of FL. GO, KEGG and Reactome pathways were screened out including GO: 0001892, KEGG: 04115, R-HSA: 2559582, GO: 0060968, R-HSA: 6785807, GO: 0043627, GO: 0001936 and GO: 0043062.Conclusion:The selected genes CYCS, CXCL8 and CXCL12 may provide more effective biomarkers for the treatment of FL.
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Objective To investigate the effect of nimodipine combined with cerebrospinal fluid replacement on hemoglobin concentration,Toll-like receptor 4 (TLR4) expression level and cerebral vasospasm (CVS) in patients with CVS after aneurysmal subarachnoid hemorrhage (aSAH).Methods One hundred and twenty patients with CVS after aSAH admitted to the Department of Neurosurgery,the Sixth Medical Center of PLA General Hospital from May 2013 to May 2015 were selected.They were randomly divided into control group and observation group (n =60 in each group).The control group received conventional treatment and nimodipine infusion after embolization of the aneurysms,and the observation group underwent cerebrospinal fluid replacement by lumbar puncture on this basis.The clinical efficacy,Glasgow Coma Scale (GCS) scores,hemoglobin concentration and TLR4 expression levels before and after treatment,and adverse reactions were compared between the two groups.Results One month after treatment,the improvement rate of vasospasm in the observation group was significantly higher than that in the control group (86.7％ vs.60.0％;x2 =9.590,P =0.002).Three months after the treatment,the good rate of clirnical outcome (the modified Rankin Scale score 0-2) was significantly higher than that of the control group (88.3％ vs.58.3％;x2 =13.807,P＜0.001).Before treatment,there were no significant differences in hemoglobin concentration and TLR4 expression levels between the two groups;after treatment,the hemoglobin concentration and TLR4 expression levels of both groups were significantly reduced (P ＜0.05).Compared with the control group,the hemoglobin concentration (119.9 ± 19.8 g/L vs.137.6 ± 17.8 g/L;t =3.270,P =0.001) and TLR4 expression level (2.5 ± 1.2 vs.4.5 ± 1.5;t =8.060,P ＜0.001) in the observation group decreased more significantly.Multivariate logistic regression analysis showed that hypertension (odds ratio [OR] 5.19,95％ confidence interval [CI] 2.31-6.71),hyperlipidemia (OR 2.70,95％ CI 1.93-4.86),previous history of stroke or transient ischemic attack (OR 6.29,95％ CI 3.23-7.32),smoking (OR 4.80,95％ CI 2.18-6.19),and the TLR4 expression level before treatment (OR 3.28,95％ CI 2.87-6.93) were independently correlated with the lack of improvement in CVS,and cerebrospinal fluid replacement was independently correlated with CVS improvement (OR 0.40,95％ CI 0.14-0.89).There was no significant difference in the incidence of adverse reactions such as blood pressure drop,obstructive hydrocephalus and gastrointestinal hemorrhage betw een the observation group and the control group,but the incidence of delayed CVS (13.3％ vs.36.7％;x2 =7.510,P =0.006) and secondary cerebral infarction (8.3％ vs.31.7％;x2 =8.800,P =0.003) in the observation group were significantly lower than those of the observation group.Conclusion Nimodipine infusion combined with cerebrospinal fluid replacement by lumbar puncture affected the hemoglobin concentration and TLR4 expression levels,improved the CVS improvement rate,and significantly improved the clinical outcome in patients with CVS after aSAH.
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Objectives To study the significance of SATB2 expression in colon adenocarcinoma and its differential diagnosis function for ovarian metastatic adenocarcinoma.Methods Immunohistochemistry was used to detect the expression level of SATB2 in 130 cases of colon adenocarcinoma.The relationship between the positive rate of SATB2 expression in colon cancer and clinicopathological factors was studied.Forty-seven cases of pancreatic ductal adenocarcinoma,22 cases of cholangiocarcinoma,46 cases of gastric adenocarcinoma,and 53 cases of ovarian mucinous adenocarcinoma were studied respectively.Results The positive expression rate of SATB2 in 130 cases of colon adenocarcinoma is 73.8％.The SATB2 expression bears no correlation with gender,age,tumor size,location,histology type,lymph node metastasis,staging,local recurrence,distant metastasis,survival,Kras mutation,and microsatellite stability.The expression rate of SATB2 is significantly higher in well differentiated and moderately differentiated colon adenocarcinoma than that in poorly differentiated adenocarcinoma (x2 =12.804,P =0.002);the expression rate in the cases without tumor deposit is significantly higher than in cases with tumor deposit (x2 =6.485,P =0.011).There was no positive expression in all cases of pancreatic adenocarcinoma,cholangiocarcinoma,gastric adenocarcinoma,nor in ovarian mucinous adenocarcinoma.Conclusion The expression of SATB2 is associated with the differentiation of colon adenocarcinoma and the formation of tumor deposit.SATB2 can be used as an effective tumor marker for identifying colorectal cancer ovarian metastases.
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Objectives@#To study the significance of SATB2 expression in colon adenocarcinoma and its differential diagnosis function for ovarian metastatic adenocarcinoma.@*Methods@#Immunohistochemistry was used to detect the expression level of SATB2 in 130 cases of colon adenocarcinoma. The relationship between the positive rate of SATB2 expression in colon cancer and clinicopathological factors was studied. Forty-seven cases of pancreatic ductal adenocarcinoma, 22 cases of cholangiocarcinoma, 46 cases of gastric adenocarcinoma, and 53 cases of ovarian mucinous adenocarcinoma were studied respectively.@*Results@#The positive expression rate of SATB2 in 130 cases of colon adenocarcinoma is 73.8%. The SATB2 expression bears no correlation with gender, age, tumor size, location, histology type, lymph node metastasis, staging, local recurrence, distant metastasis, survival, Kras mutation, and microsatellite stability. The expression rate of SATB2 is significantly higher in well differentiated and moderately differentiated colon adenocarcinoma than that in poorly differentiated adenocarcinoma (χ2=12.804, P=0.002); the expression rate in the cases without tumor deposit is significantly higher than in cases with tumor deposit (χ2=6.485, P=0.011). There was no positive expression in all cases of pancreatic adenocarcinoma, cholangiocarcinoma, gastric adenocarcinoma, nor in ovarian mucinous adenocarcinoma.@*Conclusion@#The expression of SATB2 is associated with the differentiation of colon adenocarcinoma and the formation of tumor deposit. SATB2 can be used as an effective tumor marker for identifying colorectal cancer ovarian metastases.
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Objective@#To investigate the effect of nimodipine combined with cerebrospinal fluid replacement on hemoglobin concentration, Toll-like receptor 4 (TLR4) expression level and cerebral vasospasm (CVS) in patients with CVS after aneurysmal subarachnoid hemorrhage (aSAH).@*Methods@#One hundred and twenty patients with CVS after aSAH admitted to the Department of Neurosurgery, the Sixth Medical Center of PLA General Hospital from May 2013 to May 2015 were selected. They were randomly divided into control group and observation group (n=60 in each group). The control group received conventional treatment and nimodipine infusion after embolization of the aneurysms, and the observation group underwent cerebrospinal fluid replacement by lumbar puncture on this basis. The clinical efficacy, Glasgow Coma Scale (GCS) scores, hemoglobin concentration and TLR4 expression levels before and after treatment, and adverse reactions were compared between the two groups.@*Results@#One month after treatment, the improvement rate of vasospasm in the observation group was significantly higher than that in the control group (86.7% vs. 60.0%; χ2=9.590, P=0.002). Three months after the treatment, the good rate of clinical outcome (the modified Rankin Scale score 0-2) was significantly higher than that of the control group (88.3% vs. 58.3%; χ2 =13.807, P<0.001). Before treatment, there were no significant differences in hemoglobin concentration and TLR4 expression levels between the two groups; after treatment, the hemoglobin concentration and TLR4 expression levels of both groups were significantly reduced (P<0.05). Compared with the control group, the hemoglobin concentration (119.9±19.8 g/L vs. 137.6±17.8 g/L; t=3.270, P=0.001) and TLR4 expression level (2.5±1.2 vs. 4.5±1.5; t=8.060, P<0.001) in the observation group decreased more significantly. Multivariate logistic regression analysis showed that hypertension (odds ratio [OR] 5.19, 95% confidence interval [CI] 2.31-6.71), hyperlipidemia (OR 2.70, 95% CI 1.93-4.86), previous history of stroke or transient ischemic attack (OR 6.29, 95% CI 3.23-7.32), smoking (OR 4.80, 95% CI 2.18-6.19), and the TLR4 expression level before treatment (OR 3.28, 95% CI 2.87-6.93) were independently correlated with the lack of improvement in CVS, and cerebrospinal fluid replacement was independently correlated with CVS improvement (OR 0.40, 95% CI 0.14-0.89). There was no significant difference in the incidence of adverse reactions such as blood pressure drop, obstructive hydrocephalus and gastrointestinal hemorrhage between the observation group and the control group, but the incidence of delayed CVS (13.3% vs. 36.7%; χ2=7.510, P=0.006) and secondary cerebral infarction (8.3% vs. 31.7%; χ2=8.800, P=0.003) in the observation group were significantly lower than those of the observation group.@*Conclusion@#Nimodipine infusion combined with cerebrospinal fluid replacement by lumbar puncture affected the hemoglobin concentration and TLR4 expression levels, improved the CVS improvement rate, and significantly improved the clinical outcome in patients with CVS after aSAH.
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With the development and application of microarray and high-throughput sequencing technology, it is possible to genomi-cally scan breast cancer associated CNV and SNPs. Multiple researches showed that the frequent aberrance of 8q24 was associated with breast cancer. Oncogenes such as MYC, PSCA and breast cancer associated loci are located in 8q24, and frequent amplification of 8q24 was proposed to be related with breast cancer development and prognosis. This review summarizes the association of the 8q24 amplification and SNPs with breast cancer by correlated genes on this locus.
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Objective: To investigate the imaging features of primary adenoid cystic carcinoma (ACC) of the breast. Methods: In total, 31 patients were confirmed to have primary ACC in the breast based on histopathological findings from January 2012 to September 2018 in Tianjin Medical University Cancer Institute and Hospital. Mammography and ultrasonography findings of these patients were retrospectively analyzed according to the breast imaging-reporting and data system (BI-RADS) recommended by the American College of Radiology (ACR) and compared with pathological findings. Results: Mammography findings in 31 cases of ACC of the breast were as follows: 25 cases with masses, including 16 high-density masses (14 were categorized as BI-RADS 4C or 5, one as 4A, and one as 4B); 7 equal-density masses (5 were categorized as BI-RADS 4B, one as 4A,and one as 3); and 2 mixed-density masses (one was categorized as BI-RADS 4A and one as 2). The other 6 patients presented with focal asymmetric density. No calcification was found in all cases. Ultrasonography findings included hypoechoic mass, mixed-echo mass, and non-mass-like hypoechoic or heterogeneous echoic areas. A total of 22 patients presented with hypoechoic masses; of these, 14 showed typical ultrasonographic findings of breast cancer and their tumors were categorized as BI-RADS 4C or 5. Three patients presented with mixed-echo masses, of which two showed a mixed-echo mass containing hyper-echoic areas and one showed a complex cystic and solid echo mass; all tumors were categorized as BI-RADS 4B. The remaining 6 patients presented with non-mass-like hypoechoic or heterogeneous echoic areas; all were without a tendency to distribute along the direction of the breast ducts. Conclusions: The mammographic and ultrasonographic appearances of primary ACC of the breast are similar to those of general carcinoma, with no specificity. Some specific findings, such as irregular high-density masses containing low-or fatty-density areas with indistinct margins on mammography and mixed-echo masses containing hyper-echo or non-mass-like heterogeneous echoic areas not distributed along the breast duct on ultrasonography, have certain significance. The final diagnosis depends on histopathology.
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Objective@#To investigate the expression of immunomarkers CK7, CK20, CK17, CDX2, MUC1 and MUC2 in primary adenocarcinoma of the ampulla of Vater, to explore the role of these markers in the histopathologic subclassification of ampullary carcinoma; and to provide biologic basis for precision treatment of patients with different types of ampullary carcinoma.@*Methods@#Forty-two cases of primary ampullary carcinoma were collected at Peking University People′s Hospital, from 2012 to 2018 year. There were 22 males and 20 females. Aged range 42 to 88 years old, with mean aged (62±11) years. Among the patients, 6 was high differentiation, 19 median differentiation, and 17 low differentiation. Immunohistochemical studies on the expression of CK7, CK20, CK17, CDX2, MUC1 and MUC2 were performed in 42 cases of primary ampullary carcinoma. The relationship between different ampullary carcinoma subtypes and clinicopathologic survival data was analyzed using SPSS 16.0 statistical software.@*Results@#Three histopathologic subtypes were observed. Among 42 cases, 8(19.0%)were classified as intestinal subtype, which showed a positive expression rate of 8/8 for both CK20 and CDX2, and 5/8 for MUC2. Both CK7 and CK17 were weakly expressed in one case (1/8). No expression was observed for MUC1 in this subtype. Twenty-two (52.4%,22/42) cases were classified as pancreaticobiliary subtype, which showed a positive expression rate of 100.0%(22/22) for both CK7 and MUC1, and 90.9% (20/22) for CK17. No expression was observed for CK20, CDX2 and MUC2.The remaining 12 (28.6%) cases were classified as mixed subtype, which showed variable expression patterns. The expression frequencies of these 6 immunomarkers in different subtypes of ampullary carcinoma did not correlate with various clinicopathologic factors such as patient gender and age, tumor size, histologic differentiation, pancreatic and bile duct invasion, or the depth of duodenal invasion. However, stage Ⅲ+Ⅳ diseases were more commonly seen in pancreaticobiliary type (63.6%,14/22) than intestinal type (2/8) and mixed type (3/9; χ2=6.508, P=0.039). Follow-up data showed a trend of better survival rate for patients with intestinal subtype than those with mixed and pancreaticobiliary subtypes.@*Conclusions@#Ampullary carcinoma can be subclassified into three different subtypes using a panel of six immunomarkers, especially for the identification of subtypes of poorly differentiated carcinoma. CK7, CK17 and MUC1 are major markers of pancreaticobiliary subtype, whereas CK20, CDX2 and MUC2 are useful markers for intestinal subtype. The mixed subtype variably expresses these markers. The prognosis of patients with intestinal subtype appears better than that of pancreaticobiliary and mixed subtypes. Accurate subtyping of ampullary carcinoma is clinically important to patient management and prognosis assessment.
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Objective To investigate the effect of Hp infection on the inflammatory factors, gastrointestinal hormones and insulin resistance in patients with type 2diabetes mellitus.Methods A total of 86patients with type 2diabetes mellitus treated in a hospital from May 2014to October 2016were enrolled in the study.All patients underwent 13C urea breath test after admission, and according to the test results, patients were divided into the observation group, which was type 2diabetes mellitus with Hp infection (n=46) and the control group, which was type 2diabetes mellitus without Hp infection (n=40) .Inflammatory factors such as highsensitivity C reactive protein (hs-CRP) , serum interleukin 6 (IL-6) , tumor necrosis factorα (TNF-α) , gastrointestinal hormones such as serum gastrin (GAS) , somatostatin (SS) and fasting blood glucose (FPG) , fasting insulin (FINS) , insulin resistance index (HOMA-IR) were compared between the two groups, and the correlation of the observation indicators with Hp infection was explored.Results Levels of hs-CRP, IL-6, TNF-αand GAS, FPG, FINS and HOMA-IR in the observation group were significantly higher than those in the control group, while SS was significantly lower than that in the control group, the difference was statistically significant (P<0.05) .Further Sperman correlation analysis showed that hs-CRP, IL-6, TNF-α, GAS, FINS and HO-MA-IR were positively correlated with Hp infection (r=0.452, 0.350, 0.398, 0.389, 0.421, 0.568, P<0.05) , and SS was negatively correlated with Hp infection (r=-0.401, P<0.05) .Conclusion Hp infection is closely related to inflammatory reactions, gastrointestinal hormone disorders and insulin resistance in patients with type 2diabetes mellitus, and taking reasonable preventive measures is the key to delay the progression and deterioration of type 2diabetes mellitus.