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Objective To evaluate the effect of situational health education in glycemic control in type 2 diabetes patients.Methods A total of 98 patients with type 2 diabetes was randomly assigned to the control(n=49)and the study group(n=49).The control group received conventional diabetes education,while the study group received situational health education.Fasting blood glucose(FBG),2-hour plasma glucose (2 hPG),HbAlc and the self-care behaviors were observed at baseline and 1-year after the intervention.Paired t test was used for data analysis.Results FPG,2hPG,HbA1c and self-care behavior scores were not significantly different between the two group at baseline.After 1-year intervention,FPG,2hPG and HbA1c of the two groups were significantly decreased,and FPG,2hPG and HbA1c of the study group were significantly lower than those of the control group(t values were 2.71,3.35 and 2.90,respectively; all P＜ 0.05).In comparison with the control group,self-care scores of the study group were increased(t values were 7.36,6.51,5.24,5.07,6.57 and 6.77,respectively; all P＜0.05).Conclusion Situational health education could improve self-management and glycemic control of type 2 diabetic patients.
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Objective To explore the method to relieve fingertip pain in monitoring fingertip blood glucose.Methods A total of 130 newly admitted patients with type 2 diabetes received glucose monitoring 6 times daily at different time points.Blood sampling was taken from different sites including the central and top of middle finger pulp,central and side of ring finger pulp,top of middle finger with pressing the acupoint of Hegu at the same time or pressing acupoint of Hegu for 1 minute before blood taking.Pain degree was assessed by numeric rating scale after blood glucose taking.Pain scores were compared among the different methods.Results There were significant differences in pain scores between different methods (x2 =164.83,P＜0.05).Pain score was higher in sampling blood on the side of ring finger pulp,while the proportion of subjects with mild pain after pressing Hegu was increased significantly.There were significant differences in pain scores between subjects with versus without pressing Hegu (all P＜0.05).Pain degree was reduced more significantly by pressing Hegu for 1 minute before blood taking than pressing Hegu at the same time of sampling blood,and the former had a higher proportion of subjects with only mild pain than the latter (x2 =10.44,P＜0.01).There were no significant differences in pain scores between the other methods (all P＞ 0.05).Conclusions Pressing Hegu can effectively reduce fingertip pain in fingertip blood glucose monitoring,and pressing Hegu for 1 minute before blood taking is more effective.
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BACKGROUND: How to establish a stable in vitro culture system, including location of corneal limbal epithelial stem cells, in vitro sample harvest, in vitro culture, vector selection, as well as identification methods, play a key role in corneal limbal epithelial stem cells culture. OBJECTIVE: To culture the isolated rabbit corneal limbal epithelial stem cells and to identify the biological properties of cultured cells. METHODS: The primary rabbit cornel limbal epithelial stem cells were isolated and cultured with tissue inoculation using human amniotic membrane as vector. The growth features of cells were observed under an inverted microscope. The morphology of cells was observed by hematoxylin-eosin staining and a scanning electron microscope. Furthermore, the monoclonal antibody AE5 and P63 two-step immunohistochemical staining were used to identify limbal epithelial stem cell protein expression. RESULTS AND CONCLUSION: The rabbit corneal limbal epithelial stem cells could be successfully cultured and maintained a relatively high value-added potential in vitro. Rabbit corneal limbal epithelial stem cells cultured on the amniotic membrane pull netted cellular layer. The AE5 monoclonal antibody positive rate of primary cultured cells was about 5% and P63 monoclonal antibody positive up to 90%. AE5-positive rate increased and P63-positive rate decreased with the increase in the number of subculture. The rabbit limbal epithelial stem cells can be successful culture and amplified on human amniotic membrane in vitro by limbal tissue culture method. The cultured cells maintain the characteristics of corneal epithelial cells. The rabbit corneal limbal epithelial stem cells can form grafts on the amniotic membrane.
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OBJECTIVE:To establish a RP-HPLC method for the determination of Esculetine in Dinglian tablets.METHODS:The chromatographic column was Hypersil ODS C18(200mm?4.6mm,5?m)at a temperature of 25℃;the mobile phase was methyl-water-glacial acetic acid(40∶60∶0.4)at a flow velocity of 1.0mL?min-1,and the detection wavelength was set at 353nm.RESULTS:The linear concentration range of Esculetine was within 0.045～0.360?g(r=0.999 5).The average recovery rate was 97.72%(RSD=1.87%,n=5).CONCLUSION:This procedure is simple,accurate and reliable,and it can be used for the quality control of Dinglian tablets.