ABSTRACT
Nowadays, as the field of neural tissue engineering advances, the fabrication and application of combined structures open a new window of research for the regeneration of nervous system injuries. In this study, chitosan/poly [vinyl alcohol] -carbon nanotube nanocomposites has been exploited as scaffolds. Electrospinning was used to fabricate chitosan/poly [vinyl alcohol] -carbon nanotube scaffolds. Raman spectroscopy and scanning electron microscopy [SEM] was used to evaluate the chemical and physical structure of the electrospun scaffolds. Then, the biocompatibility of the scaffolds was evaluated using MTT assay and Neutral red assay. The results showed that the chitosan/poly [vinyl alcohol] -carbon nanotube nanocomposites have suitable structural and morphological aspects for human brain-derived cells growth and proliferation. Therefore, the cells could maintain their usual morphology while adhering to the surface of the nanocomposites due to an appropriate biocompatibility of the scaffolds. Chitosan/poly [vinyl alcohol] -carbon nanotube nanocomposites could enhance the proliferation of human brain-derived cells due to their proper structure and biocompatibility
ABSTRACT
Tissue engineering is an [interdisciplinary field that applies polymeric scaffolds to control tissue formation in three-dinemtion [3D]. The scaffold provides the microenvironment [synthetic temporary extracellular matrix] for regenerative cells, supporting cell attachment, proliferation, differentiation, and neo tissue genesis due to their suitable chemical, physical and biological structures. In this study, chitosan/poly [vinyl alcohol] [CS/PVA] was exploited as scaffold for nerve regeneration. Electrospinning was used to fabricate CS/PVA nanocomposites for U373 cells seeding and proliferation. Electrospinning is a versatile and simple method to fabricate non-woven thin layer fibers from polymeric solutions. Consequently, the biocompatibility of CS/PVA nanocomposite was evaluated using biological assays and cell attachment study. Results indicated that CS/PVA nanocomposites with 15/85 proportion shown an almost homogenous network of the electrospun fibers and confirmed that they can be knitted in meshes and improve U373 cells proliferation and cell attachment. The nano-sized CS/PVA scaffolds are nontoxic and biocompatible which can promote proliferation of U373 cells and their appropriate adhesion to nanocomposite for improved peripheral nerve regeneration